Changes in the Eye Microbiota Associated with Contact Lens Wearing
ABSTRACT Wearing contact lenses has been identified as a risk factor for the development of eye conditions such as giant papillary conjunctivitis and keratitis. We hypothesized that wearing contact lenses is associated with changes in the ocular microbiota. We compared the bacterial communities of t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2016-05-01
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| Series: | mBio |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.00198-16 |
| _version_ | 1818856350667505664 |
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| author | Hakdong Shin Kenneth Price Luong Albert Jack Dodick Lisa Park Maria Gloria Dominguez-Bello |
| author_facet | Hakdong Shin Kenneth Price Luong Albert Jack Dodick Lisa Park Maria Gloria Dominguez-Bello |
| author_sort | Hakdong Shin |
| collection | DOAJ |
| description | ABSTRACT Wearing contact lenses has been identified as a risk factor for the development of eye conditions such as giant papillary conjunctivitis and keratitis. We hypothesized that wearing contact lenses is associated with changes in the ocular microbiota. We compared the bacterial communities of the conjunctiva and skin under the eye from 58 subjects and analyzed samples from 20 subjects (9 lens wearers and 11 non-lens wearers) taken at 3 time points using a 16S rRNA gene-based sequencing technique (V4 region; Illumina MiSeq). We found that using anesthetic eye drops before sampling decreases the detected ocular microbiota diversity. Compared to those from non-lens wearers, dry conjunctival swabs from lens wearers had more variable and skin-like bacterial community structures (UniFrac; P value = <0.001), with higher abundances of Methylobacterium, Lactobacillus, Acinetobacter, and Pseudomonas and lower abundances of Haemophilus, Streptococcus, Staphylococcus, and Corynebacterium (linear discriminant analysis [LDA] score = >3.0). The results indicate that wearing contact lenses alters the microbial structure of the ocular conjunctiva, making it more similar to that of the skin microbiota. Further research is needed to determine whether the microbiome structure provides less protection from ocular infections. IMPORTANCE As in other body sites (i.e., the gut, skin, and mouth), the eye has a normal community of bacteria which are expected to confer resistance that provides protection from invaders. However, the eye microbiome has been largely neglected and is relevant to eye health and understanding eye diseases and to discovery of its functions. This report of a baseline study shows differences in the eye microbiome of contact lens wearers in relation to those of non-lens wearers and has the potential to help future studies explore novel insights into a possible role of the microbiome in the increased risk for eye infections in contact lens wearers. |
| first_indexed | 2024-12-19T08:23:06Z |
| format | Article |
| id | doaj.art-9cb94d59115c4b50a3fd75783ca5d879 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-19T08:23:06Z |
| publishDate | 2016-05-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-9cb94d59115c4b50a3fd75783ca5d8792022-12-21T20:29:22ZengAmerican Society for MicrobiologymBio2150-75112016-05-017210.1128/mBio.00198-16Changes in the Eye Microbiota Associated with Contact Lens WearingHakdong Shin0Kenneth Price1Luong Albert2Jack Dodick3Lisa Park4Maria Gloria Dominguez-Bello5Division of Translational Medicine, New York University School of Medicine, New York, New York, USADivision of Translational Medicine, New York University School of Medicine, New York, New York, USADivision of Translational Medicine, New York University School of Medicine, New York, New York, USADepartment of Ophthalmology, New York University School of Medicine, New York, New York, USADepartment of Ophthalmology, New York University School of Medicine, New York, New York, USADivision of Translational Medicine, New York University School of Medicine, New York, New York, USAABSTRACT Wearing contact lenses has been identified as a risk factor for the development of eye conditions such as giant papillary conjunctivitis and keratitis. We hypothesized that wearing contact lenses is associated with changes in the ocular microbiota. We compared the bacterial communities of the conjunctiva and skin under the eye from 58 subjects and analyzed samples from 20 subjects (9 lens wearers and 11 non-lens wearers) taken at 3 time points using a 16S rRNA gene-based sequencing technique (V4 region; Illumina MiSeq). We found that using anesthetic eye drops before sampling decreases the detected ocular microbiota diversity. Compared to those from non-lens wearers, dry conjunctival swabs from lens wearers had more variable and skin-like bacterial community structures (UniFrac; P value = <0.001), with higher abundances of Methylobacterium, Lactobacillus, Acinetobacter, and Pseudomonas and lower abundances of Haemophilus, Streptococcus, Staphylococcus, and Corynebacterium (linear discriminant analysis [LDA] score = >3.0). The results indicate that wearing contact lenses alters the microbial structure of the ocular conjunctiva, making it more similar to that of the skin microbiota. Further research is needed to determine whether the microbiome structure provides less protection from ocular infections. IMPORTANCE As in other body sites (i.e., the gut, skin, and mouth), the eye has a normal community of bacteria which are expected to confer resistance that provides protection from invaders. However, the eye microbiome has been largely neglected and is relevant to eye health and understanding eye diseases and to discovery of its functions. This report of a baseline study shows differences in the eye microbiome of contact lens wearers in relation to those of non-lens wearers and has the potential to help future studies explore novel insights into a possible role of the microbiome in the increased risk for eye infections in contact lens wearers.https://journals.asm.org/doi/10.1128/mBio.00198-16 |
| spellingShingle | Hakdong Shin Kenneth Price Luong Albert Jack Dodick Lisa Park Maria Gloria Dominguez-Bello Changes in the Eye Microbiota Associated with Contact Lens Wearing mBio |
| title | Changes in the Eye Microbiota Associated with Contact Lens Wearing |
| title_full | Changes in the Eye Microbiota Associated with Contact Lens Wearing |
| title_fullStr | Changes in the Eye Microbiota Associated with Contact Lens Wearing |
| title_full_unstemmed | Changes in the Eye Microbiota Associated with Contact Lens Wearing |
| title_short | Changes in the Eye Microbiota Associated with Contact Lens Wearing |
| title_sort | changes in the eye microbiota associated with contact lens wearing |
| url | https://journals.asm.org/doi/10.1128/mBio.00198-16 |
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