Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i>
<i>Variovorax</i> represents a widespread and ecologically significant genus of soil bacteria. Despite the ecological importance of these bacteria, our knowledge about the viruses infecting <i>Variovorax</i> spp. is quite poor. This study describes the isolation and character...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13539 |
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| author | Przemyslaw Decewicz Michal Kitowicz Monika Radlinska |
| author_facet | Przemyslaw Decewicz Michal Kitowicz Monika Radlinska |
| author_sort | Przemyslaw Decewicz |
| collection | DOAJ |
| description | <i>Variovorax</i> represents a widespread and ecologically significant genus of soil bacteria. Despite the ecological importance of these bacteria, our knowledge about the viruses infecting <i>Variovorax</i> spp. is quite poor. This study describes the isolation and characterization of the mitomycin-induced phage, named VarioGold. To the best of our knowledge, VarioGold represents the first characterized virus for this genus. Comparative genomic analyses suggested that VarioGold is distinct from currently known bacteriophages at both the nucleotide and protein levels; thus, it could be considered a new virus genus. In addition, another 37 prophages were distinguished in silico within the complete genomic sequences of <i>Variovorax</i> spp. that are available in public databases. The similarity networking analysis highlighted their general high diversity, which, despite clustering with previously described phages, shows their unique genetic load. Therefore, the novelty of <i>Variovorax</i> phages warrants the great enrichment of databases, which could, in turn, improve bioinformatic strategies for finding (pro)phages. |
| first_indexed | 2024-03-09T18:59:47Z |
| format | Article |
| id | doaj.art-9cb94f9144864ab1b1c1cfe96728d780 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:59:47Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9cb94f9144864ab1b1c1cfe96728d7802023-11-24T05:09:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211353910.3390/ijms232113539Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i>Przemyslaw Decewicz0Michal Kitowicz1Monika Radlinska2Department of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland<i>Variovorax</i> represents a widespread and ecologically significant genus of soil bacteria. Despite the ecological importance of these bacteria, our knowledge about the viruses infecting <i>Variovorax</i> spp. is quite poor. This study describes the isolation and characterization of the mitomycin-induced phage, named VarioGold. To the best of our knowledge, VarioGold represents the first characterized virus for this genus. Comparative genomic analyses suggested that VarioGold is distinct from currently known bacteriophages at both the nucleotide and protein levels; thus, it could be considered a new virus genus. In addition, another 37 prophages were distinguished in silico within the complete genomic sequences of <i>Variovorax</i> spp. that are available in public databases. The similarity networking analysis highlighted their general high diversity, which, despite clustering with previously described phages, shows their unique genetic load. Therefore, the novelty of <i>Variovorax</i> phages warrants the great enrichment of databases, which could, in turn, improve bioinformatic strategies for finding (pro)phages.https://www.mdpi.com/1422-0067/23/21/13539<i>Variovorax</i>bacteriophageprophagecomparative genomicZloty Stok |
| spellingShingle | Przemyslaw Decewicz Michal Kitowicz Monika Radlinska Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i> International Journal of Molecular Sciences <i>Variovorax</i> bacteriophage prophage comparative genomic Zloty Stok |
| title | Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i> |
| title_full | Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i> |
| title_fullStr | Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i> |
| title_full_unstemmed | Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i> |
| title_short | Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage of <i>Variovorax</i> |
| title_sort | characteristics and comparative genomic analysis of a novel virus variogold the first bacteriophage of i variovorax i |
| topic | <i>Variovorax</i> bacteriophage prophage comparative genomic Zloty Stok |
| url | https://www.mdpi.com/1422-0067/23/21/13539 |
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