Human Milk: Fast Determination of Docosahexaenoic Acid (DHA)
Human milk provides all the nutrients required by babies during the first six months of their life. Human milk lipids represent the main source of energy, contributing almost 50% of the total energy content. Additionally, fatty acids ensure the correct development of children in the prenatal, postna...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Analytica |
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| Online Access: | https://www.mdpi.com/2673-4532/4/1/6 |
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| author | Mahyara Markievicz Mancio Kus-Yamashita Cristiane Bonaldi Cano Vânia Claudia Barros Monteiro Regina Maria Catarino |
| author_facet | Mahyara Markievicz Mancio Kus-Yamashita Cristiane Bonaldi Cano Vânia Claudia Barros Monteiro Regina Maria Catarino |
| author_sort | Mahyara Markievicz Mancio Kus-Yamashita |
| collection | DOAJ |
| description | Human milk provides all the nutrients required by babies during the first six months of their life. Human milk lipids represent the main source of energy, contributing almost 50% of the total energy content. Additionally, fatty acids ensure the correct development of children in the prenatal, postnatal, and infant phases. Docosahexaenoic acid (DHA) is essential for visual and cognitive development, and its presence during childhood can affect long-term health. This study aimed to optimize and validate a methodology for the direct determination of DHA in human milk. We used 20 samples of human milk from lactating women living in the city of Itu, São Paulo, who attended Basic Health Units from September 2019 to September 2020, and a sample of certified reference material from the National Institute of Standards and Technology. The proposed methodology consists of a validated mixed transesterification process without prior lipid extraction, optimized by factorial design. This methodology can be successfully used in human milk samples as it is both precise and accurate. The values of DHA in the sampled milks were similar to those in European countries and lower than those in Asian countries due to diet. |
| first_indexed | 2024-03-11T07:02:45Z |
| format | Article |
| id | doaj.art-9cc3487bd3db499a89e568d1d9767e57 |
| institution | Directory Open Access Journal |
| issn | 2673-4532 |
| language | English |
| last_indexed | 2024-03-11T07:02:45Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Analytica |
| spelling | doaj.art-9cc3487bd3db499a89e568d1d9767e572023-11-17T09:09:51ZengMDPI AGAnalytica2673-45322023-03-0141546510.3390/analytica4010006Human Milk: Fast Determination of Docosahexaenoic Acid (DHA)Mahyara Markievicz Mancio Kus-Yamashita0Cristiane Bonaldi Cano1Vânia Claudia Barros Monteiro2Regina Maria Catarino3School of Pharmaceutical Science, University of São Paulo—USP, São Paulo 05508-000, BrazilChemistry, Physics and Sensory Core, Food Centre, Adolfo Lutz Institute, São Paulo 05508-000, BrazilMedical Assistance to the State Public Servant Institute (IAMSPE), São Paulo 05508-000, BrazilPathology Centre, Adolfo Lutz Institute, São Paulo 05508-000, BrazilHuman milk provides all the nutrients required by babies during the first six months of their life. Human milk lipids represent the main source of energy, contributing almost 50% of the total energy content. Additionally, fatty acids ensure the correct development of children in the prenatal, postnatal, and infant phases. Docosahexaenoic acid (DHA) is essential for visual and cognitive development, and its presence during childhood can affect long-term health. This study aimed to optimize and validate a methodology for the direct determination of DHA in human milk. We used 20 samples of human milk from lactating women living in the city of Itu, São Paulo, who attended Basic Health Units from September 2019 to September 2020, and a sample of certified reference material from the National Institute of Standards and Technology. The proposed methodology consists of a validated mixed transesterification process without prior lipid extraction, optimized by factorial design. This methodology can be successfully used in human milk samples as it is both precise and accurate. The values of DHA in the sampled milks were similar to those in European countries and lower than those in Asian countries due to diet.https://www.mdpi.com/2673-4532/4/1/6human milkpolyunsaturated acidbreastfeedinggas chromatographychemometric approach |
| spellingShingle | Mahyara Markievicz Mancio Kus-Yamashita Cristiane Bonaldi Cano Vânia Claudia Barros Monteiro Regina Maria Catarino Human Milk: Fast Determination of Docosahexaenoic Acid (DHA) Analytica human milk polyunsaturated acid breastfeeding gas chromatography chemometric approach |
| title | Human Milk: Fast Determination of Docosahexaenoic Acid (DHA) |
| title_full | Human Milk: Fast Determination of Docosahexaenoic Acid (DHA) |
| title_fullStr | Human Milk: Fast Determination of Docosahexaenoic Acid (DHA) |
| title_full_unstemmed | Human Milk: Fast Determination of Docosahexaenoic Acid (DHA) |
| title_short | Human Milk: Fast Determination of Docosahexaenoic Acid (DHA) |
| title_sort | human milk fast determination of docosahexaenoic acid dha |
| topic | human milk polyunsaturated acid breastfeeding gas chromatography chemometric approach |
| url | https://www.mdpi.com/2673-4532/4/1/6 |
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