The Application of an Omentum Graft or Flap in Spinal Cord Injury
Background: Spinal cord injury (SCI) causes a primary injury at the lesion site and triggers a secondary injury and prolonged inflammation. There has been no definitive treatment till now. Promoting angiogenesis is one of the most important strategies for functional recovery after SCI. The omentum,...
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/15/7930 |
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| author | Li-Yu Fay Yan-Ru Lin Dann-Ying Liou Chuan-Wen Chiu Mei-Yin Yeh Wen-Cheng Huang Jau-Ching Wu May-Jywan Tsai Henrich Cheng |
| author_facet | Li-Yu Fay Yan-Ru Lin Dann-Ying Liou Chuan-Wen Chiu Mei-Yin Yeh Wen-Cheng Huang Jau-Ching Wu May-Jywan Tsai Henrich Cheng |
| author_sort | Li-Yu Fay |
| collection | DOAJ |
| description | Background: Spinal cord injury (SCI) causes a primary injury at the lesion site and triggers a secondary injury and prolonged inflammation. There has been no definitive treatment till now. Promoting angiogenesis is one of the most important strategies for functional recovery after SCI. The omentum, abundant in blood and lymph vessels, possesses the potent ability of tissue regeneration. Methods: The present work examines the efficacy of autologous omentum, either as a flap (with vascular connection intact) or graft (severed vascular connection), on spinal nerve regeneration. After contusive SCI in rats, a thin sheath of omentum was grafted to the injured spinal cord. Results: Omental graft improved behavior scores significantly from the 3rd to 6th week after injury (6th week, 5.5 ± 0.5 vs. 8.6 ± 1.3, <i>p</i> < 0.05). Furthermore, the reduction in cavity and the preservation of class III β-tubulin-positive nerve fibers in the injury area was noted. Next, the free omental flap was transposed to a completely transected SCI in rats through a pre-implanted tunnel. The flap remained vascularized and survived well several weeks after the operation. At 16 weeks post-treatment, SCI rats with omentum flap treatment displayed the preservation of significantly more nerve fibers (<i>p</i> < 0.05) and a reduced injured cavity, though locomotor scores were similar. Conclusions: Taken together, the findings of this study indicate that treatment with an omental graft or transposition of an omental flap on an injured spinal cord has a positive effect on nerve protection and tissue preservation in SCI rats. The current data highlight the importance of omentum in clinical applications. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:15:09Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9cc37b309ed04b2d8e15aece567560082023-11-22T05:40:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012215793010.3390/ijms22157930The Application of an Omentum Graft or Flap in Spinal Cord InjuryLi-Yu Fay0Yan-Ru Lin1Dann-Ying Liou2Chuan-Wen Chiu3Mei-Yin Yeh4Wen-Cheng Huang5Jau-Ching Wu6May-Jywan Tsai7Henrich Cheng8Institute of Pharmacology, College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Taipei 11217, TaiwanDepartment of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Room 525, 17F, No. 201, Sec. 2, Shipai Road, Taipei 11217, TaiwanDepartment of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Room 525, 17F, No. 201, Sec. 2, Shipai Road, Taipei 11217, TaiwanDepartment of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Room 525, 17F, No. 201, Sec. 2, Shipai Road, Taipei 11217, TaiwanInstitute of Pharmacology, College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Taipei 11217, TaiwanInstitute of Pharmacology, College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Taipei 11217, TaiwanInstitute of Pharmacology, College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Taipei 11217, TaiwanDepartment of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Room 525, 17F, No. 201, Sec. 2, Shipai Road, Taipei 11217, TaiwanInstitute of Pharmacology, College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Taipei 11217, TaiwanBackground: Spinal cord injury (SCI) causes a primary injury at the lesion site and triggers a secondary injury and prolonged inflammation. There has been no definitive treatment till now. Promoting angiogenesis is one of the most important strategies for functional recovery after SCI. The omentum, abundant in blood and lymph vessels, possesses the potent ability of tissue regeneration. Methods: The present work examines the efficacy of autologous omentum, either as a flap (with vascular connection intact) or graft (severed vascular connection), on spinal nerve regeneration. After contusive SCI in rats, a thin sheath of omentum was grafted to the injured spinal cord. Results: Omental graft improved behavior scores significantly from the 3rd to 6th week after injury (6th week, 5.5 ± 0.5 vs. 8.6 ± 1.3, <i>p</i> < 0.05). Furthermore, the reduction in cavity and the preservation of class III β-tubulin-positive nerve fibers in the injury area was noted. Next, the free omental flap was transposed to a completely transected SCI in rats through a pre-implanted tunnel. The flap remained vascularized and survived well several weeks after the operation. At 16 weeks post-treatment, SCI rats with omentum flap treatment displayed the preservation of significantly more nerve fibers (<i>p</i> < 0.05) and a reduced injured cavity, though locomotor scores were similar. Conclusions: Taken together, the findings of this study indicate that treatment with an omental graft or transposition of an omental flap on an injured spinal cord has a positive effect on nerve protection and tissue preservation in SCI rats. The current data highlight the importance of omentum in clinical applications.https://www.mdpi.com/1422-0067/22/15/7930spinal cord injuryomentumlamininvascularizationtransplantation |
| spellingShingle | Li-Yu Fay Yan-Ru Lin Dann-Ying Liou Chuan-Wen Chiu Mei-Yin Yeh Wen-Cheng Huang Jau-Ching Wu May-Jywan Tsai Henrich Cheng The Application of an Omentum Graft or Flap in Spinal Cord Injury International Journal of Molecular Sciences spinal cord injury omentum laminin vascularization transplantation |
| title | The Application of an Omentum Graft or Flap in Spinal Cord Injury |
| title_full | The Application of an Omentum Graft or Flap in Spinal Cord Injury |
| title_fullStr | The Application of an Omentum Graft or Flap in Spinal Cord Injury |
| title_full_unstemmed | The Application of an Omentum Graft or Flap in Spinal Cord Injury |
| title_short | The Application of an Omentum Graft or Flap in Spinal Cord Injury |
| title_sort | application of an omentum graft or flap in spinal cord injury |
| topic | spinal cord injury omentum laminin vascularization transplantation |
| url | https://www.mdpi.com/1422-0067/22/15/7930 |
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