Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations
Background: Several compounds obtained from plants have potential insecticidal, growth deterrent or repellent characteristics. The control of hard ticks by non-chemical substances was targeted in this study. Methods: The effect of 36 materials on in-vitro ticks was studied, including 2 absolute cont...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-05-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/144 |
| _version_ | 1818141381768511488 |
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| author | Shawky Mohamed Aboelhadid Hesham A Mahran Hazem M El-Hariri Khalid Mohamed Shokier |
| author_facet | Shawky Mohamed Aboelhadid Hesham A Mahran Hazem M El-Hariri Khalid Mohamed Shokier |
| author_sort | Shawky Mohamed Aboelhadid |
| collection | DOAJ |
| description | Background: Several compounds obtained from plants have potential insecticidal, growth deterrent or repellent characteristics. The control of hard ticks by non-chemical substances was targeted in this study.
Methods: The effect of 36 materials on in-vitro ticks was studied, including 2 absolute controls (water only or absolute ethyl alcohol only), 6 conventionally used spinosad preparations (aqueous solutions), 12 Nigella sativa (N. sativa) preparations (aqueous and alcoholic solutions), and 12 Thyme preparations (aqueous and alcoholic solutions). The engorged ticks were tested in-vitro for mortality and oviposition ability using the studied materials.
Results: The final mortality after 48 hours of application in N. sativa aqueous preparations began from 10.0% concentration, 1.0% to 100% by concentration preparations ≥10%. In addition, N. sativa alcoholic preparations began from 50.0% concentration, 2 % to 100% by concentration ≥5%. Meanwhile, Thyme aqueous and alcoholic preparations began from 70.0% concentration, 5% to 90% by concentration 10–20%. Additionally, spinosad aqueous preparations and both of control preparations (Water and Alcohol) resulted in no mortality. All differences were statistically significant. The oviposition was stopped in N. sativa (aqueous ≥10% and alcoholic ≥5%) and in spinosad (aqueous≥25%). The aqoues dilution of the used matters killed B. annulatus larvae beginning from the concentration 5%.
Conclusion: Nigella sativa alcohol 20% was the best of studied preparations being the lowest concentration (20%) that could achieve the highest lethal (100%) effect in shortest time (12 hours). Moreover, Thyme oil and spinosad could not kill 100% of adult but did on larvae. |
| first_indexed | 2024-12-11T10:58:59Z |
| format | Article |
| id | doaj.art-9cc450ac128d43a5b26cf6b9e7c9ace8 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-11T10:58:59Z |
| publishDate | 2016-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-9cc450ac128d43a5b26cf6b9e7c9ace82022-12-22T01:09:57ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712016-05-01102131Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad PreparationsShawky Mohamed Aboelhadid0Hesham A Mahran1Hazem M El-Hariri2Khalid Mohamed Shokier3Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, EgyptDepartment of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, EgyptDepartment of Community Medicine Research, National Research Center, EgyptAnimal Health Research, Beni-Suef Branch, Beni-Suef, EgyptBackground: Several compounds obtained from plants have potential insecticidal, growth deterrent or repellent characteristics. The control of hard ticks by non-chemical substances was targeted in this study. Methods: The effect of 36 materials on in-vitro ticks was studied, including 2 absolute controls (water only or absolute ethyl alcohol only), 6 conventionally used spinosad preparations (aqueous solutions), 12 Nigella sativa (N. sativa) preparations (aqueous and alcoholic solutions), and 12 Thyme preparations (aqueous and alcoholic solutions). The engorged ticks were tested in-vitro for mortality and oviposition ability using the studied materials. Results: The final mortality after 48 hours of application in N. sativa aqueous preparations began from 10.0% concentration, 1.0% to 100% by concentration preparations ≥10%. In addition, N. sativa alcoholic preparations began from 50.0% concentration, 2 % to 100% by concentration ≥5%. Meanwhile, Thyme aqueous and alcoholic preparations began from 70.0% concentration, 5% to 90% by concentration 10–20%. Additionally, spinosad aqueous preparations and both of control preparations (Water and Alcohol) resulted in no mortality. All differences were statistically significant. The oviposition was stopped in N. sativa (aqueous ≥10% and alcoholic ≥5%) and in spinosad (aqueous≥25%). The aqoues dilution of the used matters killed B. annulatus larvae beginning from the concentration 5%. Conclusion: Nigella sativa alcohol 20% was the best of studied preparations being the lowest concentration (20%) that could achieve the highest lethal (100%) effect in shortest time (12 hours). Moreover, Thyme oil and spinosad could not kill 100% of adult but did on larvae.https://jad.tums.ac.ir/index.php/jad/article/view/144Rhipicephalus annulatusNigella sativaThyme oils |
| spellingShingle | Shawky Mohamed Aboelhadid Hesham A Mahran Hazem M El-Hariri Khalid Mohamed Shokier Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations Journal of Arthropod-Borne Diseases Rhipicephalus annulatus Nigella sativa Thyme oils |
| title | Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations |
| title_full | Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations |
| title_fullStr | Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations |
| title_full_unstemmed | Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations |
| title_short | Rhipicephalus annulatus (Acari: Ixodidae) Control by Nigella sativa, Thyme and Spinosad Preparations |
| title_sort | rhipicephalus annulatus acari ixodidae control by nigella sativa thyme and spinosad preparations |
| topic | Rhipicephalus annulatus Nigella sativa Thyme oils |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/144 |
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