Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK
Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) <i>E. coli</i> producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We...
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MDPI AG
2023-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/1/169 |
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| author | Delveen R. Ibrahim Christine E. R. Dodd Dov J. Stekel Remilekun T. Meshioye Mathew Diggle Michelle Lister Jon L. Hobman |
| author_facet | Delveen R. Ibrahim Christine E. R. Dodd Dov J. Stekel Remilekun T. Meshioye Mathew Diggle Michelle Lister Jon L. Hobman |
| author_sort | Delveen R. Ibrahim |
| collection | DOAJ |
| description | Globally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) <i>E. coli</i> producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We evaluated the occurrence of ESBL/pAmpC genetic determinants in phenotypically MDR <i>E. coli</i> isolated from clinical samples of blood, faeces, ear effusion, urine and sputum from a UK hospital. Phenotypic resistance profiling for 18 antibiotics (from seven classes) showed that 32/35 isolates were MDR, with resistance to 4–16 of the tested antibiotics. Of the isolates, 97.1% showed resistance to ampicillin, 71.4% showed resistance to co-amoxiclav, cefotaxime, ceftazidime and ceftiofur, and 68.5% showed resistance to cefquinome. <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>OXA-1</sub> genes were detected in 23, 13 and 12 strains, respectively, and <i>Intl1</i> was detected in 17 isolates. The most common subtypes among the definite sequence types were CTX-M-15 (40%) and TEM-1 (75%). No <i>E. coli</i> isolates carried pAmpC genes. Significant correlations were seen between CTX-M carriage and cefotaxime, ceftiofur, aztreonam, ceftazidime and cefquinome resistance; between <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>OXA-1</sub> carriage and ciprofloxacin resistance; and between <i>Intl1</i> carriage and trimethoprim/sulfamethoxazole resistance. Thus, MDR phenotypes may be conferred by a relatively small number of genes. The level and pattern of antibiotic resistance highlight the need for better antibiotic therapy guidelines, including reduced use and improved surveillance. |
| first_indexed | 2024-03-09T13:47:23Z |
| format | Article |
| id | doaj.art-9cc51c723dcf456486d0cd90617a2ee8 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T13:47:23Z |
| publishDate | 2023-01-01 |
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| series | Antibiotics |
| spelling | doaj.art-9cc51c723dcf456486d0cd90617a2ee82023-11-30T20:56:43ZengMDPI AGAntibiotics2079-63822023-01-0112116910.3390/antibiotics12010169Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UKDelveen R. Ibrahim0Christine E. R. Dodd1Dov J. Stekel2Remilekun T. Meshioye3Mathew Diggle4Michelle Lister5Jon L. Hobman6Department of Biology, School of Science, The University of Duhok, Duhok 42001, IraqSchool of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, UKSchool of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, UKSchool of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, UKAlberta Health Services, Edmonton, AB T6G 2J2, CanadaDepartment of Microbiology, Nottingham University Hospitals NHS Trust, Derby Road, Nottingham NG7 2UH, UKSchool of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, UKGlobally, cephalosporin therapy failure is a serious problem for infection control. One causative agent of cephalosporin-resistant infections is multidrug-resistant (MDR) <i>E. coli</i> producing extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases. We evaluated the occurrence of ESBL/pAmpC genetic determinants in phenotypically MDR <i>E. coli</i> isolated from clinical samples of blood, faeces, ear effusion, urine and sputum from a UK hospital. Phenotypic resistance profiling for 18 antibiotics (from seven classes) showed that 32/35 isolates were MDR, with resistance to 4–16 of the tested antibiotics. Of the isolates, 97.1% showed resistance to ampicillin, 71.4% showed resistance to co-amoxiclav, cefotaxime, ceftazidime and ceftiofur, and 68.5% showed resistance to cefquinome. <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>OXA-1</sub> genes were detected in 23, 13 and 12 strains, respectively, and <i>Intl1</i> was detected in 17 isolates. The most common subtypes among the definite sequence types were CTX-M-15 (40%) and TEM-1 (75%). No <i>E. coli</i> isolates carried pAmpC genes. Significant correlations were seen between CTX-M carriage and cefotaxime, ceftiofur, aztreonam, ceftazidime and cefquinome resistance; between <i>bla</i><sub>CTX-M</sub>, <i>bla</i><sub>TEM</sub> and <i>bla</i><sub>OXA-1</sub> carriage and ciprofloxacin resistance; and between <i>Intl1</i> carriage and trimethoprim/sulfamethoxazole resistance. Thus, MDR phenotypes may be conferred by a relatively small number of genes. The level and pattern of antibiotic resistance highlight the need for better antibiotic therapy guidelines, including reduced use and improved surveillance.https://www.mdpi.com/2079-6382/12/1/169<i>Escherichia coli</i>multidrug-resistantcephalosporin-resistantESBL |
| spellingShingle | Delveen R. Ibrahim Christine E. R. Dodd Dov J. Stekel Remilekun T. Meshioye Mathew Diggle Michelle Lister Jon L. Hobman Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK Antibiotics <i>Escherichia coli</i> multidrug-resistant cephalosporin-resistant ESBL |
| title | Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK |
| title_full | Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK |
| title_fullStr | Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK |
| title_full_unstemmed | Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK |
| title_short | Multidrug-Resistant ESBL-Producing <i>E. coli</i> in Clinical Samples from the UK |
| title_sort | multidrug resistant esbl producing i e coli i in clinical samples from the uk |
| topic | <i>Escherichia coli</i> multidrug-resistant cephalosporin-resistant ESBL |
| url | https://www.mdpi.com/2079-6382/12/1/169 |
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