Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells
Summary: Epithelial-mesenchymal transition (EMT) changes cell phenotype by affecting immune properties of amniotic epithelial cells (AECs). The present study shows how the response to lipopolysaccharide of cells collected pre- (eAECs) and post-EMT (mAECs) induces changes in their transcriptomics pro...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223016590 |
| _version_ | 1797732874943725568 |
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| author | Valeria Di Lollo Angelo Canciello Alessia Peserico Massimiliano Orsini Valentina Russo Adrián Cerveró-Varona Beatrice Dufrusine Mohammad El Khatib Valentina Curini Annunziata Mauro Paolo Berardinelli Cathy Tournier Massimo Ancora Cesare Cammà Enrico Dainese Luana Fiorella Mincarelli Barbara Barboni |
| author_facet | Valeria Di Lollo Angelo Canciello Alessia Peserico Massimiliano Orsini Valentina Russo Adrián Cerveró-Varona Beatrice Dufrusine Mohammad El Khatib Valentina Curini Annunziata Mauro Paolo Berardinelli Cathy Tournier Massimo Ancora Cesare Cammà Enrico Dainese Luana Fiorella Mincarelli Barbara Barboni |
| author_sort | Valeria Di Lollo |
| collection | DOAJ |
| description | Summary: Epithelial-mesenchymal transition (EMT) changes cell phenotype by affecting immune properties of amniotic epithelial cells (AECs). The present study shows how the response to lipopolysaccharide of cells collected pre- (eAECs) and post-EMT (mAECs) induces changes in their transcriptomics profile. In fact, eAECs mainly upregulate genes involved in antigen-presenting response, whereas mAECs over-express soluble inflammatory mediator transcripts. Consistently, network analysis identifies CIITA and Nrf2 as main drivers of eAECs and mAECs immune response, respectively. As a consequence, the depletion of CIITA and Nrf2 impairs the ability of eAECs and mAECs to inhibit lymphocyte proliferation or macrophage-dependent IL-6 release, thus confirming their involvement in regulating immune response. Deciphering the mechanisms controlling the immune function of AECs pre- and post-EMT represents a step forward in understanding key physiological events wherein these cells are involved (pregnancy and labor). Moreover, controlling the immunomodulatory properties of eAECs and mAECs may be essential in developing potential strategies for regenerative medicine applications. |
| first_indexed | 2024-03-12T12:19:50Z |
| format | Article |
| id | doaj.art-9cc52941374346d09d0194e47c25d72a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T12:19:50Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9cc52941374346d09d0194e47c25d72a2023-08-30T05:54:35ZengElsevieriScience2589-00422023-09-01269107582Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cellsValeria Di Lollo0Angelo Canciello1Alessia Peserico2Massimiliano Orsini3Valentina Russo4Adrián Cerveró-Varona5Beatrice Dufrusine6Mohammad El Khatib7Valentina Curini8Annunziata Mauro9Paolo Berardinelli10Cathy Tournier11Massimo Ancora12Cesare Cammà13Enrico Dainese14Luana Fiorella Mincarelli15Barbara Barboni16National Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, Italy; Corresponding authorNational Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy; Istituto Zooprofilattico Sperimentale delle Venezie, Department of Microbiology, Viale dell’Università 10, 35020 Legnaro (PD), ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyNational Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyDivision of Cancer Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PL, UKNational Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyNational Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalyNational Reference Center for Whole Genome Sequencing of Microbial Pathogens: Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Campo Boario, 64100 Teramo, ItalyDepartment of Biosciences and Technology for Food, Agriculture and Environment, University of Teramo, Via Balzarini 1, 64100 Teramo, ItalySummary: Epithelial-mesenchymal transition (EMT) changes cell phenotype by affecting immune properties of amniotic epithelial cells (AECs). The present study shows how the response to lipopolysaccharide of cells collected pre- (eAECs) and post-EMT (mAECs) induces changes in their transcriptomics profile. In fact, eAECs mainly upregulate genes involved in antigen-presenting response, whereas mAECs over-express soluble inflammatory mediator transcripts. Consistently, network analysis identifies CIITA and Nrf2 as main drivers of eAECs and mAECs immune response, respectively. As a consequence, the depletion of CIITA and Nrf2 impairs the ability of eAECs and mAECs to inhibit lymphocyte proliferation or macrophage-dependent IL-6 release, thus confirming their involvement in regulating immune response. Deciphering the mechanisms controlling the immune function of AECs pre- and post-EMT represents a step forward in understanding key physiological events wherein these cells are involved (pregnancy and labor). Moreover, controlling the immunomodulatory properties of eAECs and mAECs may be essential in developing potential strategies for regenerative medicine applications.http://www.sciencedirect.com/science/article/pii/S2589004223016590ImmunologyCell biologyStem cells researchTranscriptomics |
| spellingShingle | Valeria Di Lollo Angelo Canciello Alessia Peserico Massimiliano Orsini Valentina Russo Adrián Cerveró-Varona Beatrice Dufrusine Mohammad El Khatib Valentina Curini Annunziata Mauro Paolo Berardinelli Cathy Tournier Massimo Ancora Cesare Cammà Enrico Dainese Luana Fiorella Mincarelli Barbara Barboni Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells iScience Immunology Cell biology Stem cells research Transcriptomics |
| title | Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells |
| title_full | Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells |
| title_fullStr | Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells |
| title_full_unstemmed | Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells |
| title_short | Unveiling the immunomodulatory shift: Epithelial-mesenchymal transition Alters immune mechanisms of amniotic epithelial cells |
| title_sort | unveiling the immunomodulatory shift epithelial mesenchymal transition alters immune mechanisms of amniotic epithelial cells |
| topic | Immunology Cell biology Stem cells research Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223016590 |
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