Tectomer-Mediated Optical Nanosensors for Tyramine Determination
The development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is c...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/5/2524 |
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| author | Mario Domínguez Sofía Oliver Rosa Garriga Edgar Muñoz Vicente L. Cebolla Susana de Marcos Javier Galbán |
| author_facet | Mario Domínguez Sofía Oliver Rosa Garriga Edgar Muñoz Vicente L. Cebolla Susana de Marcos Javier Galbán |
| author_sort | Mario Domínguez |
| collection | DOAJ |
| description | The development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is commonly associated with food spoilage) when coupled to Au(III)/tectomer films deposited on polylactic acid (PLA) supports. Tectomers are two-dimensional oligoglycine self-assemblies, whose terminal amino groups enable both the immobilization of Au(III) and its adhesion to PLA. Upon exposure to tyramine, a non-enzymatic redox reaction takes place in which Au(III) in the tectomer matrix is reduced by tyramine to gold nanoparticles, whose reddish-purple color depends on the tyramine concentration and can be identified by measuring the RGB coordinates (Red–Green–Blue coordinates) using a smartphone color recognition app. Moreover, a more accurate quantification of tyramine in the range from 0.048 to 10 μM could be performed by measuring the reflectance of the sensing layers and the absorbance of the characteristic 550 nm plasmon band of the gold nanoparticles. The relative standard deviation (RSD) of the method was 4.2% (<i>n</i> = 5) with a limit of detection (LOD) of 0.014 μM. A remarkable selectivity was achieved for tyramine detection in the presence of other biogenic amines, especially histamine. This methodology, based on the optical properties of Au(III)/tectomer hybrid coatings, is promising for its application in food quality control and smart food packaging. |
| first_indexed | 2024-03-11T07:10:39Z |
| format | Article |
| id | doaj.art-9cc586ee356841119c107b4de72cfa93 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T07:10:39Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9cc586ee356841119c107b4de72cfa932023-11-17T08:35:59ZengMDPI AGSensors1424-82202023-02-01235252410.3390/s23052524Tectomer-Mediated Optical Nanosensors for Tyramine DeterminationMario Domínguez0Sofía Oliver1Rosa Garriga2Edgar Muñoz3Vicente L. Cebolla4Susana de Marcos5Javier Galbán6Nanosensors and Bioanalytical Systems (N&SB), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA University of Zaragoza-CSIC), 50009 Zaragoza, SpainNanosensors and Bioanalytical Systems (N&SB), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA University of Zaragoza-CSIC), 50009 Zaragoza, SpainDepartamento de Química-Física, University of Zaragoza, 50009 Zaragoza, SpainInstituto de Carboquímica ICB-CSIC, 50018 Zaragoza, SpainInstituto de Carboquímica ICB-CSIC, 50018 Zaragoza, SpainNanosensors and Bioanalytical Systems (N&SB), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA University of Zaragoza-CSIC), 50009 Zaragoza, SpainNanosensors and Bioanalytical Systems (N&SB), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA University of Zaragoza-CSIC), 50009 Zaragoza, SpainThe development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is commonly associated with food spoilage) when coupled to Au(III)/tectomer films deposited on polylactic acid (PLA) supports. Tectomers are two-dimensional oligoglycine self-assemblies, whose terminal amino groups enable both the immobilization of Au(III) and its adhesion to PLA. Upon exposure to tyramine, a non-enzymatic redox reaction takes place in which Au(III) in the tectomer matrix is reduced by tyramine to gold nanoparticles, whose reddish-purple color depends on the tyramine concentration and can be identified by measuring the RGB coordinates (Red–Green–Blue coordinates) using a smartphone color recognition app. Moreover, a more accurate quantification of tyramine in the range from 0.048 to 10 μM could be performed by measuring the reflectance of the sensing layers and the absorbance of the characteristic 550 nm plasmon band of the gold nanoparticles. The relative standard deviation (RSD) of the method was 4.2% (<i>n</i> = 5) with a limit of detection (LOD) of 0.014 μM. A remarkable selectivity was achieved for tyramine detection in the presence of other biogenic amines, especially histamine. This methodology, based on the optical properties of Au(III)/tectomer hybrid coatings, is promising for its application in food quality control and smart food packaging.https://www.mdpi.com/1424-8220/23/5/2524tyraminebiogenic aminesgold nanoparticlesRGB coordinatesoptical sensorsfood quality control |
| spellingShingle | Mario Domínguez Sofía Oliver Rosa Garriga Edgar Muñoz Vicente L. Cebolla Susana de Marcos Javier Galbán Tectomer-Mediated Optical Nanosensors for Tyramine Determination Sensors tyramine biogenic amines gold nanoparticles RGB coordinates optical sensors food quality control |
| title | Tectomer-Mediated Optical Nanosensors for Tyramine Determination |
| title_full | Tectomer-Mediated Optical Nanosensors for Tyramine Determination |
| title_fullStr | Tectomer-Mediated Optical Nanosensors for Tyramine Determination |
| title_full_unstemmed | Tectomer-Mediated Optical Nanosensors for Tyramine Determination |
| title_short | Tectomer-Mediated Optical Nanosensors for Tyramine Determination |
| title_sort | tectomer mediated optical nanosensors for tyramine determination |
| topic | tyramine biogenic amines gold nanoparticles RGB coordinates optical sensors food quality control |
| url | https://www.mdpi.com/1424-8220/23/5/2524 |
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