Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis
IntroductionMycoplasma anserisalpingitidis is one of the most important waterfowl-pathogenic mycoplasmas. Due to inadequate antibiotic treatment, many strains with high minimal inhibitory concentration (MIC) values for multiple drugs have been isolated lately. Decreased antibiotic susceptibility in...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1216893/full |
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| author | Eszter Zsófia Nagy Eszter Zsófia Nagy Áron Botond Kovács Áron Botond Kovács Enikő Wehmann Enikő Wehmann Katinka Bekő Dorottya Földi Dorottya Földi Krisztián Bányai Krisztián Bányai Krisztián Bányai Zsuzsa Kreizinger Zsuzsa Kreizinger Miklós Gyuranecz Miklós Gyuranecz Miklós Gyuranecz Miklós Gyuranecz |
| author_facet | Eszter Zsófia Nagy Eszter Zsófia Nagy Áron Botond Kovács Áron Botond Kovács Enikő Wehmann Enikő Wehmann Katinka Bekő Dorottya Földi Dorottya Földi Krisztián Bányai Krisztián Bányai Krisztián Bányai Zsuzsa Kreizinger Zsuzsa Kreizinger Miklós Gyuranecz Miklós Gyuranecz Miklós Gyuranecz Miklós Gyuranecz |
| author_sort | Eszter Zsófia Nagy |
| collection | DOAJ |
| description | IntroductionMycoplasma anserisalpingitidis is one of the most important waterfowl-pathogenic mycoplasmas. Due to inadequate antibiotic treatment, many strains with high minimal inhibitory concentration (MIC) values for multiple drugs have been isolated lately. Decreased antibiotic susceptibility in several Mycoplasma species are known to be associated with mutations in topoisomerase and ribosomal genes, but other strategies such as active efflux pump mechanisms were also described. The scope of this study was the phenotypic and genetic characterization of the active efflux mechanism in M. anserisalpingitidisMethodsWe measured the MIC values in the presence and absence of different efflux pump inhibitors (EPIs), such as carbonyl cyanide m-chlorophenylhydrazine (CCCP), orthovanadate (OV), and reserpine (RSP). Moreover, bioinformatic tools were utilized to detect putative regulatory sequences of membrane transport proteins coding genes, while comparative genome analysis was performed to reveal potential markers of antibiotic resistance.ResultsOut of the three examined EPIs, CCCP decreased the MICs at least two-fold below the original MICs (in 23 cases out of 36 strains). In the presence of OV or RSP, MIC value differences could be seen only if modified dilution series (10% decrease steps were used instead of two-fold dilutions) were applied (in 24/36 cases with OV and 9/36 with RSP). During comparative genome analysis, non-synonymous single nucleotide polymorphisms (nsSNPs) were identified in genes encoding ABC membrane transport proteins, which were displayed in higher percentages in M. anserisalpingitidis strains with increased MICs. In terms of other genes, a nsSNP was identified in DNA gyrase subunit A (gyrA) gene which can be related to decreased susceptibility to enrofloxacin. The present study is the first to highlight the importance of efflux pump mechanisms in M. anserisalpingitidis.DiscussionConsidering the observed effects of the EPI CCCP against this bacterium, it can be assumed, that the use of EPIs would increase the efficiency of targeted antibiotic therapy in the future control of this pathogen. However, further research is required to obtain a more comprehensive understanding of efflux pump mechanism in this bacterium. |
| first_indexed | 2024-03-13T00:05:59Z |
| format | Article |
| id | doaj.art-9cc7662de27543fe9eeca94ad649b1da |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T00:05:59Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9cc7662de27543fe9eeca94ad649b1da2023-07-13T01:13:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.12168931216893Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidisEszter Zsófia Nagy0Eszter Zsófia Nagy1Áron Botond Kovács2Áron Botond Kovács3Enikő Wehmann4Enikő Wehmann5Katinka Bekő6Dorottya Földi7Dorottya Földi8Krisztián Bányai9Krisztián Bányai10Krisztián Bányai11Zsuzsa Kreizinger12Zsuzsa Kreizinger13Miklós Gyuranecz14Miklós Gyuranecz15Miklós Gyuranecz16Miklós Gyuranecz17Veterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, HungaryDepartment of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, HungaryVeterinary Medical Research Institute, Budapest, HungaryMolliScience Kft., Biatorbágy, HungaryVeterinary Medical Research Institute, Budapest, HungaryNational Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, HungaryMolliScience Kft., Biatorbágy, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, HungaryIntroductionMycoplasma anserisalpingitidis is one of the most important waterfowl-pathogenic mycoplasmas. Due to inadequate antibiotic treatment, many strains with high minimal inhibitory concentration (MIC) values for multiple drugs have been isolated lately. Decreased antibiotic susceptibility in several Mycoplasma species are known to be associated with mutations in topoisomerase and ribosomal genes, but other strategies such as active efflux pump mechanisms were also described. The scope of this study was the phenotypic and genetic characterization of the active efflux mechanism in M. anserisalpingitidisMethodsWe measured the MIC values in the presence and absence of different efflux pump inhibitors (EPIs), such as carbonyl cyanide m-chlorophenylhydrazine (CCCP), orthovanadate (OV), and reserpine (RSP). Moreover, bioinformatic tools were utilized to detect putative regulatory sequences of membrane transport proteins coding genes, while comparative genome analysis was performed to reveal potential markers of antibiotic resistance.ResultsOut of the three examined EPIs, CCCP decreased the MICs at least two-fold below the original MICs (in 23 cases out of 36 strains). In the presence of OV or RSP, MIC value differences could be seen only if modified dilution series (10% decrease steps were used instead of two-fold dilutions) were applied (in 24/36 cases with OV and 9/36 with RSP). During comparative genome analysis, non-synonymous single nucleotide polymorphisms (nsSNPs) were identified in genes encoding ABC membrane transport proteins, which were displayed in higher percentages in M. anserisalpingitidis strains with increased MICs. In terms of other genes, a nsSNP was identified in DNA gyrase subunit A (gyrA) gene which can be related to decreased susceptibility to enrofloxacin. The present study is the first to highlight the importance of efflux pump mechanisms in M. anserisalpingitidis.DiscussionConsidering the observed effects of the EPI CCCP against this bacterium, it can be assumed, that the use of EPIs would increase the efficiency of targeted antibiotic therapy in the future control of this pathogen. However, further research is required to obtain a more comprehensive understanding of efflux pump mechanism in this bacterium.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1216893/fullMycoplasma sp. 1220efflux pump inhibitorsantimicrobial resistanceABC efflux pumpminimal inhibitory concentration |
| spellingShingle | Eszter Zsófia Nagy Eszter Zsófia Nagy Áron Botond Kovács Áron Botond Kovács Enikő Wehmann Enikő Wehmann Katinka Bekő Dorottya Földi Dorottya Földi Krisztián Bányai Krisztián Bányai Krisztián Bányai Zsuzsa Kreizinger Zsuzsa Kreizinger Miklós Gyuranecz Miklós Gyuranecz Miklós Gyuranecz Miklós Gyuranecz Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis Frontiers in Microbiology Mycoplasma sp. 1220 efflux pump inhibitors antimicrobial resistance ABC efflux pump minimal inhibitory concentration |
| title | Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis |
| title_full | Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis |
| title_fullStr | Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis |
| title_full_unstemmed | Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis |
| title_short | Phenotypic and genetic insights into efflux pump mechanism in Mycoplasma anserisalpingitidis |
| title_sort | phenotypic and genetic insights into efflux pump mechanism in mycoplasma anserisalpingitidis |
| topic | Mycoplasma sp. 1220 efflux pump inhibitors antimicrobial resistance ABC efflux pump minimal inhibitory concentration |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1216893/full |
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