Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement
The present study focuses on the stress response of a filamentous, AT-rich, heterocystous cyanobacterium Mastigocladus laminosus UU774, isolated from a hot spring, Taptapani, located in the eastern part of India. The genome of UU774 contains an indispensable fragment, scaffold_38, of unknown origin...
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| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.909289/full |
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| author | Mayuri Mukherjee Mayuri Mukherjee Aribam Geeta Aribam Geeta Samrat Ghosh Samrat Ghosh Asharani Prusty Asharani Prusty Subhajeet Dutta Subhajeet Dutta Aditya Narayan Sarangi Smrutisanjita Behera Siba Prasad Adhikary Sucheta Tripathy Sucheta Tripathy |
| author_facet | Mayuri Mukherjee Mayuri Mukherjee Aribam Geeta Aribam Geeta Samrat Ghosh Samrat Ghosh Asharani Prusty Asharani Prusty Subhajeet Dutta Subhajeet Dutta Aditya Narayan Sarangi Smrutisanjita Behera Siba Prasad Adhikary Sucheta Tripathy Sucheta Tripathy |
| author_sort | Mayuri Mukherjee |
| collection | DOAJ |
| description | The present study focuses on the stress response of a filamentous, AT-rich, heterocystous cyanobacterium Mastigocladus laminosus UU774, isolated from a hot spring, Taptapani, located in the eastern part of India. The genome of UU774 contains an indispensable fragment, scaffold_38, of unknown origin that is implicated during severe nitrogen and nutrition stress. Prolonged exposure to nitrogen compounds during starvation has profound adverse effects on UU774, leading to loss of mobility, loss of ability to fight pathogens, reduced cell division, decreased nitrogen-fixing ability, reduced ability to form biofilms, reduced photosynthetic and light-sensing ability, and reduced production of secreted effectors and chromosomal toxin genes, among others. Among genes showing extreme downregulation when grown in a medium supplemented with nitrogen with the fold change > 5 are transcriptional regulator gene WalR, carbonic anhydrases, RNA Polymerase Sigma F factor, fimbrial protein, and twitching mobility protein. The reduced expression of key enzymes involved in the uptake of phosphate and enzymes protecting oxygen-sensitive nitrogenases is significant during the presence of nitrogen. UU774 is presumed to withstand heat by overexpressing peptidases that may be degrading abnormally folded proteins produced during heat. The absence of a key gene responsible for heterocyst pattern formation, patS, and an aberrant hetN without a functional motif probably lead to the formation of a chaotic heterocyst pattern in UU774. We suggest that UU774 has diverged from Fischerella sp. PCC 9339, another hot spring species isolated in the United States. |
| first_indexed | 2024-12-12T06:30:06Z |
| format | Article |
| id | doaj.art-9cc90bc0a99c4bd39b0849cf346b524c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T06:30:06Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9cc90bc0a99c4bd39b0849cf346b524c2022-12-22T00:34:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-07-011310.3389/fmicb.2022.909289909289Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen SupplementMayuri Mukherjee0Mayuri Mukherjee1Aribam Geeta2Aribam Geeta3Samrat Ghosh4Samrat Ghosh5Asharani Prusty6Asharani Prusty7Subhajeet Dutta8Subhajeet Dutta9Aditya Narayan Sarangi10Smrutisanjita Behera11Siba Prasad Adhikary12Sucheta Tripathy13Sucheta Tripathy14Computational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaDepartment of Biotechnology, Siksha Bhavana, Visva-Bharati, Santiniketan, IndiaComputational Genomics Lab, Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaThe present study focuses on the stress response of a filamentous, AT-rich, heterocystous cyanobacterium Mastigocladus laminosus UU774, isolated from a hot spring, Taptapani, located in the eastern part of India. The genome of UU774 contains an indispensable fragment, scaffold_38, of unknown origin that is implicated during severe nitrogen and nutrition stress. Prolonged exposure to nitrogen compounds during starvation has profound adverse effects on UU774, leading to loss of mobility, loss of ability to fight pathogens, reduced cell division, decreased nitrogen-fixing ability, reduced ability to form biofilms, reduced photosynthetic and light-sensing ability, and reduced production of secreted effectors and chromosomal toxin genes, among others. Among genes showing extreme downregulation when grown in a medium supplemented with nitrogen with the fold change > 5 are transcriptional regulator gene WalR, carbonic anhydrases, RNA Polymerase Sigma F factor, fimbrial protein, and twitching mobility protein. The reduced expression of key enzymes involved in the uptake of phosphate and enzymes protecting oxygen-sensitive nitrogenases is significant during the presence of nitrogen. UU774 is presumed to withstand heat by overexpressing peptidases that may be degrading abnormally folded proteins produced during heat. The absence of a key gene responsible for heterocyst pattern formation, patS, and an aberrant hetN without a functional motif probably lead to the formation of a chaotic heterocyst pattern in UU774. We suggest that UU774 has diverged from Fischerella sp. PCC 9339, another hot spring species isolated in the United States.https://www.frontiersin.org/articles/10.3389/fmicb.2022.909289/fullhot spring cyanobacterianutrient and nitrogen stressheat shocknitrogen fixationHapalosiphonaceae |
| spellingShingle | Mayuri Mukherjee Mayuri Mukherjee Aribam Geeta Aribam Geeta Samrat Ghosh Samrat Ghosh Asharani Prusty Asharani Prusty Subhajeet Dutta Subhajeet Dutta Aditya Narayan Sarangi Smrutisanjita Behera Siba Prasad Adhikary Sucheta Tripathy Sucheta Tripathy Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement Frontiers in Microbiology hot spring cyanobacteria nutrient and nitrogen stress heat shock nitrogen fixation Hapalosiphonaceae |
| title | Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement |
| title_full | Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement |
| title_fullStr | Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement |
| title_full_unstemmed | Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement |
| title_short | Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring Cyanobacterium Mastigocladus laminosus UU774 Under Exogenous Nitrogen Supplement |
| title_sort | genome analysis coupled with transcriptomics reveals the reduced fitness of a hot spring cyanobacterium mastigocladus laminosus uu774 under exogenous nitrogen supplement |
| topic | hot spring cyanobacteria nutrient and nitrogen stress heat shock nitrogen fixation Hapalosiphonaceae |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.909289/full |
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