In vitro Effect of Silver Nanoparticles on Blastocystis hominis
Mohamed Saad Younis,1 Eman Abd el rahman Abououf,1 Ali El saeed Ali,1 Sara Mohamed Abd elhady,2 Rita Maher Wassef2 1Medical Parasitology Department, Faculty of Medicine, Benha University, Benha, Egypt; 2Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, EgyptCorresponden...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-10-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/in-vitro-effect-of-silver-nanoparticles-on-blastocystis-hominis-peer-reviewed-article-IJN |
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| author | Younis SA Abououf EA Ali AE Abd elhady SM Wassef RM |
| author_facet | Younis SA Abououf EA Ali AE Abd elhady SM Wassef RM |
| author_sort | Younis SA |
| collection | DOAJ |
| description | Mohamed Saad Younis,1 Eman Abd el rahman Abououf,1 Ali El saeed Ali,1 Sara Mohamed Abd elhady,2 Rita Maher Wassef2 1Medical Parasitology Department, Faculty of Medicine, Benha University, Benha, Egypt; 2Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, EgyptCorrespondence: Rita Maher WassefMedical Parasitology Department, Faculty of Medicine, Helwan University, 30 Street 276 New Maadi, Cairo, EgyptTel +201005782994Fax +20225193381Email rita.wassef@med.helwan.edu.egIntroduction: This study aims to assess the efficacy of silver nanoparticles (Ag Nps) alone and combined with metronidazole (Ag Nps + MTZ) as potential alternative therapeutic agents for Blastocystis hominis.Methods: The parasites were challenged with Ag Nps, Ag Nps + MTZ and MTZ. To assess the efficacy of drugs, counting of viable parasites was done after 1, 2, and 3 hours of adding the drugs.Results: Blastocystis hominis count was reduced by 20.72%, 28.23%, and 18.92% after one hour of adding Ag Nps, Ag Nps + MTZ, and MTZ, respectively. Cysts count was further reduced by 51.49%, 61.61%, and 40.78% after 2 hours and by 71.69%, 79.67%, and 62.65% after 3 hours of adding the drugs in the same order, respectively.Conclusion: There was a statistically significant difference (P< 0.05) in the in vitro growth inhibition of the parasite over the different time intervals when using the tested drugs against the control drug.Keywords: Blastocystis hominis, eosin brilliant cresyl blue stain, metronidazole, silver nanoparticles |
| first_indexed | 2024-12-19T08:48:01Z |
| format | Article |
| id | doaj.art-9cca3760c07340acab33ce74410a4918 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T08:48:01Z |
| publishDate | 2020-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-9cca3760c07340acab33ce74410a49182022-12-21T20:28:46ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-10-01Volume 158167817358392In vitro Effect of Silver Nanoparticles on Blastocystis hominisYounis SAAbououf EAAli AEAbd elhady SMWassef RMMohamed Saad Younis,1 Eman Abd el rahman Abououf,1 Ali El saeed Ali,1 Sara Mohamed Abd elhady,2 Rita Maher Wassef2 1Medical Parasitology Department, Faculty of Medicine, Benha University, Benha, Egypt; 2Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, EgyptCorrespondence: Rita Maher WassefMedical Parasitology Department, Faculty of Medicine, Helwan University, 30 Street 276 New Maadi, Cairo, EgyptTel +201005782994Fax +20225193381Email rita.wassef@med.helwan.edu.egIntroduction: This study aims to assess the efficacy of silver nanoparticles (Ag Nps) alone and combined with metronidazole (Ag Nps + MTZ) as potential alternative therapeutic agents for Blastocystis hominis.Methods: The parasites were challenged with Ag Nps, Ag Nps + MTZ and MTZ. To assess the efficacy of drugs, counting of viable parasites was done after 1, 2, and 3 hours of adding the drugs.Results: Blastocystis hominis count was reduced by 20.72%, 28.23%, and 18.92% after one hour of adding Ag Nps, Ag Nps + MTZ, and MTZ, respectively. Cysts count was further reduced by 51.49%, 61.61%, and 40.78% after 2 hours and by 71.69%, 79.67%, and 62.65% after 3 hours of adding the drugs in the same order, respectively.Conclusion: There was a statistically significant difference (P< 0.05) in the in vitro growth inhibition of the parasite over the different time intervals when using the tested drugs against the control drug.Keywords: Blastocystis hominis, eosin brilliant cresyl blue stain, metronidazole, silver nanoparticleshttps://www.dovepress.com/in-vitro-effect-of-silver-nanoparticles-on-blastocystis-hominis-peer-reviewed-article-IJNblastocystis hominiseosin brilliant cresyl blue stainmetronidazolesilver nanoparticles. |
| spellingShingle | Younis SA Abououf EA Ali AE Abd elhady SM Wassef RM In vitro Effect of Silver Nanoparticles on Blastocystis hominis International Journal of Nanomedicine blastocystis hominis eosin brilliant cresyl blue stain metronidazole silver nanoparticles. |
| title | In vitro Effect of Silver Nanoparticles on Blastocystis hominis |
| title_full | In vitro Effect of Silver Nanoparticles on Blastocystis hominis |
| title_fullStr | In vitro Effect of Silver Nanoparticles on Blastocystis hominis |
| title_full_unstemmed | In vitro Effect of Silver Nanoparticles on Blastocystis hominis |
| title_short | In vitro Effect of Silver Nanoparticles on Blastocystis hominis |
| title_sort | in vitro effect of silver nanoparticles on blastocystis hominis |
| topic | blastocystis hominis eosin brilliant cresyl blue stain metronidazole silver nanoparticles. |
| url | https://www.dovepress.com/in-vitro-effect-of-silver-nanoparticles-on-blastocystis-hominis-peer-reviewed-article-IJN |
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