Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube
The lumen of the fallopian tube (FT) is lined with columnar epithelium composed of secretory and ciliated cells, both of which are important for reproduction. However, the molecular mechanism regulating cell fate remains controversial. In this study, we established a primary culture system using por...
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MDPI AG
2019-08-01
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| Online Access: | https://www.mdpi.com/2073-4409/8/8/933 |
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| author | Maobi Zhu Tomohiko Iwano Sen Takeda |
| author_facet | Maobi Zhu Tomohiko Iwano Sen Takeda |
| author_sort | Maobi Zhu |
| collection | DOAJ |
| description | The lumen of the fallopian tube (FT) is lined with columnar epithelium composed of secretory and ciliated cells, both of which are important for reproduction. However, the molecular mechanism regulating cell fate remains controversial. In this study, we established a primary culture system using porcine fallopian tube epithelial cells (FTECs) to study the differentiation mechanism. We found that estrogen promoted the differentiation of multi-ciliated cells (MCCs) through estrogen receptor β, following the reduction of DLL1, a ligand of Notch. Meanwhile, epidermal growth factor (EGF), a regulator of epithelial homeostasis and differentiation, suppressed ciliogenesis by the activation of Notch signaling. However, the estrogen pathway did not affect the activation of the EGF pathway. Taken together, the differentiation of MMCs in FT depends on the balance of EGF and estrogen signaling, either of which inhibits or stimulates the Notch signaling pathway respectively. |
| first_indexed | 2024-03-12T19:49:19Z |
| format | Article |
| id | doaj.art-9ccbab16ed7b4f8893a4c3e3963956d4 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:49:19Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-9ccbab16ed7b4f8893a4c3e3963956d42023-08-02T03:20:50ZengMDPI AGCells2073-44092019-08-018893310.3390/cells8080933cells8080933Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian TubeMaobi Zhu0Tomohiko Iwano1Sen Takeda2Department of Anatomy and Cell Biology, Graduate School of Medicine, University of Yamanashi, 1110 Shimo-Kateau, Chuo, Yamanashi 409-3898, JapanDepartment of Anatomy and Cell Biology, Graduate School of Medicine, University of Yamanashi, 1110 Shimo-Kateau, Chuo, Yamanashi 409-3898, JapanDepartment of Anatomy and Cell Biology, Graduate School of Medicine, University of Yamanashi, 1110 Shimo-Kateau, Chuo, Yamanashi 409-3898, JapanThe lumen of the fallopian tube (FT) is lined with columnar epithelium composed of secretory and ciliated cells, both of which are important for reproduction. However, the molecular mechanism regulating cell fate remains controversial. In this study, we established a primary culture system using porcine fallopian tube epithelial cells (FTECs) to study the differentiation mechanism. We found that estrogen promoted the differentiation of multi-ciliated cells (MCCs) through estrogen receptor β, following the reduction of DLL1, a ligand of Notch. Meanwhile, epidermal growth factor (EGF), a regulator of epithelial homeostasis and differentiation, suppressed ciliogenesis by the activation of Notch signaling. However, the estrogen pathway did not affect the activation of the EGF pathway. Taken together, the differentiation of MMCs in FT depends on the balance of EGF and estrogen signaling, either of which inhibits or stimulates the Notch signaling pathway respectively.https://www.mdpi.com/2073-4409/8/8/933motile ciliaERβdifferentiationDLL1high-grade serous ovarian cancer |
| spellingShingle | Maobi Zhu Tomohiko Iwano Sen Takeda Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube Cells motile cilia ERβ differentiation DLL1 high-grade serous ovarian cancer |
| title | Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube |
| title_full | Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube |
| title_fullStr | Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube |
| title_full_unstemmed | Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube |
| title_short | Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube |
| title_sort | estrogen and egfr pathways regulate notch signaling in opposing directions for multi ciliogenesis in the fallopian tube |
| topic | motile cilia ERβ differentiation DLL1 high-grade serous ovarian cancer |
| url | https://www.mdpi.com/2073-4409/8/8/933 |
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