Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis.
<h4>Background</h4>Interleukin-18 (IL-18) and interferon-γ (IFN-γ) are cytokines of crucial role in inflammation and immune reactions. There is a growing evidence supporting important roles for IL-18 and IFN γ in tuberculosis (TB) infection and anti-tuberculosis immunity.<h4>Object...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0244949 |
| _version_ | 1818901586610487296 |
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| author | Noha A Hassuna Mohamed El Feky Aliae A R Mohamed Hussein Manal A Mahmoud Naglaa K Idriss Sayed F Abdelwahab Maggie A Ibrahim |
| author_facet | Noha A Hassuna Mohamed El Feky Aliae A R Mohamed Hussein Manal A Mahmoud Naglaa K Idriss Sayed F Abdelwahab Maggie A Ibrahim |
| author_sort | Noha A Hassuna |
| collection | DOAJ |
| description | <h4>Background</h4>Interleukin-18 (IL-18) and interferon-γ (IFN-γ) are cytokines of crucial role in inflammation and immune reactions. There is a growing evidence supporting important roles for IL-18 and IFN γ in tuberculosis (TB) infection and anti-tuberculosis immunity.<h4>Objective</h4>To evaluate the role of polymorphisms in IL-18-607 and -137 and INF-γ +874 in susceptibility to TB infection among Egyptian patients.<h4>Methods</h4>A case control study was conducted to investigate the polymorphism at IL-18-607, -137 and INF-γ+874 by sequence specific primer-polymerase chain reaction (SSP- PCR) in 105 patients with pulmonary and extra pulmonary tuberculosis and 106 controls.<h4>Results</h4>A significant protective effect against TB was found in homozygous CC genotype at IL-18 -137G/C, in addition to a 7-fold risk with GG and GC genotypes in the recessive model. Apart from a decreased risk with the AC genotype, no association was detected between the susceptibility to TB and different genotypes or alleles at the IL-18 -607A/C site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes with nearly a 2-fold risk of TB infection with the A allele. Regarding haplotype association, the GC haplotype was strongly associated with TB infection compared to other haplotypes.<h4>Conclusion</h4>These findings suggest; for the first time in Egypt; a significant risk to TB infection with SNP at the IL-18-137G/C with no LD with SNP at the IL-18-607 site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes. |
| first_indexed | 2024-12-19T20:22:07Z |
| format | Article |
| id | doaj.art-9ccdc32a71f844b49c6e09c812c6abbe |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T20:22:07Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9ccdc32a71f844b49c6e09c812c6abbe2022-12-21T20:06:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01161e024494910.1371/journal.pone.0244949Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis.Noha A HassunaMohamed El FekyAliae A R Mohamed HusseinManal A MahmoudNaglaa K IdrissSayed F AbdelwahabMaggie A Ibrahim<h4>Background</h4>Interleukin-18 (IL-18) and interferon-γ (IFN-γ) are cytokines of crucial role in inflammation and immune reactions. There is a growing evidence supporting important roles for IL-18 and IFN γ in tuberculosis (TB) infection and anti-tuberculosis immunity.<h4>Objective</h4>To evaluate the role of polymorphisms in IL-18-607 and -137 and INF-γ +874 in susceptibility to TB infection among Egyptian patients.<h4>Methods</h4>A case control study was conducted to investigate the polymorphism at IL-18-607, -137 and INF-γ+874 by sequence specific primer-polymerase chain reaction (SSP- PCR) in 105 patients with pulmonary and extra pulmonary tuberculosis and 106 controls.<h4>Results</h4>A significant protective effect against TB was found in homozygous CC genotype at IL-18 -137G/C, in addition to a 7-fold risk with GG and GC genotypes in the recessive model. Apart from a decreased risk with the AC genotype, no association was detected between the susceptibility to TB and different genotypes or alleles at the IL-18 -607A/C site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes with nearly a 2-fold risk of TB infection with the A allele. Regarding haplotype association, the GC haplotype was strongly associated with TB infection compared to other haplotypes.<h4>Conclusion</h4>These findings suggest; for the first time in Egypt; a significant risk to TB infection with SNP at the IL-18-137G/C with no LD with SNP at the IL-18-607 site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes.https://doi.org/10.1371/journal.pone.0244949 |
| spellingShingle | Noha A Hassuna Mohamed El Feky Aliae A R Mohamed Hussein Manal A Mahmoud Naglaa K Idriss Sayed F Abdelwahab Maggie A Ibrahim Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis. PLoS ONE |
| title | Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis. |
| title_full | Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis. |
| title_fullStr | Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis. |
| title_full_unstemmed | Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis. |
| title_short | Interleukin-18 and interferon-γ single nucleotide polymorphisms in Egyptian patients with tuberculosis. |
| title_sort | interleukin 18 and interferon γ single nucleotide polymorphisms in egyptian patients with tuberculosis |
| url | https://doi.org/10.1371/journal.pone.0244949 |
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