Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development
During animal ontogenesis, body axis patterning is finely regulated by complex interactions among several signaling pathways. Nitric oxide (NO) and retinoic acid (RA) are potent morphogens that play a pivotal role in vertebrate development. Their involvement in axial patterning of the head and phary...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-08-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/58295 |
| _version_ | 1811253318802997248 |
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| author | Filomena Caccavale Giovanni Annona Lucie Subirana Hector Escriva Stephanie Bertrand Salvatore D'Aniello |
| author_facet | Filomena Caccavale Giovanni Annona Lucie Subirana Hector Escriva Stephanie Bertrand Salvatore D'Aniello |
| author_sort | Filomena Caccavale |
| collection | DOAJ |
| description | During animal ontogenesis, body axis patterning is finely regulated by complex interactions among several signaling pathways. Nitric oxide (NO) and retinoic acid (RA) are potent morphogens that play a pivotal role in vertebrate development. Their involvement in axial patterning of the head and pharynx shows conserved features in the chordate phylum. Indeed, in the cephalochordate amphioxus, NO and RA are crucial for the correct development of pharyngeal structures. Here, we demonstrate the functional cooperation between NO and RA that occurs during amphioxus embryogenesis. During neurulation, NO modulates RA production through the transcriptional regulation of Aldh1a.2 that irreversibly converts retinaldehyde into RA. On the other hand, RA directly or indirectly regulates the transcription of Nos genes. This reciprocal regulation of NO and RA pathways is essential for the normal pharyngeal development in amphioxus and it could be conserved in vertebrates. |
| first_indexed | 2024-04-12T16:49:02Z |
| format | Article |
| id | doaj.art-9ccea94247b5433faba2254421563e72 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:49:02Z |
| publishDate | 2021-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9ccea94247b5433faba2254421563e722022-12-22T03:24:27ZengeLife Sciences Publications LtdeLife2050-084X2021-08-011010.7554/eLife.58295Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx developmentFilomena Caccavale0Giovanni Annona1https://orcid.org/0000-0001-7806-6761Lucie Subirana2Hector Escriva3Stephanie Bertrand4Salvatore D'Aniello5https://orcid.org/0000-0001-7294-1465Department of Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn Napoli, Napoli, ItalyDepartment of Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn Napoli, Napoli, ItalySorbonne Université CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, Banyuls-sur-Mer, FranceSorbonne Université CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, Banyuls-sur-Mer, FranceSorbonne Université CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, Banyuls-sur-Mer, FranceDepartment of Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn Napoli, Napoli, ItalyDuring animal ontogenesis, body axis patterning is finely regulated by complex interactions among several signaling pathways. Nitric oxide (NO) and retinoic acid (RA) are potent morphogens that play a pivotal role in vertebrate development. Their involvement in axial patterning of the head and pharynx shows conserved features in the chordate phylum. Indeed, in the cephalochordate amphioxus, NO and RA are crucial for the correct development of pharyngeal structures. Here, we demonstrate the functional cooperation between NO and RA that occurs during amphioxus embryogenesis. During neurulation, NO modulates RA production through the transcriptional regulation of Aldh1a.2 that irreversibly converts retinaldehyde into RA. On the other hand, RA directly or indirectly regulates the transcription of Nos genes. This reciprocal regulation of NO and RA pathways is essential for the normal pharyngeal development in amphioxus and it could be conserved in vertebrates.https://elifesciences.org/articles/58295nitric oxide synthaseRA pathwaychordatedevelopmental biologyevolutionbranchiostoma lanceolatum |
| spellingShingle | Filomena Caccavale Giovanni Annona Lucie Subirana Hector Escriva Stephanie Bertrand Salvatore D'Aniello Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development eLife nitric oxide synthase RA pathway chordate developmental biology evolution branchiostoma lanceolatum |
| title | Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development |
| title_full | Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development |
| title_fullStr | Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development |
| title_full_unstemmed | Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development |
| title_short | Crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development |
| title_sort | crosstalk between nitric oxide and retinoic acid pathways is essential for amphioxus pharynx development |
| topic | nitric oxide synthase RA pathway chordate developmental biology evolution branchiostoma lanceolatum |
| url | https://elifesciences.org/articles/58295 |
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