Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China
This work investigated the genetic relationship among Stenotrophomonas maltophilia strains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-09-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2023-032 |
| _version_ | 1797674850203992064 |
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| author | Shao Lanjun Liu Xiaojing Liu Yi Shen Jie Liu Ruishan Chen Pingnan |
| author_facet | Shao Lanjun Liu Xiaojing Liu Yi Shen Jie Liu Ruishan Chen Pingnan |
| author_sort | Shao Lanjun |
| collection | DOAJ |
| description | This work investigated the genetic relationship among Stenotrophomonas maltophilia strains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the isolates were identified with MALDI-TOF/MS. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 platform (Illumina, Inc., USA). The antimicrobial resistance genes were detected using CGE services. The phylogenetic analysis of S. maltophilia strains was performed by Roary and MEGA X. In total, 24 S. maltophilia isolates were isolated. The results of resistome analysis showed all S. maltophilia strains carrying blaL1 gene, which was the only β-lactamase genotype. In addition, the aminoglycoside resistance genes aac(6′)-Iz and aph(3′)-IIc were found. The phylogenetic tree indicated the clonal diversity of S. maltophilia in these two regions and the clonal relatedness of the strains from these regions. This study first investigated the dissemination and characterization of S. maltophilia isolates from dairy cows in northeast China and provided evidence of the potential transmission between two provinces. Furthermore, it indicated blaL1 was the most prevalent genotype of β-lactamase in these regions. |
| first_indexed | 2024-03-11T22:05:04Z |
| format | Article |
| id | doaj.art-9ccf5f2975f24130b578e855b611165e |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-03-11T22:05:04Z |
| publishDate | 2023-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-9ccf5f2975f24130b578e855b611165e2023-09-25T06:07:49ZengSciendoPolish Journal of Microbiology2544-46462023-09-0172331932310.33073/pjm-2023-032Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast ChinaShao Lanjun0Liu Xiaojing1Liu Yi2Shen Jie3Liu Ruishan4Chen Pingnan5Health Management Center, The Third Affiliated Hospital of Zhejiang Traditional Chinese Medical University, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Laboratory Medicine, Pingguo People's Hospital, Baise, ChinaThis work investigated the genetic relationship among Stenotrophomonas maltophilia strains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the isolates were identified with MALDI-TOF/MS. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 platform (Illumina, Inc., USA). The antimicrobial resistance genes were detected using CGE services. The phylogenetic analysis of S. maltophilia strains was performed by Roary and MEGA X. In total, 24 S. maltophilia isolates were isolated. The results of resistome analysis showed all S. maltophilia strains carrying blaL1 gene, which was the only β-lactamase genotype. In addition, the aminoglycoside resistance genes aac(6′)-Iz and aph(3′)-IIc were found. The phylogenetic tree indicated the clonal diversity of S. maltophilia in these two regions and the clonal relatedness of the strains from these regions. This study first investigated the dissemination and characterization of S. maltophilia isolates from dairy cows in northeast China and provided evidence of the potential transmission between two provinces. Furthermore, it indicated blaL1 was the most prevalent genotype of β-lactamase in these regions.https://doi.org/10.33073/pjm-2023-032stenotrophomonas maltophiliablal1 genewhole-genome sequencingphylogenetic analysis |
| spellingShingle | Shao Lanjun Liu Xiaojing Liu Yi Shen Jie Liu Ruishan Chen Pingnan Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China Polish Journal of Microbiology stenotrophomonas maltophilia blal1 gene whole-genome sequencing phylogenetic analysis |
| title | Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China |
| title_full | Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China |
| title_fullStr | Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China |
| title_full_unstemmed | Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China |
| title_short | Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China |
| title_sort | dissemination and characterization of stenotrophomonas maltophilia isolates from dairy cows in northeast china |
| topic | stenotrophomonas maltophilia blal1 gene whole-genome sequencing phylogenetic analysis |
| url | https://doi.org/10.33073/pjm-2023-032 |
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