Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum)
Objective of this study was to isolate, sequence and analyze variations in candidate genes involved in nitrogen (N) metabolism in potato (Solanum tuberosum L.) for future breeding application. Two contrasting potato varieties, viz. Kufri Gaurav (N efficient) and Kufri Jyoti (N inefficient) were use...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2018-05-01
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| Series: | The Indian Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/80072 |
| _version_ | 1827893046265511936 |
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| author | JAGESH KUMAR TIWARI SAPNA DEVI NILOFER ALI VIJAY K DUA RAJESH K SINGH SWARUP K CHAKRABARTI |
| author_facet | JAGESH KUMAR TIWARI SAPNA DEVI NILOFER ALI VIJAY K DUA RAJESH K SINGH SWARUP K CHAKRABARTI |
| author_sort | JAGESH KUMAR TIWARI |
| collection | DOAJ |
| description |
Objective of this study was to isolate, sequence and analyze variations in candidate genes involved in nitrogen (N) metabolism in potato (Solanum tuberosum L.) for future breeding application. Two contrasting potato varieties, viz. Kufri Gaurav (N efficient) and Kufri Jyoti (N inefficient) were used. Of the total 17 PCR primers tested for N metabolism genes in the both varieties and only 12 distinct bands were cloned and sequenced, which were amplified by six primers of five genes (nitrate transporter-NRT, ammonium transporter-AMT, nitrate reductase-NR, nitrite reductase-NIR and asparagines synthetase-AS). Following sequence analysis, non-redundant sequences with uninterrupted open reading frames of 12 ‘N-homologous genes’ were identified to the known N metabolic pathway genes. Thus, the identified 12 N-homologous genes may serve as an important genomic resource for novel gene/marker discovery and would be useful for marker-assisted selection (MAS) in potato with better nitrogen use efficiency (NUE).
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| first_indexed | 2024-03-12T21:49:24Z |
| format | Article |
| id | doaj.art-9ccf90d9407644b284d7e2ff42d1799c |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-03-12T21:49:24Z |
| publishDate | 2018-05-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-9ccf90d9407644b284d7e2ff42d1799c2023-07-26T06:51:19ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192018-05-0188510.56093/ijas.v88i5.80072Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum)JAGESH KUMAR TIWARI0SAPNA DEVI1NILOFER ALI2VIJAY K DUA3RAJESH K SINGH4SWARUP K CHAKRABARTI5Scientist (Senior Scale), ICAR-Central Potato Research Institute, Shimla, Himachal PradeshJRF, ICAR-Central Potato Research Institute, Shimla, Himachal PradeshJRF, ICAR-Central Potato Research Institute, Shimla, Himachal PradeshHead and Principal Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal PradeshHead and Principal Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal PradeshDirector, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh Objective of this study was to isolate, sequence and analyze variations in candidate genes involved in nitrogen (N) metabolism in potato (Solanum tuberosum L.) for future breeding application. Two contrasting potato varieties, viz. Kufri Gaurav (N efficient) and Kufri Jyoti (N inefficient) were used. Of the total 17 PCR primers tested for N metabolism genes in the both varieties and only 12 distinct bands were cloned and sequenced, which were amplified by six primers of five genes (nitrate transporter-NRT, ammonium transporter-AMT, nitrate reductase-NR, nitrite reductase-NIR and asparagines synthetase-AS). Following sequence analysis, non-redundant sequences with uninterrupted open reading frames of 12 ‘N-homologous genes’ were identified to the known N metabolic pathway genes. Thus, the identified 12 N-homologous genes may serve as an important genomic resource for novel gene/marker discovery and would be useful for marker-assisted selection (MAS) in potato with better nitrogen use efficiency (NUE). https://epubs.icar.org.in/index.php/IJAgS/article/view/80072CloningNitrogen metabolism genesNitrogen use efficiencyPotatoSequencing |
| spellingShingle | JAGESH KUMAR TIWARI SAPNA DEVI NILOFER ALI VIJAY K DUA RAJESH K SINGH SWARUP K CHAKRABARTI Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum) The Indian Journal of Agricultural Sciences Cloning Nitrogen metabolism genes Nitrogen use efficiency Potato Sequencing |
| title | Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum) |
| title_full | Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum) |
| title_fullStr | Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum) |
| title_full_unstemmed | Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum) |
| title_short | Cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato (Solanum tuberosum) |
| title_sort | cloning and sequence variation analysis of candidate genes involved in nitrogen metabolism in potato solanum tuberosum |
| topic | Cloning Nitrogen metabolism genes Nitrogen use efficiency Potato Sequencing |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/80072 |
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