SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments.
LITAF is a small cellular protein with an unknown function. The C-terminus of LITAF contains a highly conserved domain termed the SIMPLE-like domain (SLD), while the N-terminus contains two PPXY motifs that mediate protein-protein interactions with WW-domain containing proteins. LITAF also harbors t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-02-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3033906?pdf=render |
| _version_ | 1818131592581742592 |
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| author | Heather E Eaton Guillaume Desrochers Samuel B Drory Julie Metcalf Annie Angers Craig R Brunetti |
| author_facet | Heather E Eaton Guillaume Desrochers Samuel B Drory Julie Metcalf Annie Angers Craig R Brunetti |
| author_sort | Heather E Eaton |
| collection | DOAJ |
| description | LITAF is a small cellular protein with an unknown function. The C-terminus of LITAF contains a highly conserved domain termed the SIMPLE-like domain (SLD), while the N-terminus contains two PPXY motifs that mediate protein-protein interactions with WW-domain containing proteins. LITAF also harbors two endosome/lysosome targeting sequences at its C-terminus, but there has been conflicting reports regarding its intracellular localization. Here, we demonstrate that LITAF is localized to the late endosome/lysosomal compartment in a variety of cell lines. We also show that Itch, a WW-domain containing protein, and LITAF strongly interact and that this interaction depends on the two PPXY motifs in the N-terminus of LITAF. Interestingly, co-expression of LITAF with Itch induces major changes in Itch intracellular localization, bringing Itch from the trans-Golgi network to lysosomes. We show that this re-localization is dependent upon the interaction with the PPXY sequences of LITAF, since disruption of these binding motifs completely abrogates Itch re-localization. |
| first_indexed | 2024-12-11T08:23:23Z |
| format | Article |
| id | doaj.art-9cd2403a354341a78081352b86d30b84 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:23:23Z |
| publishDate | 2011-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9cd2403a354341a78081352b86d30b842022-12-22T01:14:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-02-0162e1687310.1371/journal.pone.0016873SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments.Heather E EatonGuillaume DesrochersSamuel B DroryJulie MetcalfAnnie AngersCraig R BrunettiLITAF is a small cellular protein with an unknown function. The C-terminus of LITAF contains a highly conserved domain termed the SIMPLE-like domain (SLD), while the N-terminus contains two PPXY motifs that mediate protein-protein interactions with WW-domain containing proteins. LITAF also harbors two endosome/lysosome targeting sequences at its C-terminus, but there has been conflicting reports regarding its intracellular localization. Here, we demonstrate that LITAF is localized to the late endosome/lysosomal compartment in a variety of cell lines. We also show that Itch, a WW-domain containing protein, and LITAF strongly interact and that this interaction depends on the two PPXY motifs in the N-terminus of LITAF. Interestingly, co-expression of LITAF with Itch induces major changes in Itch intracellular localization, bringing Itch from the trans-Golgi network to lysosomes. We show that this re-localization is dependent upon the interaction with the PPXY sequences of LITAF, since disruption of these binding motifs completely abrogates Itch re-localization.http://europepmc.org/articles/PMC3033906?pdf=render |
| spellingShingle | Heather E Eaton Guillaume Desrochers Samuel B Drory Julie Metcalf Annie Angers Craig R Brunetti SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments. PLoS ONE |
| title | SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments. |
| title_full | SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments. |
| title_fullStr | SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments. |
| title_full_unstemmed | SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments. |
| title_short | SIMPLE/LITAF expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments. |
| title_sort | simple litaf expression induces the translocation of the ubiquitin ligase itch towards the lysosomal compartments |
| url | http://europepmc.org/articles/PMC3033906?pdf=render |
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