Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats
Perirenal adipose tissue (PRAT) surrounding the kidney is emerging as a player and novel independent risk factor in diabetic kidney disease (DKD); DKD is a complication of diabetes and is a major cause of increased cardiovascular (CV) risk and CV mortality in affected patients. We determined the eff...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/17008 |
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| author | Elena Vega-Martín Daniel González-Moreno Marta Sanz-Gómez Ana Karen Guzmán-Aguayo Francisco Javier Manzano-Lista Angela Schulz Isabel Aránguez Reinhold Kreutz María S. Fernández-Alfonso |
| author_facet | Elena Vega-Martín Daniel González-Moreno Marta Sanz-Gómez Ana Karen Guzmán-Aguayo Francisco Javier Manzano-Lista Angela Schulz Isabel Aránguez Reinhold Kreutz María S. Fernández-Alfonso |
| author_sort | Elena Vega-Martín |
| collection | DOAJ |
| description | Perirenal adipose tissue (PRAT) surrounding the kidney is emerging as a player and novel independent risk factor in diabetic kidney disease (DKD); DKD is a complication of diabetes and is a major cause of increased cardiovascular (CV) risk and CV mortality in affected patients. We determined the effect of diabetes induction on (i) kidney and CV damage and (ii) on the expression of proinflammatory and profibrotic factors in both the PRAT and the mesenteric adipose tissue (MAT) of Munich Wistar Frömter (MWF) rats. The 16-week-old male MWF rats (n = 10 rats/group) were fed standard chow (MWF-C) or a high-fat/high-sucrose diet for 6 weeks together with low-dose streptozotocin (15 mg/kg i.p.) at the start of dietary exposure (MWF-D). Phenotyping was performed at the end of treatment through determining water intake, urine excretion, and oral glucose tolerance; use of the homeostatic model assessment–insulin resistance index (HOMA-IR) evidenced the development of overt diabetes manifestation in MWF-D rats. The kidney damage markers <i>Kim-1</i> and <i>Ngal</i> were significantly higher in MWF-D rats, as were the amounts of PRAT and MAT. A diabetes-induced upregulation in <i>IL-1</i>, <i>IL-6</i>, <i>Tnf-α</i>, and <i>Tgf-β</i> was observed in both the PRAT and the MAT. <i>Col1A1</i> was increased in the PRAT but not in the MAT of MWF-D, whereas <i>IL-10</i> was lower and higher in the PRAT and the MAT, respectively. Urinary albumin excretion and blood pressure were not further increased by diabetes induction, while heart weight was higher in the MWF-D. In conclusion, our results show a proinflammatory and profibrotic in vivo environment in PRAT induced by diabetes which might be associated with kidney damage progression in the MWF strain. |
| first_indexed | 2024-03-09T01:49:06Z |
| format | Article |
| id | doaj.art-9cd4db7994a343a9bb48d26287f1b955 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:49:06Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9cd4db7994a343a9bb48d26287f1b9552023-12-08T15:18:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231700810.3390/ijms242317008Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter RatsElena Vega-Martín0Daniel González-Moreno1Marta Sanz-Gómez2Ana Karen Guzmán-Aguayo3Francisco Javier Manzano-Lista4Angela Schulz5Isabel Aránguez6Reinhold Kreutz7María S. Fernández-Alfonso8Instituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainInstituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainInstituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainInstituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainInstituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin, 10117 Berlin, GermanyInstituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin, 10117 Berlin, GermanyInstituto Pluridisciplinar and Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, SpainPerirenal adipose tissue (PRAT) surrounding the kidney is emerging as a player and novel independent risk factor in diabetic kidney disease (DKD); DKD is a complication of diabetes and is a major cause of increased cardiovascular (CV) risk and CV mortality in affected patients. We determined the effect of diabetes induction on (i) kidney and CV damage and (ii) on the expression of proinflammatory and profibrotic factors in both the PRAT and the mesenteric adipose tissue (MAT) of Munich Wistar Frömter (MWF) rats. The 16-week-old male MWF rats (n = 10 rats/group) were fed standard chow (MWF-C) or a high-fat/high-sucrose diet for 6 weeks together with low-dose streptozotocin (15 mg/kg i.p.) at the start of dietary exposure (MWF-D). Phenotyping was performed at the end of treatment through determining water intake, urine excretion, and oral glucose tolerance; use of the homeostatic model assessment–insulin resistance index (HOMA-IR) evidenced the development of overt diabetes manifestation in MWF-D rats. The kidney damage markers <i>Kim-1</i> and <i>Ngal</i> were significantly higher in MWF-D rats, as were the amounts of PRAT and MAT. A diabetes-induced upregulation in <i>IL-1</i>, <i>IL-6</i>, <i>Tnf-α</i>, and <i>Tgf-β</i> was observed in both the PRAT and the MAT. <i>Col1A1</i> was increased in the PRAT but not in the MAT of MWF-D, whereas <i>IL-10</i> was lower and higher in the PRAT and the MAT, respectively. Urinary albumin excretion and blood pressure were not further increased by diabetes induction, while heart weight was higher in the MWF-D. In conclusion, our results show a proinflammatory and profibrotic in vivo environment in PRAT induced by diabetes which might be associated with kidney damage progression in the MWF strain.https://www.mdpi.com/1422-0067/24/23/17008chronic kidney diseasediabetesperirenal adipose tissuemesenteric adipose tissueexperimental modelMunich Wistar Frömter rat |
| spellingShingle | Elena Vega-Martín Daniel González-Moreno Marta Sanz-Gómez Ana Karen Guzmán-Aguayo Francisco Javier Manzano-Lista Angela Schulz Isabel Aránguez Reinhold Kreutz María S. Fernández-Alfonso Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats International Journal of Molecular Sciences chronic kidney disease diabetes perirenal adipose tissue mesenteric adipose tissue experimental model Munich Wistar Frömter rat |
| title | Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats |
| title_full | Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats |
| title_fullStr | Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats |
| title_full_unstemmed | Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats |
| title_short | Upregulation in Inflammation and Collagen Expression in Perirenal but Not in Mesenteric Adipose Tissue from Diabetic Munich Wistar Frömter Rats |
| title_sort | upregulation in inflammation and collagen expression in perirenal but not in mesenteric adipose tissue from diabetic munich wistar fromter rats |
| topic | chronic kidney disease diabetes perirenal adipose tissue mesenteric adipose tissue experimental model Munich Wistar Frömter rat |
| url | https://www.mdpi.com/1422-0067/24/23/17008 |
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