The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen
<i>Mycoplasma bovis</i> is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial...
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MDPI AG
2020-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/5/837 |
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| author | Ana García-Galán Ángel Gómez-Martín Esther Bataller Jesús Gomis Antonio Sánchez Joaquín Gadea Luis Alberto Vieira Empar García-Roselló Christian De la Fe |
| author_facet | Ana García-Galán Ángel Gómez-Martín Esther Bataller Jesús Gomis Antonio Sánchez Joaquín Gadea Luis Alberto Vieira Empar García-Roselló Christian De la Fe |
| author_sort | Ana García-Galán |
| collection | DOAJ |
| description | <i>Mycoplasma bovis</i> is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two <i>M. bovis</i> mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain <i>M. bovis-</i>free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of <i>M. bovis</i> through AI technologies. The viability of <i>M. bovis</i> (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a <i>Lactobacillus</i> spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 10<sup>6</sup> colony forming units (CFU)/mL or 3.24 × 10<sup>8</sup> CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of <i>M. bovis</i>, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (<i>p <</i> 0.05). Our results may support new strategies for reducing the risk of <i>M. bovis</i> transmission through AI. |
| first_indexed | 2024-03-10T19:51:45Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T19:51:45Z |
| publishDate | 2020-05-01 |
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| series | Animals |
| spelling | doaj.art-9cd828067ac64b83a04b8750be8878c32023-11-20T00:18:50ZengMDPI AGAnimals2076-26152020-05-0110583710.3390/ani10050837The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine SemenAna García-Galán0Ángel Gómez-Martín1Esther Bataller2Jesús Gomis3Antonio Sánchez4Joaquín Gadea5Luis Alberto Vieira6Empar García-Roselló7Christian De la Fe8Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainRuminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities, 46113 Valencia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities, 46113 Valencia, SpainRuminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainPhysiology of Reproduction Research Group, Department of Physiology, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainPhysiology of Reproduction Research Group, Department of Physiology, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBio) Research Group, Department of Animal Health and Public Health, Faculty of Veterinary Sciences, University CEU Cardenal Herrera of Valencia, CEU Universities, 46113 Valencia, SpainRuminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain<i>Mycoplasma bovis</i> is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two <i>M. bovis</i> mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain <i>M. bovis-</i>free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of <i>M. bovis</i> through AI technologies. The viability of <i>M. bovis</i> (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a <i>Lactobacillus</i> spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 10<sup>6</sup> colony forming units (CFU)/mL or 3.24 × 10<sup>8</sup> CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of <i>M. bovis</i>, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (<i>p <</i> 0.05). Our results may support new strategies for reducing the risk of <i>M. bovis</i> transmission through AI.https://www.mdpi.com/2076-2615/10/5/837In vitroviability<i>Mycoplasma bovis</i>diluted bull semenenrofloxacindoxycycline |
| spellingShingle | Ana García-Galán Ángel Gómez-Martín Esther Bataller Jesús Gomis Antonio Sánchez Joaquín Gadea Luis Alberto Vieira Empar García-Roselló Christian De la Fe The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen Animals In vitro viability <i>Mycoplasma bovis</i> diluted bull semen enrofloxacin doxycycline |
| title | The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen |
| title_full | The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen |
| title_fullStr | The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen |
| title_full_unstemmed | The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen |
| title_short | The Addition of <i>Lactobacillus</i> spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of <i>Mycoplasma bovis</i> in Diluted Bovine Semen |
| title_sort | addition of i lactobacillus i spp enrofloxacin or doxycycline negatively affects the viability of i mycoplasma bovis i in diluted bovine semen |
| topic | In vitro viability <i>Mycoplasma bovis</i> diluted bull semen enrofloxacin doxycycline |
| url | https://www.mdpi.com/2076-2615/10/5/837 |
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