Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G
Hepatocellular carcinoma (HCC) has the highest incidence and mortality in the Asian population, and race is an independent risk factor affecting survival time in liver cancer. Micro RNAs (miRNAs) are remarkably dysregulated in HCC and closely associated with HCC prognosis. Recent studies show that g...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/16/1/129 |
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| author | Zhouxiang Liao Qi Zhang Lichao Yang Hui Li Wanling Mo Zhenyu Song Xuejing Huang Sha Wen Xiaojing Cheng Min He |
| author_facet | Zhouxiang Liao Qi Zhang Lichao Yang Hui Li Wanling Mo Zhenyu Song Xuejing Huang Sha Wen Xiaojing Cheng Min He |
| author_sort | Zhouxiang Liao |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) has the highest incidence and mortality in the Asian population, and race is an independent risk factor affecting survival time in liver cancer. Micro RNAs (miRNAs) are remarkably dysregulated in HCC and closely associated with HCC prognosis. Recent studies show that genetic variability between ethnic groups may result in differences in the specificity of HCC miRNA biomarkers. Here, we reveal a high expression level of hsa-miR-100-5p, an HCC prognosis-related miRNA, which improves HCC prognosis in the Asian Population with Polo-like kinase 1 (PLK1) variant rs27770A>G. In this study, we discovered that hsa-miR-100-5p was downregulated in various HCC cell lines. While mimics transient transfection and mouse liver cancer model confirmed the interaction between hsa-miR-100-5p and <i>PLK1</i>, a stratified analysis based on the Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) data suggest both low hsa-miR-100-5p expression level and high <i>PLK1</i> expression level associated with poor HCC prognosis, especially in the Asian population. According to the 1000 Genomes Project database, the SNP rs27770 located in 3′UTR of <i>PLK1</i> had a significantly higher G allele frequency in the East Asian population. Bioinformatics analysis suggested that rs27770 A>G affects <i>PLK1</i> mRNA secondary structure and alters the hsa-miR-100-5p/<i>PLK1</i> interaction by forming an additional seedless binding site. This racial variation caused <i>PLK1</i> to be more vulnerable to hsa-miR-100-5p inhibition, resulting in hsa-miR-100-5p being more favorable for HCC prognosis in the Asian population. |
| first_indexed | 2024-03-08T15:10:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-08T15:10:35Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Cancers |
| spelling | doaj.art-9cdb15154afd4ee9a7af1a5c0fe154d42024-01-10T14:52:47ZengMDPI AGCancers2072-66942023-12-0116112910.3390/cancers16010129Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>GZhouxiang Liao0Qi Zhang1Lichao Yang2Hui Li3Wanling Mo4Zhenyu Song5Xuejing Huang6Sha Wen7Xiaojing Cheng8Min He9School of Public Health, Guangxi Medical University, Nanning 530021, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning 530021, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning 530021, ChinaSchool of Public Health, Guangxi Medical University, Nanning 530021, ChinaSchool of Public Health, Guangxi Medical University, Nanning 530021, ChinaSchool of Public Health, Guangxi Medical University, Nanning 530021, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning 530021, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning 530021, ChinaSchool of Public Health, Guangxi Medical University, Nanning 530021, ChinaSchool of Public Health, Guangxi Medical University, Nanning 530021, ChinaHepatocellular carcinoma (HCC) has the highest incidence and mortality in the Asian population, and race is an independent risk factor affecting survival time in liver cancer. Micro RNAs (miRNAs) are remarkably dysregulated in HCC and closely associated with HCC prognosis. Recent studies show that genetic variability between ethnic groups may result in differences in the specificity of HCC miRNA biomarkers. Here, we reveal a high expression level of hsa-miR-100-5p, an HCC prognosis-related miRNA, which improves HCC prognosis in the Asian Population with Polo-like kinase 1 (PLK1) variant rs27770A>G. In this study, we discovered that hsa-miR-100-5p was downregulated in various HCC cell lines. While mimics transient transfection and mouse liver cancer model confirmed the interaction between hsa-miR-100-5p and <i>PLK1</i>, a stratified analysis based on the Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) data suggest both low hsa-miR-100-5p expression level and high <i>PLK1</i> expression level associated with poor HCC prognosis, especially in the Asian population. According to the 1000 Genomes Project database, the SNP rs27770 located in 3′UTR of <i>PLK1</i> had a significantly higher G allele frequency in the East Asian population. Bioinformatics analysis suggested that rs27770 A>G affects <i>PLK1</i> mRNA secondary structure and alters the hsa-miR-100-5p/<i>PLK1</i> interaction by forming an additional seedless binding site. This racial variation caused <i>PLK1</i> to be more vulnerable to hsa-miR-100-5p inhibition, resulting in hsa-miR-100-5p being more favorable for HCC prognosis in the Asian population.https://www.mdpi.com/2072-6694/16/1/129hepatocellular carcinomamiRNAsprognosishsa-miR-100-5p<i>PLK1</i>rs27770 |
| spellingShingle | Zhouxiang Liao Qi Zhang Lichao Yang Hui Li Wanling Mo Zhenyu Song Xuejing Huang Sha Wen Xiaojing Cheng Min He Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G Cancers hepatocellular carcinoma miRNAs prognosis hsa-miR-100-5p <i>PLK1</i> rs27770 |
| title | Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G |
| title_full | Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G |
| title_fullStr | Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G |
| title_full_unstemmed | Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G |
| title_short | Increased hsa-miR-100-5p Expression Improves Hepatocellular Carcinoma Prognosis in the Asian Population with <i>PLK1</i> Variant rs27770A>G |
| title_sort | increased hsa mir 100 5p expression improves hepatocellular carcinoma prognosis in the asian population with i plk1 i variant rs27770a g |
| topic | hepatocellular carcinoma miRNAs prognosis hsa-miR-100-5p <i>PLK1</i> rs27770 |
| url | https://www.mdpi.com/2072-6694/16/1/129 |
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