L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7
ABSTRACTThe pathogenicity of Escherichia coli (E. coli) O157:H7 is predominantly associated with Shiga toxin 2 (Stx2) that poses a huge threat to human and animal intestinal health. Production of Stx2 requires expression of stx2 gene, which is located in the genome of lambdoid Stx2 prophage. Growing...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2023.2221778 |
| _version_ | 1827303746127790080 |
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| author | Jie Hu Yifan Wu Xingjian Zhou Luyuan Kang Shiyi Zhang Yisi Liu Yu Pi Xilong Li Junjun Wang Dandan Han |
| author_facet | Jie Hu Yifan Wu Xingjian Zhou Luyuan Kang Shiyi Zhang Yisi Liu Yu Pi Xilong Li Junjun Wang Dandan Han |
| author_sort | Jie Hu |
| collection | DOAJ |
| description | ABSTRACTThe pathogenicity of Escherichia coli (E. coli) O157:H7 is predominantly associated with Shiga toxin 2 (Stx2) that poses a huge threat to human and animal intestinal health. Production of Stx2 requires expression of stx2 gene, which is located in the genome of lambdoid Stx2 prophage. Growing evidence has implicated that many commonly consumed foods participate in the regulation of prophage induction. In this study, we aimed to explore whether specific dietary functional sugars could inhibit Stx2 prophage induction in E. coli O157:H7, thereby preventing Stx2 production and promoting intestinal health. We demonstrated that Stx2 prophage induction in E. coli O157:H7 was strongly inhibited by L-arabinose both in vitro and in a mouse model. Mechanistically, L-arabinose at doses of 9, 12, or 15 mM diminished RecA protein levels, a master mediator of the SOS response, contributing to reduced Stx2-converting phage induction. L-Arabinose inhibited quorum sensing and oxidative stress response, which are known as positive regulators of the SOS response and subsequent Stx2 phage production. Furthermore, L-arabinose impaired E. coli O157:H7 arginine transport and metabolism that were involved in producing Stx2 phage. Collectively, our results suggest that L-arabinose may be exploited as a novel Stx2 prophage induction inhibitor against E. coli O157:H7 infection. |
| first_indexed | 2024-03-11T14:20:34Z |
| format | Article |
| id | doaj.art-9cdd083a941642a8a3c9c75dcca1bab0 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-24T17:07:31Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-9cdd083a941642a8a3c9c75dcca1bab02024-03-28T22:38:20ZengTaylor & Francis GroupGut Microbes1949-09761949-09842023-12-0115110.1080/19490976.2023.2221778L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7Jie Hu0Yifan Wu1Xingjian Zhou2Luyuan Kang3Shiyi Zhang4Yisi Liu5Yu Pi6Xilong Li7Junjun Wang8Dandan Han9State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaDepartment of Animal Sciences, Wageningen University & Research, Wageningen, NetherlandsState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaABSTRACTThe pathogenicity of Escherichia coli (E. coli) O157:H7 is predominantly associated with Shiga toxin 2 (Stx2) that poses a huge threat to human and animal intestinal health. Production of Stx2 requires expression of stx2 gene, which is located in the genome of lambdoid Stx2 prophage. Growing evidence has implicated that many commonly consumed foods participate in the regulation of prophage induction. In this study, we aimed to explore whether specific dietary functional sugars could inhibit Stx2 prophage induction in E. coli O157:H7, thereby preventing Stx2 production and promoting intestinal health. We demonstrated that Stx2 prophage induction in E. coli O157:H7 was strongly inhibited by L-arabinose both in vitro and in a mouse model. Mechanistically, L-arabinose at doses of 9, 12, or 15 mM diminished RecA protein levels, a master mediator of the SOS response, contributing to reduced Stx2-converting phage induction. L-Arabinose inhibited quorum sensing and oxidative stress response, which are known as positive regulators of the SOS response and subsequent Stx2 phage production. Furthermore, L-arabinose impaired E. coli O157:H7 arginine transport and metabolism that were involved in producing Stx2 phage. Collectively, our results suggest that L-arabinose may be exploited as a novel Stx2 prophage induction inhibitor against E. coli O157:H7 infection.https://www.tandfonline.com/doi/10.1080/19490976.2023.2221778L-ArabinoseEscherichia coli O157:H7Shiga toxin type 2 phagearginine transport and metabolismintestinal health |
| spellingShingle | Jie Hu Yifan Wu Xingjian Zhou Luyuan Kang Shiyi Zhang Yisi Liu Yu Pi Xilong Li Junjun Wang Dandan Han L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7 Gut Microbes L-Arabinose Escherichia coli O157:H7 Shiga toxin type 2 phage arginine transport and metabolism intestinal health |
| title | L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7 |
| title_full | L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7 |
| title_fullStr | L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7 |
| title_full_unstemmed | L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7 |
| title_short | L-Arabinose inhibits Shiga toxin type 2-converting bacteriophage induction in Escherichia coli O157:H7 |
| title_sort | l arabinose inhibits shiga toxin type 2 converting bacteriophage induction in escherichia coli o157 h7 |
| topic | L-Arabinose Escherichia coli O157:H7 Shiga toxin type 2 phage arginine transport and metabolism intestinal health |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2023.2221778 |
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