Aging-associated prostate smooth muscle hypercontractility in rats
Abstract Benign prostatic hyperplasia (BPH) is a multifactorial disease, highly associated with aging and characterized by increased prostate smooth muscle (PSM) contractility. Animal models have been employed to explore the aging-associated PSM hypercontractility; however, studies have focused in o...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-01-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100860&tlng=en |
| _version_ | 1828068196574298112 |
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| author | Tabatha Danielle da Silva Lopes Renan Bazuco Fritoli Fabio Henrique da Silva Fabiano Beraldi Calmasini |
| author_facet | Tabatha Danielle da Silva Lopes Renan Bazuco Fritoli Fabio Henrique da Silva Fabiano Beraldi Calmasini |
| author_sort | Tabatha Danielle da Silva Lopes |
| collection | DOAJ |
| description | Abstract Benign prostatic hyperplasia (BPH) is a multifactorial disease, highly associated with aging and characterized by increased prostate smooth muscle (PSM) contractility. Animal models have been employed to explore the aging-associated PSM hypercontractility; however, studies have focused in old animals, neglecting the initial alterations in early ages. The determination of prostatic dysfunctions onset is crucial to understand the BPH pathophysiology and to propose new BPH treatments. Considering that PSM contractility in 10-month-old rats has already been explored, the aim of the present study was to characterize the PSM contractility in younger rats. Male Wistar control (3.5-month-old), 6- and 8-month-old rats were used. Concentration-response curves to phenylephrine and electrical-field stimulation (EFS) were conducted in prostate from all groups. For the first time, we showed that 6- and 8-month-old rats exhibit PSM hypercontractility. The increased prostate contractility to phenylephrine starts around at 6-month-old, worsening during the aging. The 8-month-old rats exhibited hypercontractility to phenylephrine and EFS compared to the control and 6-month-old groups. Reduced phenylephrine potency was observed in 8-month-old rats, indicating an increased age-dependent prostate sensibility to this agonist. Collectively, our findings support the use of 6- and 8-month-old aged rats as new models to explore prostate hypercontractility in BPH. |
| first_indexed | 2024-04-10T23:59:08Z |
| format | Article |
| id | doaj.art-9cdda0768a9d45f089446f4eb054d37a |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-10T23:59:08Z |
| publishDate | 2023-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-9cdda0768a9d45f089446f4eb054d37a2023-01-10T07:36:22ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-01-015810.1590/s2175-97902022e21063Aging-associated prostate smooth muscle hypercontractility in ratsTabatha Danielle da Silva LopesRenan Bazuco FritoliFabio Henrique da SilvaFabiano Beraldi Calmasinihttps://orcid.org/0000-0002-7790-2550Abstract Benign prostatic hyperplasia (BPH) is a multifactorial disease, highly associated with aging and characterized by increased prostate smooth muscle (PSM) contractility. Animal models have been employed to explore the aging-associated PSM hypercontractility; however, studies have focused in old animals, neglecting the initial alterations in early ages. The determination of prostatic dysfunctions onset is crucial to understand the BPH pathophysiology and to propose new BPH treatments. Considering that PSM contractility in 10-month-old rats has already been explored, the aim of the present study was to characterize the PSM contractility in younger rats. Male Wistar control (3.5-month-old), 6- and 8-month-old rats were used. Concentration-response curves to phenylephrine and electrical-field stimulation (EFS) were conducted in prostate from all groups. For the first time, we showed that 6- and 8-month-old rats exhibit PSM hypercontractility. The increased prostate contractility to phenylephrine starts around at 6-month-old, worsening during the aging. The 8-month-old rats exhibited hypercontractility to phenylephrine and EFS compared to the control and 6-month-old groups. Reduced phenylephrine potency was observed in 8-month-old rats, indicating an increased age-dependent prostate sensibility to this agonist. Collectively, our findings support the use of 6- and 8-month-old aged rats as new models to explore prostate hypercontractility in BPH.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100860&tlng=enBenign prostatic hyperplasiaLower urinary tract symptomsPhenylephrineElectrical-field stimulationSmooth muscle |
| spellingShingle | Tabatha Danielle da Silva Lopes Renan Bazuco Fritoli Fabio Henrique da Silva Fabiano Beraldi Calmasini Aging-associated prostate smooth muscle hypercontractility in rats Brazilian Journal of Pharmaceutical Sciences Benign prostatic hyperplasia Lower urinary tract symptoms Phenylephrine Electrical-field stimulation Smooth muscle |
| title | Aging-associated prostate smooth muscle hypercontractility in rats |
| title_full | Aging-associated prostate smooth muscle hypercontractility in rats |
| title_fullStr | Aging-associated prostate smooth muscle hypercontractility in rats |
| title_full_unstemmed | Aging-associated prostate smooth muscle hypercontractility in rats |
| title_short | Aging-associated prostate smooth muscle hypercontractility in rats |
| title_sort | aging associated prostate smooth muscle hypercontractility in rats |
| topic | Benign prostatic hyperplasia Lower urinary tract symptoms Phenylephrine Electrical-field stimulation Smooth muscle |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100860&tlng=en |
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