Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila
Aeromonas hydrophila is an emerging foodborne pathogen causing human gastroenteritis. Aeromonas species isolated from food such as seafood presented multidrug-resistance (MDR), raising serious concerns regarding food safety and public health. The use of phages to infect bacteria is a defense against...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1178876/full |
| _version_ | 1797798486577512448 |
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| author | Yuting Hou Zhihao Wu Li Ren Yuan Chen Yong-An Zhang Yong-An Zhang Yang Zhou Yang Zhou Yang Zhou |
| author_facet | Yuting Hou Zhihao Wu Li Ren Yuan Chen Yong-An Zhang Yong-An Zhang Yang Zhou Yang Zhou Yang Zhou |
| author_sort | Yuting Hou |
| collection | DOAJ |
| description | Aeromonas hydrophila is an emerging foodborne pathogen causing human gastroenteritis. Aeromonas species isolated from food such as seafood presented multidrug-resistance (MDR), raising serious concerns regarding food safety and public health. The use of phages to infect bacteria is a defense against drug-resistant pathogens. In this study, phage ZPAH34 isolated from the lake sample exerted lytic activity against MDR A. hydrophila strain ZYAH75 and inhibited the biofilm on different food-contacting surfaces. ZPAH34 has a large dsDNA genome of 234 kb which belongs to a novel jumbo phage. However, its particle size is the smallest of known jumbo phages so far. Based on phylogenetic analysis, ZPAH34 was used to establish a new genus Chaoshanvirus. Biological characterization revealed that ZPAH34 exhibited wide environmental tolerance, and a high rapid adsorb and reproductive capacity. Food biocontrol experiments demonstrated that ZPAH34 reduces the viable count of A. hydrophila on fish fillets (2.31 log) and lettuce (3.28 log) with potential bactericidal effects. This study isolated and characterized jumbo phage ZPAH34 not only enriched the understanding of phage biological entity diversity and evolution because of its minimal virion size with large genome but also was the first usage of jumbo phage in food safety to eliminate A. hydrophila. |
| first_indexed | 2024-03-13T04:04:27Z |
| format | Article |
| id | doaj.art-9cdf759750574547ad6ada14bc84a335 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T04:04:27Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9cdf759750574547ad6ada14bc84a3352023-06-21T09:36:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11788761178876Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophilaYuting Hou0Zhihao Wu1Li Ren2Yuan Chen3Yong-An Zhang4Yong-An Zhang5Yang Zhou6Yang Zhou7Yang Zhou8National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Shenzhen Institute of Nutrition and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Shenzhen Institute of Nutrition and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Shenzhen Institute of Nutrition and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Shenzhen Institute of Nutrition and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Shenzhen Institute of Nutrition and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaNational Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Shenzhen Institute of Nutrition and Health, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaShenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, ChinaAeromonas hydrophila is an emerging foodborne pathogen causing human gastroenteritis. Aeromonas species isolated from food such as seafood presented multidrug-resistance (MDR), raising serious concerns regarding food safety and public health. The use of phages to infect bacteria is a defense against drug-resistant pathogens. In this study, phage ZPAH34 isolated from the lake sample exerted lytic activity against MDR A. hydrophila strain ZYAH75 and inhibited the biofilm on different food-contacting surfaces. ZPAH34 has a large dsDNA genome of 234 kb which belongs to a novel jumbo phage. However, its particle size is the smallest of known jumbo phages so far. Based on phylogenetic analysis, ZPAH34 was used to establish a new genus Chaoshanvirus. Biological characterization revealed that ZPAH34 exhibited wide environmental tolerance, and a high rapid adsorb and reproductive capacity. Food biocontrol experiments demonstrated that ZPAH34 reduces the viable count of A. hydrophila on fish fillets (2.31 log) and lettuce (3.28 log) with potential bactericidal effects. This study isolated and characterized jumbo phage ZPAH34 not only enriched the understanding of phage biological entity diversity and evolution because of its minimal virion size with large genome but also was the first usage of jumbo phage in food safety to eliminate A. hydrophila.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1178876/fullAeromonas hydrophilaphage ZPAH34genomefoodsbiofilm |
| spellingShingle | Yuting Hou Zhihao Wu Li Ren Yuan Chen Yong-An Zhang Yong-An Zhang Yang Zhou Yang Zhou Yang Zhou Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila Frontiers in Microbiology Aeromonas hydrophila phage ZPAH34 genome foods biofilm |
| title | Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila |
| title_full | Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila |
| title_fullStr | Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila |
| title_full_unstemmed | Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila |
| title_short | Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila |
| title_sort | characterization and application of a lytic jumbo phage zpah34 against multidrug resistant aeromonas hydrophila |
| topic | Aeromonas hydrophila phage ZPAH34 genome foods biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1178876/full |
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