IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer
Abstract Background IGF2BP3 expression is associated with poor prognosis in cancers of multiple tissue origins. However, the precise mechanism of its co-carcinogenic action in bladder cancer is unknown. Methods We aimed to demonstrate the relationship between IGF2BP3 expression and pan-cancer using...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-022-10353-5 |
| _version_ | 1811171711410765824 |
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| author | Wei Huang Lizhen Zhu Haoxuan Huang Yuanyuan Li Gongxian Wang Cheng Zhang |
| author_facet | Wei Huang Lizhen Zhu Haoxuan Huang Yuanyuan Li Gongxian Wang Cheng Zhang |
| author_sort | Wei Huang |
| collection | DOAJ |
| description | Abstract Background IGF2BP3 expression is associated with poor prognosis in cancers of multiple tissue origins. However, the precise mechanism of its co-carcinogenic action in bladder cancer is unknown. Methods We aimed to demonstrate the relationship between IGF2BP3 expression and pan-cancer using The Cancer Genome Atlas (TCGA) database. We next validated IGF2BP3 expression in the Gene Expression Omnibus (GEO) database (GSE3167). Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic values of IGF2BP3. Cox and logistic regression were used to explore the factors affecting the prognosis. Protein–protein interactions (PPIs) network was constructed by STRING. Enrichment analyses were performed to infer involved pathways and functional categories of IGF2BP3 using the cluster Profiler package. We applied single-sample gene set enrichment analysis (ssGSEA) algorithm and TIMER database to evaluate the expression level of immune genes. Results Pan-cancer analyses reveal that IGF2BP3 was higher in most cancer types, including bladder cancer, and the same results were found in GSE3167. The area under the ROC curve of IGF2BP3 was 0.736, which indicated that IGF2BP3 may be a potential diagnostic biomarker. High IGF2BP3 expression was associated with poorer overall survival (OS) (P = 0.015). For validation, we collected 95 bladder cancer samples and found that IGF2BP3 expression was higher in bladder cancer tissues than that in non-tumor bladder tissues by immunohistochemistry staining. We found a positive correlation between the expression level of IGF2BP3 and the clinical stage of bladder cancer. Immunocyte infiltration analysis showed that high IGF2BP3 expression was correlated with regulating the infiltration level of immune cell, including neutrophil cells and macrophages. IGF2BP3 promotes migration and invasion of bladder cancer cells, while IGF2BP3 inhibition had the opposite effects. Higher IGF2BP3 expression was closely associated with advanced TNM stage. Conclusion IGF2BP3 overexpression was related to disease progression and poor prognosis, as well as infiltration of immune cells in bladder cancer. IGF2BP3 can be a promising independent prognostic biomarker and potential treatment target for bladder cancer. |
| first_indexed | 2024-04-10T17:18:34Z |
| format | Article |
| id | doaj.art-9ce004819a884329a7d185f99082d5d4 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-10T17:18:34Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9ce004819a884329a7d185f99082d5d42023-02-05T12:15:41ZengBMCBMC Cancer1471-24072023-02-0123111810.1186/s12885-022-10353-5IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancerWei Huang0Lizhen Zhu1Haoxuan Huang2Yuanyuan Li3Gongxian Wang4Cheng Zhang5Department of Urology, The First Affiliated Hospital of Nanchang UniversityDepartment of Oncology, The First Affiliated Hospital of Nanchang UniversityDepartment of Urology, The First Affiliated Hospital of Nanchang UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang UniversityDepartment of Urology, The First Affiliated Hospital of Nanchang UniversityDepartment of Urology, The First Affiliated Hospital of Nanchang UniversityAbstract Background IGF2BP3 expression is associated with poor prognosis in cancers of multiple tissue origins. However, the precise mechanism of its co-carcinogenic action in bladder cancer is unknown. Methods We aimed to demonstrate the relationship between IGF2BP3 expression and pan-cancer using The Cancer Genome Atlas (TCGA) database. We next validated IGF2BP3 expression in the Gene Expression Omnibus (GEO) database (GSE3167). Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic values of IGF2BP3. Cox and logistic regression were used to explore the factors affecting the prognosis. Protein–protein interactions (PPIs) network was constructed by STRING. Enrichment analyses were performed to infer involved pathways and functional categories of IGF2BP3 using the cluster Profiler package. We applied single-sample gene set enrichment analysis (ssGSEA) algorithm and TIMER database to evaluate the expression level of immune genes. Results Pan-cancer analyses reveal that IGF2BP3 was higher in most cancer types, including bladder cancer, and the same results were found in GSE3167. The area under the ROC curve of IGF2BP3 was 0.736, which indicated that IGF2BP3 may be a potential diagnostic biomarker. High IGF2BP3 expression was associated with poorer overall survival (OS) (P = 0.015). For validation, we collected 95 bladder cancer samples and found that IGF2BP3 expression was higher in bladder cancer tissues than that in non-tumor bladder tissues by immunohistochemistry staining. We found a positive correlation between the expression level of IGF2BP3 and the clinical stage of bladder cancer. Immunocyte infiltration analysis showed that high IGF2BP3 expression was correlated with regulating the infiltration level of immune cell, including neutrophil cells and macrophages. IGF2BP3 promotes migration and invasion of bladder cancer cells, while IGF2BP3 inhibition had the opposite effects. Higher IGF2BP3 expression was closely associated with advanced TNM stage. Conclusion IGF2BP3 overexpression was related to disease progression and poor prognosis, as well as infiltration of immune cells in bladder cancer. IGF2BP3 can be a promising independent prognostic biomarker and potential treatment target for bladder cancer.https://doi.org/10.1186/s12885-022-10353-5IGF2BP3Bladder cancerPDL-1Prognosis marker |
| spellingShingle | Wei Huang Lizhen Zhu Haoxuan Huang Yuanyuan Li Gongxian Wang Cheng Zhang IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer BMC Cancer IGF2BP3 Bladder cancer PDL-1 Prognosis marker |
| title | IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer |
| title_full | IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer |
| title_fullStr | IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer |
| title_full_unstemmed | IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer |
| title_short | IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer |
| title_sort | igf2bp3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer |
| topic | IGF2BP3 Bladder cancer PDL-1 Prognosis marker |
| url | https://doi.org/10.1186/s12885-022-10353-5 |
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