Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct

Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct

Tobacco etch virus Protease (TEVp), a cysteine protease, is renowned for its remarkable specific proteolysis, making it an invaluable tool for removing fusion tags from recombinant proteins. However, TEV protease's inherent insolubility limits its broad application. Fusion constructs like an N-...

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Bibliographic Details
Main Authors: Pragyan P. Parida, Deepa Saraswathi, Subbarao M.V. Mopidevi, Sreejith Raran-Kurussi
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Current Research in Structural Biology
Subjects:
NT*
Fusion protein
TEV protease
Cleavage site
Purification
Enzymatic activity
Online Access:http://www.sciencedirect.com/science/article/pii/S2665928X23000120

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