Opticool: Cutting-edge transgenic optical tools.
Only a few short decades have passed since the sequencing of GFP, yet the modern repertoire of transgenically encoded optical tools implies an exponential proliferation of ever improving constructions to interrogate the subcellular environment. A myriad of tags for labeling proteins, RNA, or DNA hav...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-03-01
|
| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011208&type=printable |
| _version_ | 1797238480697294848 |
|---|---|
| author | Kelli D Fenelon Julia Krause Theodora Koromila |
| author_facet | Kelli D Fenelon Julia Krause Theodora Koromila |
| author_sort | Kelli D Fenelon |
| collection | DOAJ |
| description | Only a few short decades have passed since the sequencing of GFP, yet the modern repertoire of transgenically encoded optical tools implies an exponential proliferation of ever improving constructions to interrogate the subcellular environment. A myriad of tags for labeling proteins, RNA, or DNA have arisen in the last few decades, facilitating unprecedented visualization of subcellular components and processes. Development of a broad array of modern genetically encoded sensors allows real-time, in vivo detection of molecule levels, pH, forces, enzyme activity, and other subcellular and extracellular phenomena in ever expanding contexts. Optogenetic, genetically encoded optically controlled manipulation systems have gained traction in the biological research community and facilitate single-cell, real-time modulation of protein function in vivo in ever broadening, novel applications. While this field continues to explosively expand, references are needed to assist scientists seeking to use and improve these transgenic devices in new and exciting ways to interrogate development and disease. In this review, we endeavor to highlight the state and trajectory of the field of in vivo transgenic optical tools. |
| first_indexed | 2024-04-24T17:36:19Z |
| format | Article |
| id | doaj.art-9ce0e2510d5f40939d339ff8122869cf |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-24T17:36:19Z |
| publishDate | 2024-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-9ce0e2510d5f40939d339ff8122869cf2024-03-28T05:34:19ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042024-03-01203e101120810.1371/journal.pgen.1011208Opticool: Cutting-edge transgenic optical tools.Kelli D FenelonJulia KrauseTheodora KoromilaOnly a few short decades have passed since the sequencing of GFP, yet the modern repertoire of transgenically encoded optical tools implies an exponential proliferation of ever improving constructions to interrogate the subcellular environment. A myriad of tags for labeling proteins, RNA, or DNA have arisen in the last few decades, facilitating unprecedented visualization of subcellular components and processes. Development of a broad array of modern genetically encoded sensors allows real-time, in vivo detection of molecule levels, pH, forces, enzyme activity, and other subcellular and extracellular phenomena in ever expanding contexts. Optogenetic, genetically encoded optically controlled manipulation systems have gained traction in the biological research community and facilitate single-cell, real-time modulation of protein function in vivo in ever broadening, novel applications. While this field continues to explosively expand, references are needed to assist scientists seeking to use and improve these transgenic devices in new and exciting ways to interrogate development and disease. In this review, we endeavor to highlight the state and trajectory of the field of in vivo transgenic optical tools.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011208&type=printable |
| spellingShingle | Kelli D Fenelon Julia Krause Theodora Koromila Opticool: Cutting-edge transgenic optical tools. PLoS Genetics |
| title | Opticool: Cutting-edge transgenic optical tools. |
| title_full | Opticool: Cutting-edge transgenic optical tools. |
| title_fullStr | Opticool: Cutting-edge transgenic optical tools. |
| title_full_unstemmed | Opticool: Cutting-edge transgenic optical tools. |
| title_short | Opticool: Cutting-edge transgenic optical tools. |
| title_sort | opticool cutting edge transgenic optical tools |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011208&type=printable |
| work_keys_str_mv | AT kellidfenelon opticoolcuttingedgetransgenicopticaltools AT juliakrause opticoolcuttingedgetransgenicopticaltools AT theodorakoromila opticoolcuttingedgetransgenicopticaltools |