Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets
Dietary fiber (DF) is increasingly thought to regulate diversity of piglet gut microbiota to alleviate weaning stress in piglets. This study was conducted to investigate the effects of DF on growth performance of piglets and composition of their gut microbiota, as well as the interaction between gut...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02344/full |
| _version_ | 1819151754789388288 |
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| author | Boshuai Liu Wenjing Wang Xiaoyan Zhu Xiaoyan Zhu Xiao Sun Junnan Xiao Defeng Li Defeng Li Yalei Cui Yalei Cui Chengzhang Wang Chengzhang Wang Yinghua Shi Yinghua Shi |
| author_facet | Boshuai Liu Wenjing Wang Xiaoyan Zhu Xiaoyan Zhu Xiao Sun Junnan Xiao Defeng Li Defeng Li Yalei Cui Yalei Cui Chengzhang Wang Chengzhang Wang Yinghua Shi Yinghua Shi |
| author_sort | Boshuai Liu |
| collection | DOAJ |
| description | Dietary fiber (DF) is increasingly thought to regulate diversity of piglet gut microbiota to alleviate weaning stress in piglets. This study was conducted to investigate the effects of DF on growth performance of piglets and composition of their gut microbiota, as well as the interaction between gut microbiota and short-chain fatty acids (SCFAs) in piglets. A total of 840 piglets were allocated to three dietary treatments consisting of a control group (CG), an alfalfa meal group (AG), and a commodity concentrated fiber group (OG) in a 30-day feeding trial. Gut mucosa and feces samples were used to determine bacterial community diversity by 16S rRNA gene amplicon sequencing. Fiber treatment had a positive effect on growth performance and metabolism of SCFAs in piglets, in particular, compared with CG, the diarrhea rate was significantly decreased, and the content of propionic acid (PA) in the cecum was markedly increased in AG. The Shannon indices of the jejunum microbiota in AG were higher than CG. At the genus level, compared to CG, in the duodenum, the relative abundance of Paenibacillus in AG and OG was higher; in the jejunum, the relative abundances of Bacillus, Oceanobacillus, Paenibacillus, Lactococcus, Enterococcus, and Exiguobacterium were higher, whereas the relative abundance of Mycoplasma was lower in AG; in the cecum, there was also lower relative abundance of Helicobacter in AG and OG, and furthermore, the relative abundance of Faecalibacterium in OG was higher than in CG and AG. Spearman correlation analysis showed that Pseudobutyrivibrio was positively correlated with acetic acid, PA, and butyric acid (BA), while Bacteroides and Anaerotruncus were negatively correlated with PA and BA. In addition, microbiota analyses among different intestine segments showed distinct differences in microbiota between the proximal and distal intestines. Bacteria in the proximal segments were mainly Firmicutes, while bacteria in the distal segments were mainly Bacteroidetes and Firmicutes. Overall, these findings suggested that DF treatment could reduce the diarrhea rate of piglets and had beneficial effects on gut health, which might be attributed to the alteration in gut microbiota induced by DF and the interaction of the gut microbiota with SCFAs. |
| first_indexed | 2024-12-22T14:38:26Z |
| format | Article |
| id | doaj.art-9ce397dfec844fbf8b30ce1885b1d763 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T14:38:26Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9ce397dfec844fbf8b30ce1885b1d7632022-12-21T18:22:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02344397703Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in PigletsBoshuai Liu0Wenjing Wang1Xiaoyan Zhu2Xiaoyan Zhu3Xiao Sun4Junnan Xiao5Defeng Li6Defeng Li7Yalei Cui8Yalei Cui9Chengzhang Wang10Chengzhang Wang11Yinghua Shi12Yinghua Shi13College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, ChinaDietary fiber (DF) is increasingly thought to regulate diversity of piglet gut microbiota to alleviate weaning stress in piglets. This study was conducted to investigate the effects of DF on growth performance of piglets and composition of their gut microbiota, as well as the interaction between gut microbiota and short-chain fatty acids (SCFAs) in piglets. A total of 840 piglets were allocated to three dietary treatments consisting of a control group (CG), an alfalfa meal group (AG), and a commodity concentrated fiber group (OG) in a 30-day feeding trial. Gut mucosa and feces samples were used to determine bacterial community diversity by 16S rRNA gene amplicon sequencing. Fiber treatment had a positive effect on growth performance and metabolism of SCFAs in piglets, in particular, compared with CG, the diarrhea rate was significantly decreased, and the content of propionic acid (PA) in the cecum was markedly increased in AG. The Shannon indices of the jejunum microbiota in AG were higher than CG. At the genus level, compared to CG, in the duodenum, the relative abundance of Paenibacillus in AG and OG was higher; in the jejunum, the relative abundances of Bacillus, Oceanobacillus, Paenibacillus, Lactococcus, Enterococcus, and Exiguobacterium were higher, whereas the relative abundance of Mycoplasma was lower in AG; in the cecum, there was also lower relative abundance of Helicobacter in AG and OG, and furthermore, the relative abundance of Faecalibacterium in OG was higher than in CG and AG. Spearman correlation analysis showed that Pseudobutyrivibrio was positively correlated with acetic acid, PA, and butyric acid (BA), while Bacteroides and Anaerotruncus were negatively correlated with PA and BA. In addition, microbiota analyses among different intestine segments showed distinct differences in microbiota between the proximal and distal intestines. Bacteria in the proximal segments were mainly Firmicutes, while bacteria in the distal segments were mainly Bacteroidetes and Firmicutes. Overall, these findings suggested that DF treatment could reduce the diarrhea rate of piglets and had beneficial effects on gut health, which might be attributed to the alteration in gut microbiota induced by DF and the interaction of the gut microbiota with SCFAs.https://www.frontiersin.org/article/10.3389/fmicb.2018.02344/fulldietary fibershort-chain fatty acidgut microbiotadiarrhea ratepiglets |
| spellingShingle | Boshuai Liu Wenjing Wang Xiaoyan Zhu Xiaoyan Zhu Xiao Sun Junnan Xiao Defeng Li Defeng Li Yalei Cui Yalei Cui Chengzhang Wang Chengzhang Wang Yinghua Shi Yinghua Shi Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets Frontiers in Microbiology dietary fiber short-chain fatty acid gut microbiota diarrhea rate piglets |
| title | Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets |
| title_full | Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets |
| title_fullStr | Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets |
| title_full_unstemmed | Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets |
| title_short | Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets |
| title_sort | response of gut microbiota to dietary fiber and metabolic interaction with scfas in piglets |
| topic | dietary fiber short-chain fatty acid gut microbiota diarrhea rate piglets |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02344/full |
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