MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3
Objectives: Ovarian cancer is the most lethal gynecologic malignancy, and targeted therapy for different pathological types and molecular phenotypes is urgent to be studied. Studies have shown that MicroRNA-592 (miR-592) plays an important negative regulatory role in the occurrence of gastrointestin...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-03-01
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| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338231157156 |
| _version_ | 1797871018732158976 |
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| author | Qiong Jin MD Ning Zhang MD Yang Zhan MD Xiaohong Xu MD Chao Han MD Hui Zhao MD Xiaodi Hu MD Hongbo Tang MD Yumei Wu MD |
| author_facet | Qiong Jin MD Ning Zhang MD Yang Zhan MD Xiaohong Xu MD Chao Han MD Hui Zhao MD Xiaodi Hu MD Hongbo Tang MD Yumei Wu MD |
| author_sort | Qiong Jin MD |
| collection | DOAJ |
| description | Objectives: Ovarian cancer is the most lethal gynecologic malignancy, and targeted therapy for different pathological types and molecular phenotypes is urgent to be studied. Studies have shown that MicroRNA-592 (miR-592) plays an important negative regulatory role in the occurrence of gastrointestinal malignancies, breast cancer, non-small cell lung cancer, and glioma, but the expression of miR-592 in ovarian cancer and the mechanism of action are still unclear. Methods: The expressions of miR-592 were examined by RT-PCR and Western Blot. Cell viability and migratory capacity were detected by CCK-8 and transwell assay. TargetScan ( http://www.targetscan.org ) was analyzed to predict potential targets of miR-592. Then Dual-luciferase reporter gene assay was performed to verify the targeting relationship between miR-592 and ERBB3. A mouse xenograft model was applied to confirm the effect of miR-592. Results: In our study, we found that the expression of miR-592 is reduced in epithelial ovarian cancer tissues. The exogenous expression of miR-592 inhibits the proliferation, migration, and invasion in epithelial ovarian cancer tumor cells. Furthermore, the exogenous expression of miR-592 inhibits tumor growth in the nude mouse xenograft model. Therefore, miR-592 may play a role of tumor suppressor miRNA in the occurrence and development of ovarian cancer. Further experiments demonstrated that tumor-related ERBB3 is a target gene mediated by miRNA-592. The dual-luciferase reporter system was used to identify miRNA-592 target genes; qPCR and Western Blot were used to detect the expression of ERBB3. Mechanical experiments confirmed that miRNA-592 negatively regulated ERBB3. Conclusion: Together, these findings identify a heretofore unrecognized link between miR-592 and ERBB3 and suggest that targeting on miR-592 warrants attention as a novel and potential therapeutic strategy for ovarian cancer. |
| first_indexed | 2024-04-10T00:37:39Z |
| format | Article |
| id | doaj.art-9ce563c705e94834a912c862d670093f |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-04-10T00:37:39Z |
| publishDate | 2023-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-9ce563c705e94834a912c862d670093f2023-03-14T13:05:00ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382023-03-012210.1177/15330338231157156MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3Qiong Jin MD0Ning Zhang MD1Yang Zhan MD2Xiaohong Xu MD3Chao Han MD4Hui Zhao MD5 Xiaodi Hu MD6Hongbo Tang MD7Yumei Wu MD8 , Beijing Maternal and Child Health Care Hospital, Beijing, China Department of Intensive Care Medicine, Yantai Penglai Traditional Chinese Medicine Hospital, Yantai, China Department of Pathology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China , Beijing Maternal and Child Health Care Hospital, Beijing, China , Beijing Maternal and Child Health Care Hospital, Beijing, China , Beijing Maternal and Child Health Care Hospital, Beijing, China , Beijing Maternal and Child Health Care Hospital, Beijing, China Department of Pharmacy, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China , Beijing Maternal and Child Health Care Hospital, Beijing, ChinaObjectives: Ovarian cancer is the most lethal gynecologic malignancy, and targeted therapy for different pathological types and molecular phenotypes is urgent to be studied. Studies have shown that MicroRNA-592 (miR-592) plays an important negative regulatory role in the occurrence of gastrointestinal malignancies, breast cancer, non-small cell lung cancer, and glioma, but the expression of miR-592 in ovarian cancer and the mechanism of action are still unclear. Methods: The expressions of miR-592 were examined by RT-PCR and Western Blot. Cell viability and migratory capacity were detected by CCK-8 and transwell assay. TargetScan ( http://www.targetscan.org ) was analyzed to predict potential targets of miR-592. Then Dual-luciferase reporter gene assay was performed to verify the targeting relationship between miR-592 and ERBB3. A mouse xenograft model was applied to confirm the effect of miR-592. Results: In our study, we found that the expression of miR-592 is reduced in epithelial ovarian cancer tissues. The exogenous expression of miR-592 inhibits the proliferation, migration, and invasion in epithelial ovarian cancer tumor cells. Furthermore, the exogenous expression of miR-592 inhibits tumor growth in the nude mouse xenograft model. Therefore, miR-592 may play a role of tumor suppressor miRNA in the occurrence and development of ovarian cancer. Further experiments demonstrated that tumor-related ERBB3 is a target gene mediated by miRNA-592. The dual-luciferase reporter system was used to identify miRNA-592 target genes; qPCR and Western Blot were used to detect the expression of ERBB3. Mechanical experiments confirmed that miRNA-592 negatively regulated ERBB3. Conclusion: Together, these findings identify a heretofore unrecognized link between miR-592 and ERBB3 and suggest that targeting on miR-592 warrants attention as a novel and potential therapeutic strategy for ovarian cancer.https://doi.org/10.1177/15330338231157156 |
| spellingShingle | Qiong Jin MD Ning Zhang MD Yang Zhan MD Xiaohong Xu MD Chao Han MD Hui Zhao MD Xiaodi Hu MD Hongbo Tang MD Yumei Wu MD MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 Technology in Cancer Research & Treatment |
| title | MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 |
| title_full | MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 |
| title_fullStr | MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 |
| title_full_unstemmed | MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 |
| title_short | MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 |
| title_sort | microrna 592 inhibits the growth of ovarian cancer cells by targeting erbb3 |
| url | https://doi.org/10.1177/15330338231157156 |
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