RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling

RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling

Abstract Background Escherichia coli is a cost-effective expression system for production of antibody fragments like Fabs. Various yield improvement strategies have been applied, however, Fabs remain challenging to produce. This study aimed to characterize the gene expression response of commonly us...

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Bibliographic Details
Main Authors: Sophie Vazulka, Matteo Schiavinato, Christopher Tauer, Martin Wagenknecht, Monika Cserjan-Puschmann, Gerald Striedner
Format: Article
Language:English
Published: BMC 2024-01-01
Series:Microbial Cell Factories
Subjects:
Recombinant protein production
Periplasmic expression
Transcriptomics
Envelope stress
Ribosome stalling
Polyproline
Online Access:https://doi.org/10.1186/s12934-023-02278-w

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