Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum
On PDA-benomyl plates growth of Trichoderma harzianum was inhibited by 20 and 30 % at benomyl 1 and 2 μg/mL, respectively, and was completely inhibited at 5 μg/mL. In minimal synthetic medium (MSM) amended with different concentrations of benomyl (1.0, 3.0, 5.0, 7.0 and 10.0 μg/mL), fungal immobilis...
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| Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2004-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/163178 |
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| author | Mohammed S. El-Katatny Gehan M. Shaban Ahmed M. A. Hetta Momein H. El-Katatny Hesham M. El-Komy |
| author_facet | Mohammed S. El-Katatny Gehan M. Shaban Ahmed M. A. Hetta Momein H. El-Katatny Hesham M. El-Komy |
| author_sort | Mohammed S. El-Katatny |
| collection | DOAJ |
| description | On PDA-benomyl plates growth of Trichoderma harzianum was inhibited by 20 and 30 % at benomyl 1 and 2 μg/mL, respectively, and was completely inhibited at 5 μg/mL. In minimal synthetic medium (MSM) amended with different concentrations of benomyl (1.0, 3.0, 5.0, 7.0 and 10.0 μg/mL), fungal immobilisation improved chitinase and β-1,3-glucanase production at low benomyl concentrations (1, 3 and 5 μg/mL). Further increase in the production of both enzymes was obtained by immobilisation at higher benomyl concentrations (7 and 10 μg/mL). Fungal immobilisation increased bound chitinase by 15- to 30-fold at 3 and 5 μg/mL benomyl concentration, respectively. However, no effect was obtained on the bound β-1,3-glucanase. Different benomyl concentrations (0.3 to 1500 μg/mL) had no significant inhibitory effect on the activities of free or immobilised chitinase and β-1,3-glucanase. It could be suggested that either immobilised Trichoderma or immobilised chitinase and β-1,3-glucanase could be used in combination with benomyl to control plant pathogens. |
| first_indexed | 2024-03-09T08:25:46Z |
| format | Article |
| id | doaj.art-9ce9f8ba1c7a49c9a385ae60715d0de8 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:25:46Z |
| publishDate | 2004-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-9ce9f8ba1c7a49c9a385ae60715d0de82023-12-02T21:10:14ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062004-01-014228388Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianumMohammed S. El-Katatny0Gehan M. Shaban1Ahmed M. A. Hetta2Momein H. El-Katatny3Hesham M. El-Komy4Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, EgyptDepartment of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, EgyptDepartment of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, EgyptDepartment of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, EgyptDepartment of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, EgyptOn PDA-benomyl plates growth of Trichoderma harzianum was inhibited by 20 and 30 % at benomyl 1 and 2 μg/mL, respectively, and was completely inhibited at 5 μg/mL. In minimal synthetic medium (MSM) amended with different concentrations of benomyl (1.0, 3.0, 5.0, 7.0 and 10.0 μg/mL), fungal immobilisation improved chitinase and β-1,3-glucanase production at low benomyl concentrations (1, 3 and 5 μg/mL). Further increase in the production of both enzymes was obtained by immobilisation at higher benomyl concentrations (7 and 10 μg/mL). Fungal immobilisation increased bound chitinase by 15- to 30-fold at 3 and 5 μg/mL benomyl concentration, respectively. However, no effect was obtained on the bound β-1,3-glucanase. Different benomyl concentrations (0.3 to 1500 μg/mL) had no significant inhibitory effect on the activities of free or immobilised chitinase and β-1,3-glucanase. It could be suggested that either immobilised Trichoderma or immobilised chitinase and β-1,3-glucanase could be used in combination with benomyl to control plant pathogens.http://hrcak.srce.hr/file/163178Trichoderma spp.chitinaseglucanasebenomylfungicidesCa-alginate |
| spellingShingle | Mohammed S. El-Katatny Gehan M. Shaban Ahmed M. A. Hetta Momein H. El-Katatny Hesham M. El-Komy Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum Food Technology and Biotechnology Trichoderma spp. chitinase glucanase benomyl fungicides Ca-alginate |
| title | Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum |
| title_full | Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum |
| title_fullStr | Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum |
| title_full_unstemmed | Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum |
| title_short | Effect of Benomyl on Chitinase and β-1,3-Glucanase Production by Free and Alginate Encapsulated Trichoderma harzianum |
| title_sort | effect of benomyl on chitinase and β 1 3 glucanase production by free and alginate encapsulated trichoderma harzianum |
| topic | Trichoderma spp. chitinase glucanase benomyl fungicides Ca-alginate |
| url | http://hrcak.srce.hr/file/163178 |
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