Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles
Indoor air pollutants (IAP), which can pose a serious risk to human health, include biological pollutants, nitric oxide (NO), nitrogen dioxide (NO<sub>2</sub>), volatile organic compounds (VOC), sulfur dioxide (SO<sub>2</sub>), carbon monoxide (CO), carbon dioxide (CO<sub&...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/2/226 |
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| author | Sabrina Colafarina Piero Di Carlo Osvaldo Zarivi Massimo Aloisi Alessandra Di Serafino Eleonora Aruffo Lorenzo Arrizza Tania Limongi Anna Poma |
| author_facet | Sabrina Colafarina Piero Di Carlo Osvaldo Zarivi Massimo Aloisi Alessandra Di Serafino Eleonora Aruffo Lorenzo Arrizza Tania Limongi Anna Poma |
| author_sort | Sabrina Colafarina |
| collection | DOAJ |
| description | Indoor air pollutants (IAP), which can pose a serious risk to human health, include biological pollutants, nitric oxide (NO), nitrogen dioxide (NO<sub>2</sub>), volatile organic compounds (VOC), sulfur dioxide (SO<sub>2</sub>), carbon monoxide (CO), carbon dioxide (CO<sub>2</sub>), silica, metals, radon, and particulate matter (PM). The aim of our work is to conduct a multidisciplinary study of fine silica particles (<2.5 μm) in the presence or absence of ozone (O<sub>3</sub>), and evaluate their potential cytotoxicity using MTS, micronucleus, and the comet test in two cell lines. We analyzed A549 (human basal alveolar epithelial cell adenocarcinoma) and Hs27 (human normal fibroblasts) exposed to dynamic conditions by an IRC simulator under ozone flow (120 ppb) and in the presence of silica particles (40 μg/h). The viability of A549 and Hs27 cells at 48 and 72 h of exposure to silica or silica/ozone decreases, except at 72 h in Hs27 treated with silica/ozone. The micronucleus and comet tests showed a significant increase in the number of micronuclei and the % of DNA in the queue, compared to the control, in both lines in all treatments, even if in different cell times/types. We found that silica alone or with more O<sub>3</sub> causes more pronounced genotoxic effects in A549 tumor cells than in normal Hs27 fibroblasts. |
| first_indexed | 2024-03-10T01:44:25Z |
| format | Article |
| id | doaj.art-9cea790a34b84c069fc86cc8609385c0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:44:25Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-9cea790a34b84c069fc86cc8609385c02023-11-23T13:18:05ZengMDPI AGCells2073-44092022-01-0111222610.3390/cells11020226Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica MicroparticlesSabrina Colafarina0Piero Di Carlo1Osvaldo Zarivi2Massimo Aloisi3Alessandra Di Serafino4Eleonora Aruffo5Lorenzo Arrizza6Tania Limongi7Anna Poma8Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, ItalyDepartment of Psychological, Health and Territorial Sciences, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, ItalyDepartment of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, ItalyDepartment of Psychological, Health and Territorial Sciences, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Psychological, Health and Territorial Sciences, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, ItalyDepartment of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, ItalyDepartment of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, ItalyIndoor air pollutants (IAP), which can pose a serious risk to human health, include biological pollutants, nitric oxide (NO), nitrogen dioxide (NO<sub>2</sub>), volatile organic compounds (VOC), sulfur dioxide (SO<sub>2</sub>), carbon monoxide (CO), carbon dioxide (CO<sub>2</sub>), silica, metals, radon, and particulate matter (PM). The aim of our work is to conduct a multidisciplinary study of fine silica particles (<2.5 μm) in the presence or absence of ozone (O<sub>3</sub>), and evaluate their potential cytotoxicity using MTS, micronucleus, and the comet test in two cell lines. We analyzed A549 (human basal alveolar epithelial cell adenocarcinoma) and Hs27 (human normal fibroblasts) exposed to dynamic conditions by an IRC simulator under ozone flow (120 ppb) and in the presence of silica particles (40 μg/h). The viability of A549 and Hs27 cells at 48 and 72 h of exposure to silica or silica/ozone decreases, except at 72 h in Hs27 treated with silica/ozone. The micronucleus and comet tests showed a significant increase in the number of micronuclei and the % of DNA in the queue, compared to the control, in both lines in all treatments, even if in different cell times/types. We found that silica alone or with more O<sub>3</sub> causes more pronounced genotoxic effects in A549 tumor cells than in normal Hs27 fibroblasts.https://www.mdpi.com/2073-4409/11/2/226indoor air pollution (IAP)ozonesilica fine particlesPM 2.5genotoxicityA549 |
| spellingShingle | Sabrina Colafarina Piero Di Carlo Osvaldo Zarivi Massimo Aloisi Alessandra Di Serafino Eleonora Aruffo Lorenzo Arrizza Tania Limongi Anna Poma Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles Cells indoor air pollution (IAP) ozone silica fine particles PM 2.5 genotoxicity A549 |
| title | Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles |
| title_full | Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles |
| title_fullStr | Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles |
| title_full_unstemmed | Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles |
| title_short | Genotoxicity Response of Fibroblast Cells and Human Epithelial Adenocarcinoma In Vitro Model Exposed to Bare and Ozone-Treated Silica Microparticles |
| title_sort | genotoxicity response of fibroblast cells and human epithelial adenocarcinoma in vitro model exposed to bare and ozone treated silica microparticles |
| topic | indoor air pollution (IAP) ozone silica fine particles PM 2.5 genotoxicity A549 |
| url | https://www.mdpi.com/2073-4409/11/2/226 |
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