Chromosome walking with BAC clones as a method of genome mapping
Current sequencing projects are often based on random sequencing of genomic libraries followed by contig assembly by means of bioinformatics tools. This approach is convenient for whole genome sequencing projects. Chromosome walking described here is suitable for mapping and sequencing of short geno...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2007-10-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-200710-0005_chromosome-walking-with-bac-clones-as-a-method-of-genome-mapping.php |
| _version_ | 1797898363778105344 |
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| author | Z. Kubát |
| author_facet | Z. Kubát |
| author_sort | Z. Kubát |
| collection | DOAJ |
| description | Current sequencing projects are often based on random sequencing of genomic libraries followed by contig assembly by means of bioinformatics tools. This approach is convenient for whole genome sequencing projects. Chromosome walking described here is suitable for mapping and sequencing of short genomic regions in species where whole genome sequencing is not possible or for cloning gene from its closest known marker. This method is based on searching for overlapping BAC clones specific for the genomic region of interest. |
| first_indexed | 2024-04-10T08:12:13Z |
| format | Article |
| id | doaj.art-9cef0dc9a9fd433a87609124c887e841 |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:12:13Z |
| publishDate | 2007-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-9cef0dc9a9fd433a87609124c887e8412023-02-23T03:45:19ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682007-10-01531044745010.17221/2198-PSEpse-200710-0005Chromosome walking with BAC clones as a method of genome mappingZ. Kubát0Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech RepublicCurrent sequencing projects are often based on random sequencing of genomic libraries followed by contig assembly by means of bioinformatics tools. This approach is convenient for whole genome sequencing projects. Chromosome walking described here is suitable for mapping and sequencing of short genomic regions in species where whole genome sequencing is not possible or for cloning gene from its closest known marker. This method is based on searching for overlapping BAC clones specific for the genomic region of interest.https://pse.agriculturejournals.cz/artkey/pse-200710-0005_chromosome-walking-with-bac-clones-as-a-method-of-genome-mapping.phpbac librarysequencingphysical mapping |
| spellingShingle | Z. Kubát Chromosome walking with BAC clones as a method of genome mapping Plant, Soil and Environment bac library sequencing physical mapping |
| title | Chromosome walking with BAC clones as a method of genome mapping |
| title_full | Chromosome walking with BAC clones as a method of genome mapping |
| title_fullStr | Chromosome walking with BAC clones as a method of genome mapping |
| title_full_unstemmed | Chromosome walking with BAC clones as a method of genome mapping |
| title_short | Chromosome walking with BAC clones as a method of genome mapping |
| title_sort | chromosome walking with bac clones as a method of genome mapping |
| topic | bac library sequencing physical mapping |
| url | https://pse.agriculturejournals.cz/artkey/pse-200710-0005_chromosome-walking-with-bac-clones-as-a-method-of-genome-mapping.php |
| work_keys_str_mv | AT zkubat chromosomewalkingwithbacclonesasamethodofgenomemapping |