Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring
Abstract Background Pregnancy exposure to titanium dioxide nanoparticles (TiO2NPs) is a vital consideration due to their inadvertent ingestion from environmental contamination. The potential health effects of TiO2NPs on the neurodevelopmental process should be seriously concerned in health risk asse...
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-021-00967-5 |
| _version_ | 1828137073275568128 |
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| author | Junhao Su Xinhao Duan Yu Qiu Lixiao Zhou Hongyang Zhang Min Gao Yijun Liu Zhen Zou Jingfu Qiu Chengzhi Chen |
| author_facet | Junhao Su Xinhao Duan Yu Qiu Lixiao Zhou Hongyang Zhang Min Gao Yijun Liu Zhen Zou Jingfu Qiu Chengzhi Chen |
| author_sort | Junhao Su |
| collection | DOAJ |
| description | Abstract Background Pregnancy exposure to titanium dioxide nanoparticles (TiO2NPs) is a vital consideration due to their inadvertent ingestion from environmental contamination. The potential health effects of TiO2NPs on the neurodevelopmental process should be seriously concerned in health risk assessment, especially for the pregnant women who are susceptible to the neurodevelopmental toxicity of nano-sized particles. However, the available evidence of neurodevelopmental toxicity of TiO2NPs remains very limited. Methods In the present study, the pregnant mice were intragastric administered with 150 mg/kg TiO2NPs from gestational day (GD) 8 to 21, the maternal behaviors and neurodevelopment-related indicators in offspring were all assessed at different time points after delivery. The gut microbial community in both dams and their offspring were detected by using 16S ribosomal RNA (rRNA) gene sequencing. The gut-brain axis related indicators were also determined in the offspring. Results The results clearly demonstrated that exposure to TiO2NPs did not affect the maternal behaviors of pregnant mice, or cause the deficits on the developmental milestones and perturbations in the early postnatal development of offspring. Intriguingly, our data revealed that pregnancy exposure of TiO2NPs did not affect locomotor function, learning and memory ability and anxiety-like behavior in offspring at postnatal day (PD) 21, but resulted in obvious impairments on these neurobehaviors at PD49. Similar phenomena were obtained in the composition of gut microbial community, intestinal and brain pathological damage in offspring in adulthood. Moreover, the intestinal dysbiosis induced by TiO2NPs might be highly associated with the delayed appearance of neurobehavioral impairments in offspring, possibly occurring through disruption of gut-brain axis. Conclusions This is the first report elucidated that pregnancy exposure to TiO2NPs caused delayed appearance of neurobehavioral impairments in offspring when they reached adulthood, although these perturbations did not happen at early life after delivery. These findings will provide valuable insights about neurodevelopmental toxicity of TiO2NPs, and call for comprehensive health risk assessment of TiO2NPs on the susceptible population, such as pregnant women. Graphical abstract |
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| format | Article |
| id | doaj.art-9cf2e8bd74e34130a411a72c5f3600c5 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T18:13:30Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-9cf2e8bd74e34130a411a72c5f3600c52022-12-22T04:10:01ZengBMCJournal of Nanobiotechnology1477-31552021-08-0119112110.1186/s12951-021-00967-5Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspringJunhao Su0Xinhao Duan1Yu Qiu2Lixiao Zhou3Hongyang Zhang4Min Gao5Yijun Liu6Zhen Zou7Jingfu Qiu8Chengzhi Chen9Department of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityDepartment of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityDepartment of Neurology, The Affiliated University-Town Hospital of Chongqing Medical UniversityDepartment of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityDepartment of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityDepartment of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityDepartment of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityMolecular Biology Laboratory of Respiratory Diseases, Institute of Life Sciences, Chongqing Medical UniversityDepartment of Health Laboratory Technology, School of Public Health and Management, Chongqing Medical UniversityDongsheng Lung-Brain Disease Joint Lab, Chongqing Medical UniversityAbstract Background Pregnancy exposure to titanium dioxide nanoparticles (TiO2NPs) is a vital consideration due to their inadvertent ingestion from environmental contamination. The potential health effects of TiO2NPs on the neurodevelopmental process should be seriously concerned in health risk assessment, especially for the pregnant women who are susceptible to the neurodevelopmental toxicity of nano-sized particles. However, the available evidence of neurodevelopmental toxicity of TiO2NPs remains very limited. Methods In the present study, the pregnant mice were intragastric administered with 150 mg/kg TiO2NPs from gestational day (GD) 8 to 21, the maternal behaviors and neurodevelopment-related indicators in offspring were all assessed at different time points after delivery. The gut microbial community in both dams and their offspring were detected by using 16S ribosomal RNA (rRNA) gene sequencing. The gut-brain axis related indicators were also determined in the offspring. Results The results clearly demonstrated that exposure to TiO2NPs did not affect the maternal behaviors of pregnant mice, or cause the deficits on the developmental milestones and perturbations in the early postnatal development of offspring. Intriguingly, our data revealed that pregnancy exposure of TiO2NPs did not affect locomotor function, learning and memory ability and anxiety-like behavior in offspring at postnatal day (PD) 21, but resulted in obvious impairments on these neurobehaviors at PD49. Similar phenomena were obtained in the composition of gut microbial community, intestinal and brain pathological damage in offspring in adulthood. Moreover, the intestinal dysbiosis induced by TiO2NPs might be highly associated with the delayed appearance of neurobehavioral impairments in offspring, possibly occurring through disruption of gut-brain axis. Conclusions This is the first report elucidated that pregnancy exposure to TiO2NPs caused delayed appearance of neurobehavioral impairments in offspring when they reached adulthood, although these perturbations did not happen at early life after delivery. These findings will provide valuable insights about neurodevelopmental toxicity of TiO2NPs, and call for comprehensive health risk assessment of TiO2NPs on the susceptible population, such as pregnant women. Graphical abstracthttps://doi.org/10.1186/s12951-021-00967-5Titanium dioxide nanoparticlesIntestinal dysbiosisNeurobehavioral impairmentsPregnancy exposure |
| spellingShingle | Junhao Su Xinhao Duan Yu Qiu Lixiao Zhou Hongyang Zhang Min Gao Yijun Liu Zhen Zou Jingfu Qiu Chengzhi Chen Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring Journal of Nanobiotechnology Titanium dioxide nanoparticles Intestinal dysbiosis Neurobehavioral impairments Pregnancy exposure |
| title | Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring |
| title_full | Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring |
| title_fullStr | Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring |
| title_full_unstemmed | Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring |
| title_short | Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring |
| title_sort | pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring |
| topic | Titanium dioxide nanoparticles Intestinal dysbiosis Neurobehavioral impairments Pregnancy exposure |
| url | https://doi.org/10.1186/s12951-021-00967-5 |
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