Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice
Our experiment determined the immunotropic activity of a natural, iridoid-anthocyanin extract from honeysuckle berry (<i>Lonicera caerulea</i> L.) (LC). The extract was administered to mice infected with <i>Trichinella spiralis</i>, orally at a dose of 2 g/kg bw, six times at...
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MDPI AG
2022-03-01
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| author | Jolanta Piekarska Marianna Szczypka Michał Gorczykowski Anna Sokół-Łętowska Alicja Z. Kucharska |
| author_facet | Jolanta Piekarska Marianna Szczypka Michał Gorczykowski Anna Sokół-Łętowska Alicja Z. Kucharska |
| author_sort | Jolanta Piekarska |
| collection | DOAJ |
| description | Our experiment determined the immunotropic activity of a natural, iridoid-anthocyanin extract from honeysuckle berry (<i>Lonicera caerulea</i> L.) (LC). The extract was administered to mice infected with <i>Trichinella spiralis</i>, orally at a dose of 2 g/kg bw, six times at 24 h intervals (from day 3 prior to the infection to day 3 post-infection (dpi) with <i>T. spiralis</i>. At 5, 7, 14, and 21 dpi, samples of blood, spleen, and mesenteric lymph nodes (MLN) were collected, and isolated lymphocytes were analyzed by flow cytometry. The splenocyte proliferation was estimated with MTT testing, and the intensity of intestinal and muscle infection was also studied. LC stimulated the local immune system by inducing lymphocyte proliferation in the spleen 7 dpi and altered the percentage and absolute count of B (CD19<sup>+</sup>) and T (CD3<sup>+</sup>, CD8<sup>+</sup>) cells 7, 14, and 21 dpi in the peripheral blood. LC extract affected the dynamics of expulsion of adult <i>Trichinella</i> from the intestines and prolonged the intestinal phase of the infection but did not change the number of larvae in the muscles. These results suggest that <i>Lonicera caerulea</i> L. fruit extract modulates murine cellular immune response during intestinal phase of <i>T. spiralis</i> infection but shows no antiparasitic activity. |
| first_indexed | 2024-03-09T13:09:49Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:09:49Z |
| publishDate | 2022-03-01 |
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| series | Molecules |
| spelling | doaj.art-9cf35ede027f4f919c0177f27cb807422023-11-30T21:43:41ZengMDPI AGMolecules1420-30492022-03-01276194910.3390/molecules27061949Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in MiceJolanta Piekarska0Marianna Szczypka1Michał Gorczykowski2Anna Sokół-Łętowska3Alicja Z. Kucharska4Division of Parasitology, Department of Internal Medicine and Clinic of Horses, Dogs and Cats, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, PolandDepartment of Pharmacology and Toxicology, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, PolandDivision of Parasitology, Department of Internal Medicine and Clinic of Horses, Dogs and Cats, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, PolandOur experiment determined the immunotropic activity of a natural, iridoid-anthocyanin extract from honeysuckle berry (<i>Lonicera caerulea</i> L.) (LC). The extract was administered to mice infected with <i>Trichinella spiralis</i>, orally at a dose of 2 g/kg bw, six times at 24 h intervals (from day 3 prior to the infection to day 3 post-infection (dpi) with <i>T. spiralis</i>. At 5, 7, 14, and 21 dpi, samples of blood, spleen, and mesenteric lymph nodes (MLN) were collected, and isolated lymphocytes were analyzed by flow cytometry. The splenocyte proliferation was estimated with MTT testing, and the intensity of intestinal and muscle infection was also studied. LC stimulated the local immune system by inducing lymphocyte proliferation in the spleen 7 dpi and altered the percentage and absolute count of B (CD19<sup>+</sup>) and T (CD3<sup>+</sup>, CD8<sup>+</sup>) cells 7, 14, and 21 dpi in the peripheral blood. LC extract affected the dynamics of expulsion of adult <i>Trichinella</i> from the intestines and prolonged the intestinal phase of the infection but did not change the number of larvae in the muscles. These results suggest that <i>Lonicera caerulea</i> L. fruit extract modulates murine cellular immune response during intestinal phase of <i>T. spiralis</i> infection but shows no antiparasitic activity.https://www.mdpi.com/1420-3049/27/6/1949<i>Lonicera caerulea</i> L.<i>T. spiralis</i>immunomodulation |
| spellingShingle | Jolanta Piekarska Marianna Szczypka Michał Gorczykowski Anna Sokół-Łętowska Alicja Z. Kucharska Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice Molecules <i>Lonicera caerulea</i> L. <i>T. spiralis</i> immunomodulation |
| title | Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice |
| title_full | Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice |
| title_fullStr | Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice |
| title_full_unstemmed | Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice |
| title_short | Evaluation of Immunotropic Activity of Iridoid-Anthocyanin Extract of Honeysuckle Berries (<i>Lonicera caerulea</i> L.) in the Course of Experimental Trichinellosis in Mice |
| title_sort | evaluation of immunotropic activity of iridoid anthocyanin extract of honeysuckle berries i lonicera caerulea i l in the course of experimental trichinellosis in mice |
| topic | <i>Lonicera caerulea</i> L. <i>T. spiralis</i> immunomodulation |
| url | https://www.mdpi.com/1420-3049/27/6/1949 |
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