Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122
Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of <i>R</i><i>hizoctonia solani</i> AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/11/2254 |
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| author | Meiling Zhang Zhenrui He Xiaotong Huang Canwei Shu Erxun Zhou |
| author_facet | Meiling Zhang Zhenrui He Xiaotong Huang Canwei Shu Erxun Zhou |
| author_sort | Meiling Zhang |
| collection | DOAJ |
| description | Here, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of <i>R</i><i>hizoctonia solani</i> AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1, 1894 bp and dsRNA-2, 1755 bp), each possessing a single open reading frame (ORF). Sequence alignments and phylogenetic analyses showed that RsRV5 is a new member of the genus <i>Gammapartitivirus</i> in the family <i>Partitiviridae</i>. Transmission electron microscope (TEM) images revealed that RsRV5 has isometric viral particles with a diameter of approximately 20 nm. The mycovirus RsRV5 was successfully removed from strain D122 by using the protoplast regeneration technique, thus resulting in derivative isogenic RsRV5-cured strain D122-P being obtained. RsRV5-cured strain D122-P possessed the traits of accelerated mycelial growth rate, increased sclerotia production and enhanced pathogenicity to rice leaves compared with wild type RsRV5-infection strain D122. Transcriptome analysis showed that three genes were differentially expressed between two isogenic strains, D122 and D122-P. These findings provided new insights into the molecular mechanism of the interaction between RsRV5 and its host, D122 of <i>R. solani</i> AG-1 IA. |
| first_indexed | 2024-03-10T04:59:23Z |
| format | Article |
| id | doaj.art-9cf91af3e7a8444594475dcf8a3a11e0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T04:59:23Z |
| publishDate | 2021-11-01 |
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| series | Viruses |
| spelling | doaj.art-9cf91af3e7a8444594475dcf8a3a11e02023-11-23T01:57:37ZengMDPI AGViruses1999-49152021-11-011311225410.3390/v13112254Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122Meiling Zhang0Zhenrui He1Xiaotong Huang2Canwei Shu3Erxun Zhou4Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaHere, we describe a novel double-stranded (ds) RNA mycovirus designated Rhizoctonia solani dsRNA virus 5 (RsRV5) from strain D122 of <i>R</i><i>hizoctonia solani</i> AG-1 IA, the causal agent of rice sheath blight. The RsRV5 genome consists of two segments of dsRNA (dsRNA-1, 1894 bp and dsRNA-2, 1755 bp), each possessing a single open reading frame (ORF). Sequence alignments and phylogenetic analyses showed that RsRV5 is a new member of the genus <i>Gammapartitivirus</i> in the family <i>Partitiviridae</i>. Transmission electron microscope (TEM) images revealed that RsRV5 has isometric viral particles with a diameter of approximately 20 nm. The mycovirus RsRV5 was successfully removed from strain D122 by using the protoplast regeneration technique, thus resulting in derivative isogenic RsRV5-cured strain D122-P being obtained. RsRV5-cured strain D122-P possessed the traits of accelerated mycelial growth rate, increased sclerotia production and enhanced pathogenicity to rice leaves compared with wild type RsRV5-infection strain D122. Transcriptome analysis showed that three genes were differentially expressed between two isogenic strains, D122 and D122-P. These findings provided new insights into the molecular mechanism of the interaction between RsRV5 and its host, D122 of <i>R. solani</i> AG-1 IA.https://www.mdpi.com/1999-4915/13/11/2254mycovirus<i>Gammapartitivirus</i>hypovirulence<i>Rhizoctonia solani</i>transcriptomebiocontrol |
| spellingShingle | Meiling Zhang Zhenrui He Xiaotong Huang Canwei Shu Erxun Zhou Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122 Viruses mycovirus <i>Gammapartitivirus</i> hypovirulence <i>Rhizoctonia solani</i> transcriptome biocontrol |
| title | Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122 |
| title_full | Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122 |
| title_fullStr | Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122 |
| title_full_unstemmed | Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122 |
| title_short | Genome Organizations and Functional Analyses of a Novel Gammapartitivirus from <i>Rhizoctonia solani</i> AG-1 IA Strain D122 |
| title_sort | genome organizations and functional analyses of a novel gammapartitivirus from i rhizoctonia solani i ag 1 ia strain d122 |
| topic | mycovirus <i>Gammapartitivirus</i> hypovirulence <i>Rhizoctonia solani</i> transcriptome biocontrol |
| url | https://www.mdpi.com/1999-4915/13/11/2254 |
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