MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel
Abstract Background In this study, we evaluated the therapeutic effect of MOK, a pharmacopuncture medicine, on thyroid dysfunction in L-thyroxin (LT4)-induced hyperthyroidism rats. Methods The experimental hyperthyroidism model was prepared by the intraperitoneal injection of LT4 (0.5 mg/kg) once da...
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BMC
2017-12-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-2036-1 |
| _version_ | 1819147634385879040 |
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| author | Ji Hye Hwang Seok Yong Kang An Na Kang Hyo Won Jung Chul Jung Jin-Ho Jeong Yong-Ki Park |
| author_facet | Ji Hye Hwang Seok Yong Kang An Na Kang Hyo Won Jung Chul Jung Jin-Ho Jeong Yong-Ki Park |
| author_sort | Ji Hye Hwang |
| collection | DOAJ |
| description | Abstract Background In this study, we evaluated the therapeutic effect of MOK, a pharmacopuncture medicine, on thyroid dysfunction in L-thyroxin (LT4)-induced hyperthyroidism rats. Methods The experimental hyperthyroidism model was prepared by the intraperitoneal injection of LT4 (0.5 mg/kg) once daily for 2 weeks in SD rats. MOK extract was injected at doses of 0.3 or 3 mg/kg on acupuncture points in the thyroid glands of LT4-induced hypothyroidism rats once a day for 2 weeks. The body temperature, body weight, and food/water intake were measured once a week for 2 weeks. The levels of thyroid hormones, total cholesterol, LDL-cholesterol, GOT, and GPT were measured in the sera of rats using ELISA and an automatic blood analyzer. The histological changes of thyroid tissues were observed by H&E staining. The expression of thermo-regulating protein, TRPV1 was determined by western blot in dorsal root ganglion (DRG) and brain tissues. We also measured the contents of GSH in the liver and antioxidant enzymes, SOD, and catalase in the liver, heart, and brain tissues by enzyme-based assay and Western blot, respectively. Results The acupuncture of MOK extract on the thyroid gland of LT4-induced hyperthyroidism rats significantly decreased the body temperature, and did not change body weight and food and water intakes. MOK acupuncture significantly increased the level of TSH, and decreased the levels of T3 and T4 in hyperthyroidism rats. The expression of TRPV1 was inhibited in both DRG and brain tissues after MOK acupuncture, and the levels of GOT, GPT, total cholesterol, and LDL-cholesterol were also decreased. MOK acupuncture also inhibited the pathological feature with follicular lining epithelial thicknesses and increased follicular colloid depositions in the thyroid glands of hypothyroidism. MOK acupuncture significantly increased hepatic GSH levels and decreased the expression of SOD and catalase in the liver, heart, and brain tissues of hyperthyroidism rats. Conclusions These results suggest that the pharmacopuncture with MOK extract in hyperthyroidism can improve the pathophysiological changes through regulating the body temperature, thyroid hormones imbalance, lipid accumulation, and oxidation. This anti-hyperthyroidism effect of MOK pharmacopuncture is thought to be related to the control of thermo-regulating protein TRPV1 in DRG and brain. |
| first_indexed | 2024-12-22T13:32:56Z |
| format | Article |
| id | doaj.art-9cfc31f48db74340b5d1c52d13fcf70b |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-22T13:32:56Z |
| publishDate | 2017-12-01 |
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| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-9cfc31f48db74340b5d1c52d13fcf70b2022-12-21T18:24:07ZengBMCBMC Complementary and Alternative Medicine1472-68822017-12-0117111310.1186/s12906-017-2036-1MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channelJi Hye Hwang0Seok Yong Kang1An Na Kang2Hyo Won Jung3Chul Jung4Jin-Ho Jeong5Yong-Ki Park6Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon UniversityDepartment of Herbology, College of Korean Medicine, Dongguk UniversityDepartment of Herbology, College of Korean Medicine, Dongguk UniversityDepartment of Herbology, College of Korean Medicine, Dongguk UniversityNamsangcheon Korean Medicine ClinicJisung-Kyunghee Korean Medicine ClinicDepartment of Herbology, College of Korean Medicine, Dongguk UniversityAbstract Background In this study, we evaluated the therapeutic effect of MOK, a pharmacopuncture medicine, on thyroid dysfunction in L-thyroxin (LT4)-induced hyperthyroidism rats. Methods The experimental hyperthyroidism model was prepared by the intraperitoneal injection of LT4 (0.5 mg/kg) once daily for 2 weeks in SD rats. MOK extract was injected at doses of 0.3 or 3 mg/kg on acupuncture points in the thyroid glands of LT4-induced hypothyroidism rats once a day for 2 weeks. The body temperature, body weight, and food/water intake were measured once a week for 2 weeks. The levels of thyroid hormones, total cholesterol, LDL-cholesterol, GOT, and GPT were measured in the sera of rats using ELISA and an automatic blood analyzer. The histological changes of thyroid tissues were observed by H&E staining. The expression of thermo-regulating protein, TRPV1 was determined by western blot in dorsal root ganglion (DRG) and brain tissues. We also measured the contents of GSH in the liver and antioxidant enzymes, SOD, and catalase in the liver, heart, and brain tissues by enzyme-based assay and Western blot, respectively. Results The acupuncture of MOK extract on the thyroid gland of LT4-induced hyperthyroidism rats significantly decreased the body temperature, and did not change body weight and food and water intakes. MOK acupuncture significantly increased the level of TSH, and decreased the levels of T3 and T4 in hyperthyroidism rats. The expression of TRPV1 was inhibited in both DRG and brain tissues after MOK acupuncture, and the levels of GOT, GPT, total cholesterol, and LDL-cholesterol were also decreased. MOK acupuncture also inhibited the pathological feature with follicular lining epithelial thicknesses and increased follicular colloid depositions in the thyroid glands of hypothyroidism. MOK acupuncture significantly increased hepatic GSH levels and decreased the expression of SOD and catalase in the liver, heart, and brain tissues of hyperthyroidism rats. Conclusions These results suggest that the pharmacopuncture with MOK extract in hyperthyroidism can improve the pathophysiological changes through regulating the body temperature, thyroid hormones imbalance, lipid accumulation, and oxidation. This anti-hyperthyroidism effect of MOK pharmacopuncture is thought to be related to the control of thermo-regulating protein TRPV1 in DRG and brain.http://link.springer.com/article/10.1186/s12906-017-2036-1AntioxidationBody temperatureHyperthyroidismL-ThyroxineMOKPharmacopuncture |
| spellingShingle | Ji Hye Hwang Seok Yong Kang An Na Kang Hyo Won Jung Chul Jung Jin-Ho Jeong Yong-Ki Park MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel BMC Complementary and Alternative Medicine Antioxidation Body temperature Hyperthyroidism L-Thyroxine MOK Pharmacopuncture |
| title | MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel |
| title_full | MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel |
| title_fullStr | MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel |
| title_full_unstemmed | MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel |
| title_short | MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel |
| title_sort | mok a pharmacopuncture medicine regulates thyroid dysfunction in l thyroxin induced hyperthyroidism in rats through the regulation of oxidation and the trpv1 ion channel |
| topic | Antioxidation Body temperature Hyperthyroidism L-Thyroxine MOK Pharmacopuncture |
| url | http://link.springer.com/article/10.1186/s12906-017-2036-1 |
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