Establishment of primary keratinocyte culture from horse tissue biopsates
Primary cell lines established from skin tissue can be used in immunological, proteomic and genomic studies as in vitro skin models. The goal of our study was to establish a primary keratinocyte cell culture from tissue biopsates of two horses. The primary keratinocyte cell culture was obtained by m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2015-12-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/12696 |
| _version_ | 1797704273749868544 |
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| author | Jernej OGOREVC Tjaša LAPANJA Klavdija POKLUKAR Natalija TOMINŠEK Peter DOVČ |
| author_facet | Jernej OGOREVC Tjaša LAPANJA Klavdija POKLUKAR Natalija TOMINŠEK Peter DOVČ |
| author_sort | Jernej OGOREVC |
| collection | DOAJ |
| description | Primary cell lines established from skin tissue can be used in immunological, proteomic and genomic studies as in vitro skin models. The goal of our study was to establish a primary keratinocyte cell culture from tissue biopsates of two horses. The primary keratinocyte cell culture was obtained by mechanical and enzymatic dissociation and with explant culture method. The result was a heterogeneous primary culture comprised of keratinocytes and fibroblasts. To distinguish epithelial and mesenchymal cells immunofluorescent characterisation was performed, using antibodies against cytokeratin 14 and vimentin. We successfully at attained a primary cell line of keratinocytes, which could potentially be used to study equine skin diseases, as an animal model for human diseases, and for cosmetic and therapeutic product testing. |
| first_indexed | 2024-03-12T05:17:54Z |
| format | Article |
| id | doaj.art-9cfd7ebcf2314c56ba8157145f4ed305 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-12T05:17:54Z |
| publishDate | 2015-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-9cfd7ebcf2314c56ba8157145f4ed3052023-09-03T07:55:48ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412015-12-01106210.14720/aas.2015.106.2.319088Establishment of primary keratinocyte culture from horse tissue biopsatesJernej OGOREVC0Tjaša LAPANJA1Klavdija POKLUKAR2Natalija TOMINŠEK3Peter DOVČ4Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Study program in biotechnology, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Study program in biotechnology, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Study program in biotechnology, Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science, Groblje 3, SI-1230 Domžale, SloveniaPrimary cell lines established from skin tissue can be used in immunological, proteomic and genomic studies as in vitro skin models. The goal of our study was to establish a primary keratinocyte cell culture from tissue biopsates of two horses. The primary keratinocyte cell culture was obtained by mechanical and enzymatic dissociation and with explant culture method. The result was a heterogeneous primary culture comprised of keratinocytes and fibroblasts. To distinguish epithelial and mesenchymal cells immunofluorescent characterisation was performed, using antibodies against cytokeratin 14 and vimentin. We successfully at attained a primary cell line of keratinocytes, which could potentially be used to study equine skin diseases, as an animal model for human diseases, and for cosmetic and therapeutic product testing.https://journals.uni-lj.si/aas/article/view/12696molecular biologyprimary cell cultureskinhorseskeratinocytes |
| spellingShingle | Jernej OGOREVC Tjaša LAPANJA Klavdija POKLUKAR Natalija TOMINŠEK Peter DOVČ Establishment of primary keratinocyte culture from horse tissue biopsates Acta Agriculturae Slovenica molecular biology primary cell culture skin horses keratinocytes |
| title | Establishment of primary keratinocyte culture from horse tissue biopsates |
| title_full | Establishment of primary keratinocyte culture from horse tissue biopsates |
| title_fullStr | Establishment of primary keratinocyte culture from horse tissue biopsates |
| title_full_unstemmed | Establishment of primary keratinocyte culture from horse tissue biopsates |
| title_short | Establishment of primary keratinocyte culture from horse tissue biopsates |
| title_sort | establishment of primary keratinocyte culture from horse tissue biopsates |
| topic | molecular biology primary cell culture skin horses keratinocytes |
| url | https://journals.uni-lj.si/aas/article/view/12696 |
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