HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals
Summary: Although previous studies have reported HIV-1-specific T cell responses in HIV-1-exposed seronegative (HESN) individuals, there has been no detailed analysis of these T cells against HIV-1 infection. We investigated HIV-1-specific CD8+ T cell responses in 200 Japanese HESN men who have sex...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223021661 |
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| author | Takayuki Chikata Hiroyuki Gatanaga Hung The Nguyen Daisuke Mizushima Yu Zhang Nozomi Kuse Shinichi Oka Masafumi Takiguchi |
| author_facet | Takayuki Chikata Hiroyuki Gatanaga Hung The Nguyen Daisuke Mizushima Yu Zhang Nozomi Kuse Shinichi Oka Masafumi Takiguchi |
| author_sort | Takayuki Chikata |
| collection | DOAJ |
| description | Summary: Although previous studies have reported HIV-1-specific T cell responses in HIV-1-exposed seronegative (HESN) individuals, there has been no detailed analysis of these T cells against HIV-1 infection. We investigated HIV-1-specific CD8+ T cell responses in 200 Japanese HESN men who have sex with men (MSM). T cell responses to 143 well-characterized HIV-1 epitope peptides were analyzed by intracellular cytokine staining assay consisting of 3-week cultures of PBMCs stimulated with peptides. HLA-B∗51:01-restricted Pol TI8-specific and HLA-A∗02:06-restricted Pol SV9-specific CD8+ T cells were identified in two and one individuals, respectively, whereas CD8+ T cells specific for other HLA-A∗02:06-restricted or HLA-B∗51:01 epitopes were not present in these individuals. These epitope-specific T cells recognized HIV-1-infected cells. Because these two epitopes were previously reported to be protective in HIV-1-infected individuals, these protective epitope-specific T cells might suppress HIV-1 replication in HESN-MSM individuals. The present study suggests the contribution of protective epitope-specific T cells to protection against HIV-1 infection. |
| first_indexed | 2024-03-11T18:25:15Z |
| format | Article |
| id | doaj.art-9d0278eeea65406abac5d59c7e60ba5b |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T18:25:15Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9d0278eeea65406abac5d59c7e60ba5b2023-10-14T04:45:10ZengElsevieriScience2589-00422023-11-012611108089HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individualsTakayuki Chikata0Hiroyuki Gatanaga1Hung The Nguyen2Daisuke Mizushima3Yu Zhang4Nozomi Kuse5Shinichi Oka6Masafumi Takiguchi7Tokyo Laboratory and Division of International Collaboration Research, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 162-0052, Japan; Corresponding authorAIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, JapanTokyo Laboratory and Division of International Collaboration Research, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 162-0052, JapanAIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, JapanTokyo Laboratory and Division of International Collaboration Research, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 162-0052, JapanTokyo Laboratory and Division of International Collaboration Research, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 162-0052, JapanAIDS Clinical Center, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, JapanTokyo Laboratory and Division of International Collaboration Research, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 162-0052, Japan; Corresponding authorSummary: Although previous studies have reported HIV-1-specific T cell responses in HIV-1-exposed seronegative (HESN) individuals, there has been no detailed analysis of these T cells against HIV-1 infection. We investigated HIV-1-specific CD8+ T cell responses in 200 Japanese HESN men who have sex with men (MSM). T cell responses to 143 well-characterized HIV-1 epitope peptides were analyzed by intracellular cytokine staining assay consisting of 3-week cultures of PBMCs stimulated with peptides. HLA-B∗51:01-restricted Pol TI8-specific and HLA-A∗02:06-restricted Pol SV9-specific CD8+ T cells were identified in two and one individuals, respectively, whereas CD8+ T cells specific for other HLA-A∗02:06-restricted or HLA-B∗51:01 epitopes were not present in these individuals. These epitope-specific T cells recognized HIV-1-infected cells. Because these two epitopes were previously reported to be protective in HIV-1-infected individuals, these protective epitope-specific T cells might suppress HIV-1 replication in HESN-MSM individuals. The present study suggests the contribution of protective epitope-specific T cells to protection against HIV-1 infection.http://www.sciencedirect.com/science/article/pii/S2589004223021661Immune responseCell biology |
| spellingShingle | Takayuki Chikata Hiroyuki Gatanaga Hung The Nguyen Daisuke Mizushima Yu Zhang Nozomi Kuse Shinichi Oka Masafumi Takiguchi HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals iScience Immune response Cell biology |
| title | HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals |
| title_full | HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals |
| title_fullStr | HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals |
| title_full_unstemmed | HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals |
| title_short | HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals |
| title_sort | hiv 1 protective epitope specific cd8 t cells in hiv 1 exposed seronegative individuals |
| topic | Immune response Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223021661 |
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