Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment
The genus Streptomyces under phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated as DST103 isolated from a wetland fresh water sediment of Tamdil Lake, Mizoram, Northeast, India was identified as S...
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Frontiers Media S.A.
2017-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2017.00068/full |
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| author | Zothanpuia Ajit K. Passari Preeti Chandra Vincent V. Leo Vineet K. Mishra Brijesh Kumar Bhim P. Singh |
| author_facet | Zothanpuia Ajit K. Passari Preeti Chandra Vincent V. Leo Vineet K. Mishra Brijesh Kumar Bhim P. Singh |
| author_sort | Zothanpuia |
| collection | DOAJ |
| description | The genus Streptomyces under phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated as DST103 isolated from a wetland fresh water sediment of Tamdil Lake, Mizoram, Northeast, India was identified as Streptomyces cyaneofuscatus (KY287599) using 16SrRNA gene sequencing which shares 99.87% sequence similarity with Streptomyces cyaneofuscatus NRRL B-2570T. The strain showed broad spectrum antimicrobial activities against Gram negative bacteria (Escherichia coli MTCC 739 and Pseudomonas aeruginosa MTCC 2453), Gram positive bacteria (Micrococcus luteus NCIM 2170 and Staphylococcus aureus MTCC 96) and yeast pathogen Candida albicans MTCC 3017). The methanolic extract of the strain DST103 exhibited highest antimicrobial activity against E. coli (IC50 = 2.10 μg/mL) and minimum activity against S. aureus (IC50 = 43.63 μg/mL). Five antibiotics [trimethoprim (18 μg/g), fluconazole (6 μg/g), ketoconazole (18 μg/g), nalidixic acid (135 μg/g), and rifampicin (56 μg/g)] were detected and quantified using ultra-performance liquid chromatography (UPLC-ESI-MS/MS). Further, biosynthetic potential genes [polyketide synthases type II, non-ribosomal peptide synthetases, and aminodeoxyisochorismate synthase (phzE)] were also detected in strain DST103 which may possibly be responsible for the production of antimicrobial compounds. Additionally, gas chromatography-mass spectrometry analysis showed the presence of four volatile compounds which might be responsible for their diverse biological activity. The present study revealed the presence of bioactive compounds in strain DST103, which may be a promising resource for the discovery of novel bioactive metabolites against wide range of pathogens. |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T12:24:37Z |
| publishDate | 2017-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-9d02bcc12045499e8da017c33f7a0d152022-12-22T03:33:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-01-01810.3389/fmicb.2017.00068235521Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment Zothanpuia0Ajit K. Passari1Preeti Chandra2Vincent V. Leo3Vineet K. Mishra4Brijesh Kumar5Bhim P. Singh6Molecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram UniversityAizawl, IndiaMolecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram UniversityAizawl, IndiaCSIR-Central Drug Research Institute, Sophisticated Analytical Instrument FacilityLucknow, IndiaMolecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram UniversityAizawl, IndiaMolecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram UniversityAizawl, IndiaCSIR-Central Drug Research Institute, Sophisticated Analytical Instrument FacilityLucknow, IndiaMolecular Microbiology and Systematics Laboratory, Department of Biotechnology, Mizoram UniversityAizawl, IndiaThe genus Streptomyces under phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated as DST103 isolated from a wetland fresh water sediment of Tamdil Lake, Mizoram, Northeast, India was identified as Streptomyces cyaneofuscatus (KY287599) using 16SrRNA gene sequencing which shares 99.87% sequence similarity with Streptomyces cyaneofuscatus NRRL B-2570T. The strain showed broad spectrum antimicrobial activities against Gram negative bacteria (Escherichia coli MTCC 739 and Pseudomonas aeruginosa MTCC 2453), Gram positive bacteria (Micrococcus luteus NCIM 2170 and Staphylococcus aureus MTCC 96) and yeast pathogen Candida albicans MTCC 3017). The methanolic extract of the strain DST103 exhibited highest antimicrobial activity against E. coli (IC50 = 2.10 μg/mL) and minimum activity against S. aureus (IC50 = 43.63 μg/mL). Five antibiotics [trimethoprim (18 μg/g), fluconazole (6 μg/g), ketoconazole (18 μg/g), nalidixic acid (135 μg/g), and rifampicin (56 μg/g)] were detected and quantified using ultra-performance liquid chromatography (UPLC-ESI-MS/MS). Further, biosynthetic potential genes [polyketide synthases type II, non-ribosomal peptide synthetases, and aminodeoxyisochorismate synthase (phzE)] were also detected in strain DST103 which may possibly be responsible for the production of antimicrobial compounds. Additionally, gas chromatography-mass spectrometry analysis showed the presence of four volatile compounds which might be responsible for their diverse biological activity. The present study revealed the presence of bioactive compounds in strain DST103, which may be a promising resource for the discovery of novel bioactive metabolites against wide range of pathogens.https://www.frontiersin.org/article/10.3389/fmicb.2017.00068/fullStreptomyces cyaneofuscatusantimicrobial activitybiosynthetic genesGC-MSUPLC-ESI-MS/MS |
| spellingShingle | Zothanpuia Ajit K. Passari Preeti Chandra Vincent V. Leo Vineet K. Mishra Brijesh Kumar Bhim P. Singh Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment Frontiers in Microbiology Streptomyces cyaneofuscatus antimicrobial activity biosynthetic genes GC-MS UPLC-ESI-MS/MS |
| title | Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment |
| title_full | Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment |
| title_fullStr | Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment |
| title_full_unstemmed | Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment |
| title_short | Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment |
| title_sort | production of potent antimicrobial compounds from streptomyces cyaneofuscatus associated with fresh water sediment |
| topic | Streptomyces cyaneofuscatus antimicrobial activity biosynthetic genes GC-MS UPLC-ESI-MS/MS |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2017.00068/full |
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