Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it>
<p>Abstract</p> <p>Background</p> <p>Carboxyl/cholinesterases (CCEs) have pivotal roles in dietary detoxification, pheromone or hormone degradation and neurodevelopment. The recent completion of genome projects in various insect species has led to the identification of...
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| Format: | Article |
| Language: | English |
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BMC
2010-06-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/377 |
| _version_ | 1818525986881273856 |
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| author | Shiotsuki Takahiro Tsubota Takuya |
| author_facet | Shiotsuki Takahiro Tsubota Takuya |
| author_sort | Shiotsuki Takahiro |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Carboxyl/cholinesterases (CCEs) have pivotal roles in dietary detoxification, pheromone or hormone degradation and neurodevelopment. The recent completion of genome projects in various insect species has led to the identification of multiple CCEs with unknown functions. Here, we analyzed the phylogeny, expression and genomic distribution of 69 putative CCEs in the silkworm, <it>Bombyx </it><it>mori </it>(Lepidoptera: Bombycidae).</p> <p>Results</p> <p>A phylogenetic tree of CCEs in <it>B. mori </it>and other lepidopteran species was constructed. The expression pattern of each <it>B. mori </it>CCE was also investigated by a search of an expressed sequence tag (EST) database, and the relationship between phylogeny and expression was analyzed. A large number of <it>B. mori </it>CCEs were identified from a midgut EST library. CCEs expressed in the midgut formed a cluster in the phylogenetic tree that included not only <it>B. mori </it>genes but also those of other lepidopteran species. The silkworm, and possibly also other lepidopteran species, has a large number of CCEs, and this might be a consequence of the large cluster of midgut CCEs. Investigation of intron-exon organization in <it>B. mori </it>CCEs revealed that their positions and splicing site phases were strongly conserved. Several <it>B. mori </it>CCEs, including juvenile hormone esterase, not only showed clustering in the phylogenetic tree but were also closely located on silkworm chromosomes. We investigated the phylogeny and microsynteny of neuroligins in detail, among many CCEs. Interestingly, we found the evolution of this gene appeared not to be conserved between <it>B. mori </it>and other insect orders.</p> <p>Conclusions</p> <p>We analyzed 69 putative CCEs from <it>B. mori</it>. Comparison of these CCEs with other lepidopteran CCEs indicated that they had conserved expression and function in this insect order. The analyses showed that CCEs were unevenly distributed across the genome of <it>B. mori </it>and suggested that neuroligins may have a distinct evolutionary history from other insect order. It is possible that such an uneven genomic distribution and a unique neuroligin evolution are shared with other lepidopteran insects. Our genomic analysis has provided novel information on the CCEs of the silkworm, which will be of value to understanding the biology, physiology and evolution of insect CCEs.</p> |
| first_indexed | 2024-12-11T06:16:40Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T06:16:40Z |
| publishDate | 2010-06-01 |
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| spelling | doaj.art-9d02cceb0d9a4e8eb3c812ea5cbb478e2022-12-22T01:17:58ZengBMCBMC Genomics1471-21642010-06-0111137710.1186/1471-2164-11-377Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it>Shiotsuki TakahiroTsubota Takuya<p>Abstract</p> <p>Background</p> <p>Carboxyl/cholinesterases (CCEs) have pivotal roles in dietary detoxification, pheromone or hormone degradation and neurodevelopment. The recent completion of genome projects in various insect species has led to the identification of multiple CCEs with unknown functions. Here, we analyzed the phylogeny, expression and genomic distribution of 69 putative CCEs in the silkworm, <it>Bombyx </it><it>mori </it>(Lepidoptera: Bombycidae).</p> <p>Results</p> <p>A phylogenetic tree of CCEs in <it>B. mori </it>and other lepidopteran species was constructed. The expression pattern of each <it>B. mori </it>CCE was also investigated by a search of an expressed sequence tag (EST) database, and the relationship between phylogeny and expression was analyzed. A large number of <it>B. mori </it>CCEs were identified from a midgut EST library. CCEs expressed in the midgut formed a cluster in the phylogenetic tree that included not only <it>B. mori </it>genes but also those of other lepidopteran species. The silkworm, and possibly also other lepidopteran species, has a large number of CCEs, and this might be a consequence of the large cluster of midgut CCEs. Investigation of intron-exon organization in <it>B. mori </it>CCEs revealed that their positions and splicing site phases were strongly conserved. Several <it>B. mori </it>CCEs, including juvenile hormone esterase, not only showed clustering in the phylogenetic tree but were also closely located on silkworm chromosomes. We investigated the phylogeny and microsynteny of neuroligins in detail, among many CCEs. Interestingly, we found the evolution of this gene appeared not to be conserved between <it>B. mori </it>and other insect orders.</p> <p>Conclusions</p> <p>We analyzed 69 putative CCEs from <it>B. mori</it>. Comparison of these CCEs with other lepidopteran CCEs indicated that they had conserved expression and function in this insect order. The analyses showed that CCEs were unevenly distributed across the genome of <it>B. mori </it>and suggested that neuroligins may have a distinct evolutionary history from other insect order. It is possible that such an uneven genomic distribution and a unique neuroligin evolution are shared with other lepidopteran insects. Our genomic analysis has provided novel information on the CCEs of the silkworm, which will be of value to understanding the biology, physiology and evolution of insect CCEs.</p>http://www.biomedcentral.com/1471-2164/11/377 |
| spellingShingle | Shiotsuki Takahiro Tsubota Takuya Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it> BMC Genomics |
| title | Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it> |
| title_full | Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it> |
| title_fullStr | Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it> |
| title_full_unstemmed | Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it> |
| title_short | Genomic analysis of carboxyl/cholinesterase genes in the silkworm <it>Bombyx mori</it> |
| title_sort | genomic analysis of carboxyl cholinesterase genes in the silkworm it bombyx mori it |
| url | http://www.biomedcentral.com/1471-2164/11/377 |
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