Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice
Summary: Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdO...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002069 |
| _version_ | 1818719785541697536 |
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| author | Sigrid Fra-Bido Simon A. Walker Silvia Innocentin Michelle A. Linterman |
| author_facet | Sigrid Fra-Bido Simon A. Walker Silvia Innocentin Michelle A. Linterman |
| author_sort | Sigrid Fra-Bido |
| collection | DOAJ |
| description | Summary: Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues.For complete information on the generation and use of this protocol to examine immune responses to the COVID vaccine ChAdOx1 nCoV-19, please refer to Silva-Cayetano et al. (2020). |
| first_indexed | 2024-12-17T20:12:28Z |
| format | Article |
| id | doaj.art-9d0338dc429347a3808a14fbe1d9621e |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T20:12:28Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-9d0338dc429347a3808a14fbe1d9621e2022-12-21T21:34:13ZengElsevierSTAR Protocols2666-16672021-09-0123100499Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated miceSigrid Fra-Bido0Simon A. Walker1Silvia Innocentin2Michelle A. Linterman3The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK; Corresponding authorThe Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UKThe Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UKThe Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, UK; Corresponding authorSummary: Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues.For complete information on the generation and use of this protocol to examine immune responses to the COVID vaccine ChAdOx1 nCoV-19, please refer to Silva-Cayetano et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721002069AntibodyCell BiologyImmunologyMicrobiologyMicroscopyModel Organisms |
| spellingShingle | Sigrid Fra-Bido Simon A. Walker Silvia Innocentin Michelle A. Linterman Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice STAR Protocols Antibody Cell Biology Immunology Microbiology Microscopy Model Organisms |
| title | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| title_full | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| title_fullStr | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| title_full_unstemmed | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| title_short | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| title_sort | optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
| topic | Antibody Cell Biology Immunology Microbiology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721002069 |
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