Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species
Abstract Background Vibrio cholerae, the causative agent of cholera, is a well-studied species, whereas Vibrio metoecus is a recently described close relative that is also associated with human infections. The availability of V. metoecus genomes provides further insight into its genetic differences...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13099-022-00516-x |
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| author | Fabini D. Orata Nora A. S. Hussain Kevin Y. H. Liang Dalong Hu Yann F. Boucher |
| author_facet | Fabini D. Orata Nora A. S. Hussain Kevin Y. H. Liang Dalong Hu Yann F. Boucher |
| author_sort | Fabini D. Orata |
| collection | DOAJ |
| description | Abstract Background Vibrio cholerae, the causative agent of cholera, is a well-studied species, whereas Vibrio metoecus is a recently described close relative that is also associated with human infections. The availability of V. metoecus genomes provides further insight into its genetic differences from V. cholerae. Additionally, both species have been co-isolated from a cholera-free brackish coastal pond and have been suggested to interact with each other by horizontal gene transfer (HGT). Results The genomes of 17 strains from each species were sequenced. All strains share a large core genome (2675 gene families) and very few genes are unique to each species (< 3% of the pan-genome of both species). This led to the identification of potential molecular markers—for nitrite reduction, as well as peptidase and rhodanese activities—to further distinguish V. metoecus from V. cholerae. Interspecies HGT events were inferred in 21% of the core genes and 45% of the accessory genes. A directional bias in gene transfer events was found in the core genome, where V. metoecus was a recipient of three times (75%) more genes from V. cholerae than it was a donor (25%). Conclusion V. metoecus was misclassified as an atypical variant of V. cholerae due to their resemblance in a majority of biochemical characteristics. More distinguishing phenotypic assays can be developed based on the discovery of potential gene markers to avoid any future misclassifications. Furthermore, differences in relative abundance or seasonality were observed between the species and could contribute to the bias in directionality of HGT. |
| first_indexed | 2024-04-13T08:07:11Z |
| format | Article |
| id | doaj.art-9d097fb6a7054925a2517d0c3928967f |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-04-13T08:07:11Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-9d097fb6a7054925a2517d0c3928967f2022-12-22T02:55:07ZengBMCGut Pathogens1757-47492022-11-0114111210.1186/s13099-022-00516-xGenomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related speciesFabini D. Orata0Nora A. S. Hussain1Kevin Y. H. Liang2Dalong Hu3Yann F. Boucher4Department of Biological Sciences, University of AlbertaDepartment of Biological Sciences, University of AlbertaDepartment of Biological Sciences, University of AlbertaSaw Swee Hock School of Public Health, National University of Singapore and National University Hospital SystemDepartment of Biological Sciences, University of AlbertaAbstract Background Vibrio cholerae, the causative agent of cholera, is a well-studied species, whereas Vibrio metoecus is a recently described close relative that is also associated with human infections. The availability of V. metoecus genomes provides further insight into its genetic differences from V. cholerae. Additionally, both species have been co-isolated from a cholera-free brackish coastal pond and have been suggested to interact with each other by horizontal gene transfer (HGT). Results The genomes of 17 strains from each species were sequenced. All strains share a large core genome (2675 gene families) and very few genes are unique to each species (< 3% of the pan-genome of both species). This led to the identification of potential molecular markers—for nitrite reduction, as well as peptidase and rhodanese activities—to further distinguish V. metoecus from V. cholerae. Interspecies HGT events were inferred in 21% of the core genes and 45% of the accessory genes. A directional bias in gene transfer events was found in the core genome, where V. metoecus was a recipient of three times (75%) more genes from V. cholerae than it was a donor (25%). Conclusion V. metoecus was misclassified as an atypical variant of V. cholerae due to their resemblance in a majority of biochemical characteristics. More distinguishing phenotypic assays can be developed based on the discovery of potential gene markers to avoid any future misclassifications. Furthermore, differences in relative abundance or seasonality were observed between the species and could contribute to the bias in directionality of HGT.https://doi.org/10.1186/s13099-022-00516-xVibrio choleraeVibrio metoecusComparative genomicsHorizontal gene transferEvolution |
| spellingShingle | Fabini D. Orata Nora A. S. Hussain Kevin Y. H. Liang Dalong Hu Yann F. Boucher Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species Gut Pathogens Vibrio cholerae Vibrio metoecus Comparative genomics Horizontal gene transfer Evolution |
| title | Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species |
| title_full | Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species |
| title_fullStr | Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species |
| title_full_unstemmed | Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species |
| title_short | Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species |
| title_sort | genomes of vibrio metoecus co isolated with vibrio cholerae extend our understanding of differences between these closely related species |
| topic | Vibrio cholerae Vibrio metoecus Comparative genomics Horizontal gene transfer Evolution |
| url | https://doi.org/10.1186/s13099-022-00516-x |
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