Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model
An increasing body of research has demonstrated the significant role of long non-coding RNAs (lncRNAs) in the pathogenesis of stroke. They can actively contribute to the disease’s progression either by directly participating in its pathogenesis or by acting as mediators through competing endogenous...
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MDPI AG
2024-03-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/17/3/384 |
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| author | Chao Zhang Junpeng Gao Dan Xiong Yan Zhao |
| author_facet | Chao Zhang Junpeng Gao Dan Xiong Yan Zhao |
| author_sort | Chao Zhang |
| collection | DOAJ |
| description | An increasing body of research has demonstrated the significant role of long non-coding RNAs (lncRNAs) in the pathogenesis of stroke. They can actively contribute to the disease’s progression either by directly participating in its pathogenesis or by acting as mediators through competing endogenous RNA (ceRNA) mechanisms. Concurrently, epigenetics plays a pivotal role in the pathological mechanisms underlying stroke. Epigenetic factors serve as valuable markers for disease progression, diagnostic biomarkers, and novel therapeutic targets. One of the most prevalent epigenetic modifications is 5-methylcytosine (m<sup>5</sup>C). However, the specific profiles of 5-methylcytosine in lncRNAs associated with stroke remain to be solved. Within the scope of this research, we performed a thorough transcriptome-wide analysis of m<sup>5</sup>C methylation within lncRNAs by methylated RNA immunoprecipitation sequencing (MeRIP-Seq), within a mouse stroke model induced by middle cerebral artery occlusion. Our findings reveal substantial disparities in both the quantity and distribution of m<sup>5</sup>C within the mouse stroke model compared to normal mice. This suggests a potential linkage between stroke and lncRNA m<sup>5</sup>C modifications, offering valuable insights into the mechanisms of stroke pathogenesis and the development of new drug targets. |
| first_indexed | 2024-04-24T17:55:16Z |
| format | Article |
| id | doaj.art-9d0be827411144bc8fb429823acdbc0d |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-04-24T17:55:16Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-9d0be827411144bc8fb429823acdbc0d2024-03-27T13:59:27ZengMDPI AGPharmaceuticals1424-82472024-03-0117338410.3390/ph17030384Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia ModelChao Zhang0Junpeng Gao1Dan Xiong2Yan Zhao3Emergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaEmergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaEmergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaEmergency Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaAn increasing body of research has demonstrated the significant role of long non-coding RNAs (lncRNAs) in the pathogenesis of stroke. They can actively contribute to the disease’s progression either by directly participating in its pathogenesis or by acting as mediators through competing endogenous RNA (ceRNA) mechanisms. Concurrently, epigenetics plays a pivotal role in the pathological mechanisms underlying stroke. Epigenetic factors serve as valuable markers for disease progression, diagnostic biomarkers, and novel therapeutic targets. One of the most prevalent epigenetic modifications is 5-methylcytosine (m<sup>5</sup>C). However, the specific profiles of 5-methylcytosine in lncRNAs associated with stroke remain to be solved. Within the scope of this research, we performed a thorough transcriptome-wide analysis of m<sup>5</sup>C methylation within lncRNAs by methylated RNA immunoprecipitation sequencing (MeRIP-Seq), within a mouse stroke model induced by middle cerebral artery occlusion. Our findings reveal substantial disparities in both the quantity and distribution of m<sup>5</sup>C within the mouse stroke model compared to normal mice. This suggests a potential linkage between stroke and lncRNA m<sup>5</sup>C modifications, offering valuable insights into the mechanisms of stroke pathogenesis and the development of new drug targets.https://www.mdpi.com/1424-8247/17/3/384lncRNAs5-methylcytosinestrokemiddle cerebral artery occlusionMeRIP-seq |
| spellingShingle | Chao Zhang Junpeng Gao Dan Xiong Yan Zhao Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model Pharmaceuticals lncRNAs 5-methylcytosine stroke middle cerebral artery occlusion MeRIP-seq |
| title | Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model |
| title_full | Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model |
| title_fullStr | Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model |
| title_full_unstemmed | Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model |
| title_short | Transcriptome-Wide 5-Methylcytosine Profiling of lncRNAs in the Mouse Cerebral Ischemia Model |
| title_sort | transcriptome wide 5 methylcytosine profiling of lncrnas in the mouse cerebral ischemia model |
| topic | lncRNAs 5-methylcytosine stroke middle cerebral artery occlusion MeRIP-seq |
| url | https://www.mdpi.com/1424-8247/17/3/384 |
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