GbFLSa overexpression negatively regulates proanthocyanin biosynthesis
Flavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length ging...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1093656/full |
| _version_ | 1797924520476016640 |
|---|---|
| author | Jing Guo Yaqiong Wu Yaqiong Wu Yaqiong Wu Tongli Wang Yue Xin Guibin Wang Qi Zhou Qi Zhou Li-An Xu |
| author_facet | Jing Guo Yaqiong Wu Yaqiong Wu Yaqiong Wu Tongli Wang Yue Xin Guibin Wang Qi Zhou Qi Zhou Li-An Xu |
| author_sort | Jing Guo |
| collection | DOAJ |
| description | Flavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length gingko GbFLSa gene (1314 bp), which encodes a 363 amino acid protein that has a typical 2-oxoglutarate (2OG)-Fe(II) oxygenase region. Recombinant GbFLSa protein with a molecular mass of 41 kDa was expressed in Escherichia coli BL21(DE3). The protein was localized to the cytoplasm. Moreover, proanthocyanins, including catechin, epicatechin, epigallocatechin and gallocatechin, were significantly less abundant in transgenic poplar than in nontransgenic (CK) plants. In addition, dihydroflavonol 4-reductase, anthocyanidin synthase and leucoanthocyanidin reductase expression levels were significantly lower than those of their CK counterparts. GbFLSa thus encodes a functional protein that might negatively regulate proanthocyanin biosynthesis. This study helps elucidate the role of GbFLSa in plant metabolism and the potential molecular mechanism of flavonoid biosynthesis. |
| first_indexed | 2024-04-10T15:02:14Z |
| format | Article |
| id | doaj.art-9d0d93ea16784f7392493836257d263e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T15:02:14Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9d0d93ea16784f7392493836257d263e2023-02-15T09:46:59ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.10936561093656GbFLSa overexpression negatively regulates proanthocyanin biosynthesisJing Guo0Yaqiong Wu1Yaqiong Wu2Yaqiong Wu3Tongli Wang4Yue Xin5Guibin Wang6Qi Zhou7Qi Zhou8Li-An Xu9Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, ChinaDepartment of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaDepartment of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaForest Breeding Institute, Zhejiang Academy of Forestry, Hangzhou, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, ChinaFlavonoids are important secondary metabolites with extensive pharmacological functions. Ginkgo biloba L. (ginkgo) has attracted extensive attention because of its high flavonoid medicinal value. However, little is understood about ginkgo flavonol biosynthesis. Herein, we cloned the full-length gingko GbFLSa gene (1314 bp), which encodes a 363 amino acid protein that has a typical 2-oxoglutarate (2OG)-Fe(II) oxygenase region. Recombinant GbFLSa protein with a molecular mass of 41 kDa was expressed in Escherichia coli BL21(DE3). The protein was localized to the cytoplasm. Moreover, proanthocyanins, including catechin, epicatechin, epigallocatechin and gallocatechin, were significantly less abundant in transgenic poplar than in nontransgenic (CK) plants. In addition, dihydroflavonol 4-reductase, anthocyanidin synthase and leucoanthocyanidin reductase expression levels were significantly lower than those of their CK counterparts. GbFLSa thus encodes a functional protein that might negatively regulate proanthocyanin biosynthesis. This study helps elucidate the role of GbFLSa in plant metabolism and the potential molecular mechanism of flavonoid biosynthesis.https://www.frontiersin.org/articles/10.3389/fpls.2023.1093656/fullflavonol synthasegene expressionflavonoid biosynthesistransgenic poplarmetabolite |
| spellingShingle | Jing Guo Yaqiong Wu Yaqiong Wu Yaqiong Wu Tongli Wang Yue Xin Guibin Wang Qi Zhou Qi Zhou Li-An Xu GbFLSa overexpression negatively regulates proanthocyanin biosynthesis Frontiers in Plant Science flavonol synthase gene expression flavonoid biosynthesis transgenic poplar metabolite |
| title | GbFLSa overexpression negatively regulates proanthocyanin biosynthesis |
| title_full | GbFLSa overexpression negatively regulates proanthocyanin biosynthesis |
| title_fullStr | GbFLSa overexpression negatively regulates proanthocyanin biosynthesis |
| title_full_unstemmed | GbFLSa overexpression negatively regulates proanthocyanin biosynthesis |
| title_short | GbFLSa overexpression negatively regulates proanthocyanin biosynthesis |
| title_sort | gbflsa overexpression negatively regulates proanthocyanin biosynthesis |
| topic | flavonol synthase gene expression flavonoid biosynthesis transgenic poplar metabolite |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1093656/full |
| work_keys_str_mv | AT jingguo gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT yaqiongwu gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT yaqiongwu gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT yaqiongwu gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT tongliwang gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT yuexin gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT guibinwang gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT qizhou gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT qizhou gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis AT lianxu gbflsaoverexpressionnegativelyregulatesproanthocyaninbiosynthesis |