Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis
There is a great need for research aimed at understanding drought tolerance, screening for drought tolerant varieties and breeding cops with an improved water use efficiency. Bananas and plantains are a major staple food and export product with a worldwide production of over 135 million tonnes per y...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2012-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00176/full |
| _version_ | 1817968008251834368 |
|---|---|
| author | Anne-Catherine eVanhove Wesley eVermaelen Bart ePanis Rony eSwennen Sebastien eCarpentier Sebastien eCarpentier |
| author_facet | Anne-Catherine eVanhove Wesley eVermaelen Bart ePanis Rony eSwennen Sebastien eCarpentier Sebastien eCarpentier |
| author_sort | Anne-Catherine eVanhove |
| collection | DOAJ |
| description | There is a great need for research aimed at understanding drought tolerance, screening for drought tolerant varieties and breeding cops with an improved water use efficiency. Bananas and plantains are a major staple food and export product with a worldwide production of over 135 million tonnes per year. Water however is the most limiting abiotic factor in banana production. A screening of the Musa biodiversity has not yet been performed. We at KU Leuven host the Musa International Germplasm collection with over 1200 accessions. To screen the Musa biodiversity for drought tolerant varieties, we developed a screening test for in vitro plants. Five varieties representing different genomic constitutions in banana (AAAh, AAA, AAB, AABp and ABB) were selected and subjected to a mild osmotic stress. The ABB variety showed the smallest stress induced growth reduction. To get an insight into the acclimation and the accomplishment of homeostasis, the leaf proteome of this variety was characterized via 2D DIGE. After extraction of the leaf proteome of 6 control and 6 stressed plants, 2600 spots could be distinguished. A PCA analysis indicates that control and stressed plants can blindly be classified based on their proteome. One hundred and twelve proteins were significantly more abundant in the stressed plants and eighteen proteins were significantly more abundant in control plants (FDR α 0.05). Twenty four differential proteins could be identified. The proteome analysis clearly shows that there is a new balance in the stressed plants and that the respiration, metabolism of ROS and several dehydrogenases involved in NAD/NADH homeostasis play an important role. |
| first_indexed | 2024-04-13T20:02:18Z |
| format | Article |
| id | doaj.art-9d0e4fd64b1441fd9a0c523e98f51a7f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T20:02:18Z |
| publishDate | 2012-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9d0e4fd64b1441fd9a0c523e98f51a7f2022-12-22T02:32:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2012-08-01310.3389/fpls.2012.0017631251Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasisAnne-Catherine eVanhove0Wesley eVermaelen1Bart ePanis2Rony eSwennen3Sebastien eCarpentier4Sebastien eCarpentier5KULeuvenKULeuvenKULeuvenKULeuvenKULeuvenKULeuvenThere is a great need for research aimed at understanding drought tolerance, screening for drought tolerant varieties and breeding cops with an improved water use efficiency. Bananas and plantains are a major staple food and export product with a worldwide production of over 135 million tonnes per year. Water however is the most limiting abiotic factor in banana production. A screening of the Musa biodiversity has not yet been performed. We at KU Leuven host the Musa International Germplasm collection with over 1200 accessions. To screen the Musa biodiversity for drought tolerant varieties, we developed a screening test for in vitro plants. Five varieties representing different genomic constitutions in banana (AAAh, AAA, AAB, AABp and ABB) were selected and subjected to a mild osmotic stress. The ABB variety showed the smallest stress induced growth reduction. To get an insight into the acclimation and the accomplishment of homeostasis, the leaf proteome of this variety was characterized via 2D DIGE. After extraction of the leaf proteome of 6 control and 6 stressed plants, 2600 spots could be distinguished. A PCA analysis indicates that control and stressed plants can blindly be classified based on their proteome. One hundred and twelve proteins were significantly more abundant in the stressed plants and eighteen proteins were significantly more abundant in control plants (FDR α 0.05). Twenty four differential proteins could be identified. The proteome analysis clearly shows that there is a new balance in the stressed plants and that the respiration, metabolism of ROS and several dehydrogenases involved in NAD/NADH homeostasis play an important role.http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00176/fullProteomicsReactive Oxygen SpeciesBiodiversitydrought tolerancegrowth modeling |
| spellingShingle | Anne-Catherine eVanhove Wesley eVermaelen Bart ePanis Rony eSwennen Sebastien eCarpentier Sebastien eCarpentier Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis Frontiers in Plant Science Proteomics Reactive Oxygen Species Biodiversity drought tolerance growth modeling |
| title | Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis |
| title_full | Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis |
| title_fullStr | Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis |
| title_full_unstemmed | Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis |
| title_short | Screening the banana biodiversity for drought tolerance: can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis |
| title_sort | screening the banana biodiversity for drought tolerance can an in vitro growth model and proteomics be used as a tool to discover tolerant varieties and understand homeostasis |
| topic | Proteomics Reactive Oxygen Species Biodiversity drought tolerance growth modeling |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2012.00176/full |
| work_keys_str_mv | AT annecatherineevanhove screeningthebananabiodiversityfordroughttolerancecananinvitrogrowthmodelandproteomicsbeusedasatooltodiscovertolerantvarietiesandunderstandhomeostasis AT wesleyevermaelen screeningthebananabiodiversityfordroughttolerancecananinvitrogrowthmodelandproteomicsbeusedasatooltodiscovertolerantvarietiesandunderstandhomeostasis AT bartepanis screeningthebananabiodiversityfordroughttolerancecananinvitrogrowthmodelandproteomicsbeusedasatooltodiscovertolerantvarietiesandunderstandhomeostasis AT ronyeswennen screeningthebananabiodiversityfordroughttolerancecananinvitrogrowthmodelandproteomicsbeusedasatooltodiscovertolerantvarietiesandunderstandhomeostasis AT sebastienecarpentier screeningthebananabiodiversityfordroughttolerancecananinvitrogrowthmodelandproteomicsbeusedasatooltodiscovertolerantvarietiesandunderstandhomeostasis AT sebastienecarpentier screeningthebananabiodiversityfordroughttolerancecananinvitrogrowthmodelandproteomicsbeusedasatooltodiscovertolerantvarietiesandunderstandhomeostasis |