The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study
Introduction: Serum biomarkers, such as Neurofilament Light (NF-L), Glial Fibrillary Acidic Protein (GFAP), Ubiquitin C-terminal Hydrolase (UCH-L1), and Total-tau (T-Tau) have been proposed for outcome prediction in the acute phase of severe traumatic brain injury, but they have been less investigat...
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MDPI AG
2024-02-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/14/3/239 |
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| author | Rita Formisano Mariagrazia D’Ippolito Marco Giustini Sheila Catani Stefania Mondello Iliana Piccolino Filomena Iannuzzi Kevin K. Wang Ronald L. Hayes |
| author_facet | Rita Formisano Mariagrazia D’Ippolito Marco Giustini Sheila Catani Stefania Mondello Iliana Piccolino Filomena Iannuzzi Kevin K. Wang Ronald L. Hayes |
| author_sort | Rita Formisano |
| collection | DOAJ |
| description | Introduction: Serum biomarkers, such as Neurofilament Light (NF-L), Glial Fibrillary Acidic Protein (GFAP), Ubiquitin C-terminal Hydrolase (UCH-L1), and Total-tau (T-Tau) have been proposed for outcome prediction in the acute phase of severe traumatic brain injury, but they have been less investigated in patients with prolonged DoC (p-DoC). Methods: We enrolled 25 p-DoC patients according to the Coma Recovery Scale-Revised (CRS-R). We identified different time points: injury onset (t<sub>0</sub>), first blood sampling at admission in Neurorehabilitation (t<sub>1</sub>), and second blood sampling at discharge (t<sub>2</sub>). Patients were split into improved (improved level of consciousness from t<sub>1</sub> to t<sub>2</sub>) and not-improved (unchanged or worsened level of consciousness from t<sub>1</sub> to t<sub>2</sub>). Results: All biomarker levels decreased over time, even though each biomarker reveals typical features. Serum GFAP showed a weak correlation between t<sub>1</sub> and t<sub>2</sub> (<i>p</i> = 0.001), while no correlation was observed for serum NF-L (<i>p</i> = 0.955), UCH-L1 (<i>p</i> = 0.693), and T-Tau (<i>p</i> = 0.535) between t<sub>1</sub> and t<sub>2</sub>. Improved patients showed a significant decrease in the level of NF-L (<i>p</i> = 0.0001), UCH-L1 (<i>p</i> = 0.001), and T-Tau (<i>p</i> = 0.002), but not for serum GFAP (<i>p</i> = 0.283). No significant statistical differences were observed in the not-improved group. Conclusions: A significant correlation was found between the level of consciousness improvement and decreased NF-L, UCH-L1, and T-Tau levels. Future studies on the association of serum biomarkers with neurophysiological and neuroimaging prognostic indicators are recommended. |
| first_indexed | 2024-04-24T18:29:15Z |
| format | Article |
| id | doaj.art-9d115c3d21dd41f490803e9b9eae63eb |
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| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-04-24T18:29:15Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-9d115c3d21dd41f490803e9b9eae63eb2024-03-27T13:28:44ZengMDPI AGBrain Sciences2076-34252024-02-0114323910.3390/brainsci14030239The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary StudyRita Formisano0Mariagrazia D’Ippolito1Marco Giustini2Sheila Catani3Stefania Mondello4Iliana Piccolino5Filomena Iannuzzi6Kevin K. Wang7Ronald L. Hayes8Neurorehabilitation 2, Post-Coma Unit, IRCCS Fondazione Santa Lucia, 00179 Rome, ItalyNeurorehabilitation 2, Post-Coma Unit, IRCCS Fondazione Santa Lucia, 00179 Rome, ItalyEnvironmental and Social Epidemiology Unit, National Institute of Health, 00161 Rome, ItalyMultiple Sclerosis Unit, IRCCS Fondazione Santa Lucia, 00179 Rome, ItalyDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 98122 Messina, ItalyExperimental Neuro-Psychobiology Laboratory, IRCCS Fondazione Santa Lucia, 00179 Rome, ItalyExperimental Neuro-Psychobiology Laboratory, IRCCS Fondazione Santa Lucia, 00179 Rome, ItalyDepartment of Neurobiology, Center for Neurotrauma, Multiomics & Biomarkers (CNMB), Neuroscience Institute, Morehouse School of Medicine, Atlanta, GE 30310, USABanyan Biomarkers Inc., Alachua, FL 32615, USAIntroduction: Serum biomarkers, such as Neurofilament Light (NF-L), Glial Fibrillary Acidic Protein (GFAP), Ubiquitin C-terminal Hydrolase (UCH-L1), and Total-tau (T-Tau) have been proposed for outcome prediction in the acute phase of severe traumatic brain injury, but they have been less investigated in patients with prolonged DoC (p-DoC). Methods: We enrolled 25 p-DoC patients according to the Coma Recovery Scale-Revised (CRS-R). We identified different time points: injury onset (t<sub>0</sub>), first blood sampling at admission in Neurorehabilitation (t<sub>1</sub>), and second blood sampling at discharge (t<sub>2</sub>). Patients were split into improved (improved level of consciousness from t<sub>1</sub> to t<sub>2</sub>) and not-improved (unchanged or worsened level of consciousness from t<sub>1</sub> to t<sub>2</sub>). Results: All biomarker levels decreased over time, even though each biomarker reveals typical features. Serum GFAP showed a weak correlation between t<sub>1</sub> and t<sub>2</sub> (<i>p</i> = 0.001), while no correlation was observed for serum NF-L (<i>p</i> = 0.955), UCH-L1 (<i>p</i> = 0.693), and T-Tau (<i>p</i> = 0.535) between t<sub>1</sub> and t<sub>2</sub>. Improved patients showed a significant decrease in the level of NF-L (<i>p</i> = 0.0001), UCH-L1 (<i>p</i> = 0.001), and T-Tau (<i>p</i> = 0.002), but not for serum GFAP (<i>p</i> = 0.283). No significant statistical differences were observed in the not-improved group. Conclusions: A significant correlation was found between the level of consciousness improvement and decreased NF-L, UCH-L1, and T-Tau levels. Future studies on the association of serum biomarkers with neurophysiological and neuroimaging prognostic indicators are recommended.https://www.mdpi.com/2076-3425/14/3/239acquired brain injuryprolonged disorders of consciousnessserum biomarkers |
| spellingShingle | Rita Formisano Mariagrazia D’Ippolito Marco Giustini Sheila Catani Stefania Mondello Iliana Piccolino Filomena Iannuzzi Kevin K. Wang Ronald L. Hayes The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study Brain Sciences acquired brain injury prolonged disorders of consciousness serum biomarkers |
| title | The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study |
| title_full | The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study |
| title_fullStr | The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study |
| title_full_unstemmed | The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study |
| title_short | The Prognostic Role of Candidate Serum Biomarkers in the Post-Acute and Chronic Phases of Disorder of Consciousness: A Preliminary Study |
| title_sort | prognostic role of candidate serum biomarkers in the post acute and chronic phases of disorder of consciousness a preliminary study |
| topic | acquired brain injury prolonged disorders of consciousness serum biomarkers |
| url | https://www.mdpi.com/2076-3425/14/3/239 |
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