Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation
The use of perfluorane for the cytoprotection of lung structures in gram-negative lung infections has been inadequately studied. Objective: to experimentally reveal the pattern of morphological changes in the lung during combined inhalation of lipopolysaccharide (LPS) and perfluorane that has cytopr...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2015-02-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1435 |
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| author | A. M. Golubev A. N. Kuzovlev D. V. Sundukov M. A. Golubev |
| author_facet | A. M. Golubev A. N. Kuzovlev D. V. Sundukov M. A. Golubev |
| author_sort | A. M. Golubev |
| collection | DOAJ |
| description | The use of perfluorane for the cytoprotection of lung structures in gram-negative lung infections has been inadequately studied. Objective: to experimentally reveal the pattern of morphological changes in the lung during combined inhalation of lipopolysaccharide (LPS) and perfluorane that has cytoprotective properties. Materials and methods. The experiment was carried out on 35 outbred male albino rats weighing 320—350 g. The experimental animals were allocated to groups: 1) one-hour ventilation (controls) (n=5); 2) inhaled LPS 1.0 mg (n=10); 3) inhaled perfluorane 1.0 ml (n=10); 4) inhaled LPS 1.0 mg and then inhaled perfluorane 1.0 ml (n=10). Results. According to morphological findings, Group 1 (controls) was found to have no signs of lung structural changes. Group 2 (inhaled LPS) was noted to have signs of characteristic LPS-induced lung injury. Group 3 (inhaled perfluorane) showed complexes of macrophages with vacuolated cytoplasm (perfluorophages) in the bronchial lumen 3 hours after inhaled perfluorane administration. In Group 4, inhaled perfluorane exerted a cytoprotective effect: the degree of LPS- induced morphological changes in the lung was much lower than that in Group 2. Conclusion. The experimental model of LPS-induced lung injury indicated that LPS inhalation led to alveolar and bronchial epithelial damage, interstitial and alveolar edema, and obvious cell infiltration at the expense of lymphocytes, segmented leukocytes, and macrophages. This model showed that inhaled perfluorane reduced the signs of alveolar and bronchial epithelial damage and the degree of interstitial and alveolar edema. This supports clinical findings and offers possibilities of using inhaled perfluorane in resuscitation. |
| first_indexed | 2024-04-10T01:28:31Z |
| format | Article |
| id | doaj.art-9d117056e3dc4814816d133905b1154e |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2025-03-14T09:45:06Z |
| publishDate | 2015-02-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-9d117056e3dc4814816d133905b1154e2025-03-02T11:29:34ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102015-02-0111161310.15360/1813-9779-2015-1-6-131432Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane InhalationA. M. Golubev0A. N. Kuzovlev1D. V. Sundukov2M. A. Golubev3V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia 25, Petrovka, Build. 2, Moscow 107031V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia 25, Petrovka, Build. 2, Moscow 107031Peoples' Friendship University of Russia, Moscow, Russia 6, MiklukhoPeoples' Friendship University of Russia, Moscow, Russia 6, MiklukhoThe use of perfluorane for the cytoprotection of lung structures in gram-negative lung infections has been inadequately studied. Objective: to experimentally reveal the pattern of morphological changes in the lung during combined inhalation of lipopolysaccharide (LPS) and perfluorane that has cytoprotective properties. Materials and methods. The experiment was carried out on 35 outbred male albino rats weighing 320—350 g. The experimental animals were allocated to groups: 1) one-hour ventilation (controls) (n=5); 2) inhaled LPS 1.0 mg (n=10); 3) inhaled perfluorane 1.0 ml (n=10); 4) inhaled LPS 1.0 mg and then inhaled perfluorane 1.0 ml (n=10). Results. According to morphological findings, Group 1 (controls) was found to have no signs of lung structural changes. Group 2 (inhaled LPS) was noted to have signs of characteristic LPS-induced lung injury. Group 3 (inhaled perfluorane) showed complexes of macrophages with vacuolated cytoplasm (perfluorophages) in the bronchial lumen 3 hours after inhaled perfluorane administration. In Group 4, inhaled perfluorane exerted a cytoprotective effect: the degree of LPS- induced morphological changes in the lung was much lower than that in Group 2. Conclusion. The experimental model of LPS-induced lung injury indicated that LPS inhalation led to alveolar and bronchial epithelial damage, interstitial and alveolar edema, and obvious cell infiltration at the expense of lymphocytes, segmented leukocytes, and macrophages. This model showed that inhaled perfluorane reduced the signs of alveolar and bronchial epithelial damage and the degree of interstitial and alveolar edema. This supports clinical findings and offers possibilities of using inhaled perfluorane in resuscitation.https://www.reanimatology.com/rmt/article/view/1435critical conditionslipopolysaccharide-induced lung injuriesperfluoranecytoprotection |
| spellingShingle | A. M. Golubev A. N. Kuzovlev D. V. Sundukov M. A. Golubev Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation Общая реаниматология critical conditions lipopolysaccharide-induced lung injuries perfluorane cytoprotection |
| title | Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation |
| title_full | Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation |
| title_fullStr | Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation |
| title_full_unstemmed | Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation |
| title_short | Morphological Characteristics of the Lung during Lipopolysaccharide and Perfluorane Inhalation |
| title_sort | morphological characteristics of the lung during lipopolysaccharide and perfluorane inhalation |
| topic | critical conditions lipopolysaccharide-induced lung injuries perfluorane cytoprotection |
| url | https://www.reanimatology.com/rmt/article/view/1435 |
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