Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product
Epstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV <i...
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MDPI AG
2019-03-01
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| Online Access: | https://www.mdpi.com/1999-4915/11/3/285 |
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| author | Yusuke Yanagi H. M. Abdullah Al Masud Takahiro Watanabe Yoshitaka Sato Fumi Goshima Hiroshi Kimura Takayuki Murata |
| author_facet | Yusuke Yanagi H. M. Abdullah Al Masud Takahiro Watanabe Yoshitaka Sato Fumi Goshima Hiroshi Kimura Takayuki Murata |
| author_sort | Yusuke Yanagi |
| collection | DOAJ |
| description | Epstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV <i>Bam</i>HI S rightward reading frame 1 (BSRF1) encodes a tegument protein that, unlike its homologs herpes simplex virus unique long 51 (UL51) and human cytomegalovirus UL71, has not been extensively investigated. To examine the role of BSRF1, we prepared knockout and revertant strains using the bacterial artificial chromosome system. Unexpectedly, the disruption of the gene had little or no effect on EBV lytic replication and the transformation of primary B cells. However, the knockdown of BSRF1 in B95-8 cells decreased progeny production. An immunofluorescence assay revealed that BSRF1 localized to the Golgi apparatus in the cytoplasm, as did its homologs. BSRF1 also associated with <i>Bam</i>HI G leftward reading frame 3.5 (BGLF3.5), <i>Bam</i>HI B rightward reading frame 2 (BBRF2), and <i>Bam</i>HI A leftward reading frame 1 (BALF1), and BALF1 was incorporated into the tegument fraction with BSRF1. Taken together, our results indicate that BSRF1 plays a role in secondary envelopment or virion egress in the cytoplasm, as do its homolog genes. |
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| format | Article |
| id | doaj.art-9d135c7cfc1e43fd98470206c0d18867 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-12T12:07:27Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9d135c7cfc1e43fd98470206c0d188672022-12-22T00:24:58ZengMDPI AGViruses1999-49152019-03-0111328510.3390/v11030285v11030285Initial Characterization of the Epstein–Barr Virus BSRF1 Gene ProductYusuke Yanagi0H. M. Abdullah Al Masud1Takahiro Watanabe2Yoshitaka Sato3Fumi Goshima4Hiroshi Kimura5Takayuki Murata6Department of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanDepartment of Virology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, JapanEpstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV <i>Bam</i>HI S rightward reading frame 1 (BSRF1) encodes a tegument protein that, unlike its homologs herpes simplex virus unique long 51 (UL51) and human cytomegalovirus UL71, has not been extensively investigated. To examine the role of BSRF1, we prepared knockout and revertant strains using the bacterial artificial chromosome system. Unexpectedly, the disruption of the gene had little or no effect on EBV lytic replication and the transformation of primary B cells. However, the knockdown of BSRF1 in B95-8 cells decreased progeny production. An immunofluorescence assay revealed that BSRF1 localized to the Golgi apparatus in the cytoplasm, as did its homologs. BSRF1 also associated with <i>Bam</i>HI G leftward reading frame 3.5 (BGLF3.5), <i>Bam</i>HI B rightward reading frame 2 (BBRF2), and <i>Bam</i>HI A leftward reading frame 1 (BALF1), and BALF1 was incorporated into the tegument fraction with BSRF1. Taken together, our results indicate that BSRF1 plays a role in secondary envelopment or virion egress in the cytoplasm, as do its homolog genes.https://www.mdpi.com/1999-4915/11/3/285EBVBSRF1Golgi apparatussecondary envelopment |
| spellingShingle | Yusuke Yanagi H. M. Abdullah Al Masud Takahiro Watanabe Yoshitaka Sato Fumi Goshima Hiroshi Kimura Takayuki Murata Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product Viruses EBV BSRF1 Golgi apparatus secondary envelopment |
| title | Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product |
| title_full | Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product |
| title_fullStr | Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product |
| title_full_unstemmed | Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product |
| title_short | Initial Characterization of the Epstein–Barr Virus BSRF1 Gene Product |
| title_sort | initial characterization of the epstein barr virus bsrf1 gene product |
| topic | EBV BSRF1 Golgi apparatus secondary envelopment |
| url | https://www.mdpi.com/1999-4915/11/3/285 |
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