Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases
Dextransucrases are glucansucrases with broad applications in the food, cosmetics and pharmaceutical industries. Using sucrose as the glucosyl donor, they synthesize both high molecular weight (HMW) dextrans and potential prebiotic oligosaccharides. The process selectivity can be modulated by varyin...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2004-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/163422 |
| _version_ | 1827610273734721536 |
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| author | Arantza Gómez de Segura Miguel Alcalde Nieves López-Cortés Francisco J. Plou Antonio Ballesteros |
| author_facet | Arantza Gómez de Segura Miguel Alcalde Nieves López-Cortés Francisco J. Plou Antonio Ballesteros |
| author_sort | Arantza Gómez de Segura |
| collection | DOAJ |
| description | Dextransucrases are glucansucrases with broad applications in the food, cosmetics and pharmaceutical industries. Using sucrose as the glucosyl donor, they synthesize both high molecular weight (HMW) dextrans and potential prebiotic oligosaccharides. The process selectivity can be modulated by varying the reaction conditions. When no other molecule is present in the reaction, only dextrans are synthesized. In the presence of methyl α-D-glucopyranoside, the synthesis of methyl polyglucosides takes place, diminishing the transfer of glucose molecules to form dextran. In this work, the formation of HMW soluble dextran and methyl polyglucosides was studied with dextransucrases from Leuconostoc mesenteroides, strains B-512F and B-1299. The amount of dextran formed with dextransucrase B-512F was reduced up to 4 % with respect to the control in absence of acceptor, using a mass ratio of sucrose:methyl α-D-glucopyranoside of 1:4. The encapsulation in alginate retains the dextran inside the beads, causing a distortion of the biocatalyst and finally releasing the polysaccharides into the reaction medium. |
| first_indexed | 2024-03-09T07:46:56Z |
| format | Article |
| id | doaj.art-9d140d3227e44a41a21c719e30afd886 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:46:56Z |
| publishDate | 2004-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-9d140d3227e44a41a21c719e30afd8862023-12-03T02:57:39ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062004-01-01424337342Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated DextransucrasesArantza Gómez de Segura0Miguel Alcalde1Nieves López-Cortés2Francisco J. Plou3Antonio Ballesteros4Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Marie Curie 2, E-28049 Madrid, SpainDepartamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Marie Curie 2, E-28049 Madrid, SpainDepartamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Marie Curie 2, E-28049 Madrid, SpainDepartamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Marie Curie 2, E-28049 Madrid, SpainDepartamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Marie Curie 2, E-28049 Madrid, SpainDextransucrases are glucansucrases with broad applications in the food, cosmetics and pharmaceutical industries. Using sucrose as the glucosyl donor, they synthesize both high molecular weight (HMW) dextrans and potential prebiotic oligosaccharides. The process selectivity can be modulated by varying the reaction conditions. When no other molecule is present in the reaction, only dextrans are synthesized. In the presence of methyl α-D-glucopyranoside, the synthesis of methyl polyglucosides takes place, diminishing the transfer of glucose molecules to form dextran. In this work, the formation of HMW soluble dextran and methyl polyglucosides was studied with dextransucrases from Leuconostoc mesenteroides, strains B-512F and B-1299. The amount of dextran formed with dextransucrase B-512F was reduced up to 4 % with respect to the control in absence of acceptor, using a mass ratio of sucrose:methyl α-D-glucopyranoside of 1:4. The encapsulation in alginate retains the dextran inside the beads, causing a distortion of the biocatalyst and finally releasing the polysaccharides into the reaction medium.http://hrcak.srce.hr/file/163422glucosyltransferasesimmobilizationdextranacceptorprebiotics |
| spellingShingle | Arantza Gómez de Segura Miguel Alcalde Nieves López-Cortés Francisco J. Plou Antonio Ballesteros Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases Food Technology and Biotechnology glucosyltransferases immobilization dextran acceptor prebiotics |
| title | Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases |
| title_full | Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases |
| title_fullStr | Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases |
| title_full_unstemmed | Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases |
| title_short | Modulating the Synthesis of Dextran with the Acceptor Reaction Using Native and Encapsulated Dextransucrases |
| title_sort | modulating the synthesis of dextran with the acceptor reaction using native and encapsulated dextransucrases |
| topic | glucosyltransferases immobilization dextran acceptor prebiotics |
| url | http://hrcak.srce.hr/file/163422 |
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