Viability study of a multiplex diagnostic platform for Chagas disease
A new multiplex assay platform was evaluated to detect Trypanosoma cruzi infection using the recombinant antigens CRA, FRA, CRAFRA fusion and parasite lysate. The antigens presented different sensitivity and specificity in a singleplex test when compared to a serial dilution of two pools comprising...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-07-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000900019 |
| _version_ | 1797757707734745088 |
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| author | Leonardo Foti Bruna de Paula Fonseca e Fonseca Lilian Dias Nascimento Christiane de Fatima Silva Marques Edmilson Domingos da Silva Cesar Augusto Barros Duarte Christian M Probst Samuel Goldenberg Antônio Gomes Pinto Marco Aurélio Krieger |
| author_facet | Leonardo Foti Bruna de Paula Fonseca e Fonseca Lilian Dias Nascimento Christiane de Fatima Silva Marques Edmilson Domingos da Silva Cesar Augusto Barros Duarte Christian M Probst Samuel Goldenberg Antônio Gomes Pinto Marco Aurélio Krieger |
| author_sort | Leonardo Foti |
| collection | DOAJ |
| description | A new multiplex assay platform was evaluated to detect Trypanosoma cruzi infection using the recombinant antigens CRA, FRA, CRAFRA fusion and parasite lysate. The antigens presented different sensitivity and specificity in a singleplex test when compared to a serial dilution of two pools comprising 10 positive serum samples and one pool of 10 negative samples. The recombinant protein CRA presented lower sensitivity (55%) in contrast to the 100% specificity and sensitivity of FRA, CRAFRA and T. cruzi lysate. These antigens also showed good results in a duplex test and the duplex test with CRAFRA/T. cruzi lysate showed better performance with 100% specificity and sensitivity, as well as a lower cut-off value in comparison to the other duplex test, FRA/T. cruzi lysate. Hence, when the antigens were used in duplex format, both tests showed decreased cut-off values and no interference between different bead sets, resulting in increasing sensitivity and specificity. The results of these multiplex tests show that they could be an alternative to singleplex detection for Chagas disease, and also indicate the necessity of using multiplex diagnostic tools to increase the sensitivity and specificity for diagnostic tests. Emerging data from the T. cruzi genome and from its ORFeome project will also allow the identification of new antigens for this disease detection application. |
| first_indexed | 2024-03-12T18:19:25Z |
| format | Article |
| id | doaj.art-9d16277f07e14299846ab01be3a2d566 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:19:25Z |
| publishDate | 2009-07-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-9d16277f07e14299846ab01be3a2d5662023-08-02T09:00:00ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-07-0110413614110.1590/S0074-02762009000900019Viability study of a multiplex diagnostic platform for Chagas diseaseLeonardo FotiBruna de Paula Fonseca e FonsecaLilian Dias NascimentoChristiane de Fatima Silva MarquesEdmilson Domingos da SilvaCesar Augusto Barros DuarteChristian M ProbstSamuel GoldenbergAntônio Gomes PintoMarco Aurélio KriegerA new multiplex assay platform was evaluated to detect Trypanosoma cruzi infection using the recombinant antigens CRA, FRA, CRAFRA fusion and parasite lysate. The antigens presented different sensitivity and specificity in a singleplex test when compared to a serial dilution of two pools comprising 10 positive serum samples and one pool of 10 negative samples. The recombinant protein CRA presented lower sensitivity (55%) in contrast to the 100% specificity and sensitivity of FRA, CRAFRA and T. cruzi lysate. These antigens also showed good results in a duplex test and the duplex test with CRAFRA/T. cruzi lysate showed better performance with 100% specificity and sensitivity, as well as a lower cut-off value in comparison to the other duplex test, FRA/T. cruzi lysate. Hence, when the antigens were used in duplex format, both tests showed decreased cut-off values and no interference between different bead sets, resulting in increasing sensitivity and specificity. The results of these multiplex tests show that they could be an alternative to singleplex detection for Chagas disease, and also indicate the necessity of using multiplex diagnostic tools to increase the sensitivity and specificity for diagnostic tests. Emerging data from the T. cruzi genome and from its ORFeome project will also allow the identification of new antigens for this disease detection application.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000900019Chagas diseasediagnosticmultiplexbead-based assayTrypanosoma cruzi |
| spellingShingle | Leonardo Foti Bruna de Paula Fonseca e Fonseca Lilian Dias Nascimento Christiane de Fatima Silva Marques Edmilson Domingos da Silva Cesar Augusto Barros Duarte Christian M Probst Samuel Goldenberg Antônio Gomes Pinto Marco Aurélio Krieger Viability study of a multiplex diagnostic platform for Chagas disease Memorias do Instituto Oswaldo Cruz Chagas disease diagnostic multiplex bead-based assay Trypanosoma cruzi |
| title | Viability study of a multiplex diagnostic platform for Chagas disease |
| title_full | Viability study of a multiplex diagnostic platform for Chagas disease |
| title_fullStr | Viability study of a multiplex diagnostic platform for Chagas disease |
| title_full_unstemmed | Viability study of a multiplex diagnostic platform for Chagas disease |
| title_short | Viability study of a multiplex diagnostic platform for Chagas disease |
| title_sort | viability study of a multiplex diagnostic platform for chagas disease |
| topic | Chagas disease diagnostic multiplex bead-based assay Trypanosoma cruzi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000900019 |
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